GB/T 31520-2015 English PDFUS$169.00 · In stock
Delivery: <= 3 days. True-PDF full-copy in English will be manually translated and delivered via email. GB/T 31520-2015: Determination of astaxanthin in Haematococcus -- High performance liquid chromatography method Status: Valid
Basic dataStandard ID: GB/T 31520-2015 (GB/T31520-2015)Description (Translated English): Determination of astaxanthin in Haematococcus -- High performance liquid chromatography method Sector / Industry: National Standard (Recommended) Classification of Chinese Standard: X20 Classification of International Standard: 67.120.30 Word Count Estimation: 8,863 Date of Issue: 2015-05-15 Date of Implementation: 2015-05-15 Quoted Standard: GB/T 6682 Regulation (derived from): National Standard Announcement 2015 No. 15 Issuing agency(ies): General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China, Standardization Administration of the People's Republic of China Summary: This Standard specifies the method of liquid chromatography in pluvialis astaxanthin. This Standard applies to the determination of astaxanthin content of algae powder algae pluvialis. GB/T 31520-2015: Determination of astaxanthin in Haematococcus -- High performance liquid chromatography method---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order. Determination of astaxanthin in Haematococcus. High performance liquid chromatography method ICS 67.120.30 X20 National Standards of People's Republic of China Determination of Haematococcus astaxanthin Liquid chromatography Issued on. 2015-05-15 2015-05-15 implementation Administration of Quality Supervision, Inspection and Quarantine of People's Republic of China Standardization Administration of China released ForewordThis standard was drafted in accordance with GB/T 1.1-2009 given rules. The standard proposed by the People's Republic of China Ministry of Agriculture. This standard by the National Standardization Technical Committee of Fisheries Aquatic Products Processing Technical Committee (SAC/TC156/SC3) centralized. This standard was drafted. Chinese Academy of Fishery Sciences Yellow Sea Fisheries Research Institute, Yunnan Ireland and Biotechnology Limited. The main drafters of this standard. Red Sun Wei, Zhang Yong, Liang Kai cold, Chun Mei, Liu Jianguo, Wu Qiujin, Xing Lihong, Quebec seedlings are, Xiaorong Hui, Sun tai, Zhaiyu Xiu, Xu Yang. Determination of Haematococcus astaxanthin Liquid chromatography1 ScopeThis standard specifies the liquid chromatographic method Haematococcus (Haematococcus) in astaxanthin. This standard applies to the determination of algae powder astaxanthin content pluvialis algae (Haematococcuspluvialis).2 Normative referencesThe following documents for the application of this document is essential. For dated references, only the dated version suitable for use herein Member. For undated references, the latest edition (including any amendments) applies to this document. Laboratory use specifications and test methods GB/T 6682 Analysis Principle 3 The sample was extracted in methylene chloride and methanol mixed solution, methanol solution of sodium hydroxide saponified, so that one of astaxanthin ester was converted into a free form Liquid chromatograph astaxanthin, after C30 reverse phase HPLC column separated, with a UV detector and external standard.4 ReagentsThe reagents used Unless otherwise specified, are HPLC grade. 4.1 test water should be consistent with GB/T 6682 requested a water. 4.2 acetone. 4.3 phosphoric acid. pure class distinctions. 4.4 Sodium hydroxide. pure class distinctions. 4.5 methanol. 4.6 dichloromethane. 4.7 tert-butyl methyl ether. 4.8 2,6 BHT. chemically pure. 4.9 1% phosphoric acid solution (volume fraction). volume of 10mL phosphoric acid (4.3) and 990mL of water, mix and reserve. 4.10 methylene chloride - methanol solution. Measure 250mL dichloromethane and 750mL of methanol was added 0.5g2,6- BHT (4.8), mix and reserve. 4.11 0.1mol/L sodium hydroxide - methanol solution. Weigh 0.4g of sodium hydroxide (4.4), dissolved in methanol and diluted to 100mL, after mixing spare. 4.12 2% phosphoric acid - methanol solution (volume fraction). volume of 2mL phosphoric acid (4.3) and 98mL of methanol, mix and reserve. 4.13 all-trans-astaxanthin, 13-cis-astaxanthin, 9-cis-astaxanthin standard. Purity ≥95%. 4.14 all-trans astaxanthin standard stock solution. Weigh accurately all-trans astaxanthin standard about 10mg, dissolved in acetone and set the volume to 500mL flask, and this solution at a concentration of 20μg/mL, nitrogen sealed, -18 ℃ refrigerator in the dark, valid for one month. ......Tips & Frequently Asked Questions:Question 1: How long will the true-PDF of GB/T 31520-2015_English be delivered?Answer: Upon your order, we will start to translate GB/T 31520-2015_English as soon as possible, and keep you informed of the progress. The lead time is typically 1 ~ 3 working days. The lengthier the document the longer the lead time.Question 2: Can I share the purchased PDF of GB/T 31520-2015_English with my colleagues?Answer: Yes. The purchased PDF of GB/T 31520-2015_English will be deemed to be sold to your employer/organization who actually pays for it, including your colleagues and your employer's intranet.Question 3: Does the price include tax/VAT?Answer: Yes. 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