GB/T 30987-2020 English PDF

US$409.00 · In stock
Delivery: <= 4 days. True-PDF full-copy in English will be manually translated and delivered via email.
GB/T 30987-2020: Determination of free amino acids in plant
Status: Valid

GB/T 30987: Historical versions

Standard ID	USD	BUY PDF	Lead-Days	Standard Title (Description)	Status
GB/T 30987-2020	409	Add to Cart	4 days	Determination of free amino acids in plant	Valid
GB/T 30987-2014	519	Add to Cart	3 days	Determination of free amino acids in plants	Obsolete

Similar standards

GB/T 38505   GB/T 38132   GB/T 36081   GB/T 33411   GB/T 43708   

Basic data

Standard ID: GB/T 30987-2020 (GB/T30987-2020)
Description (Translated English): Determination of free amino acids in plant
Sector / Industry: National Standard (Recommended)
Classification of Chinese Standard: A40
Classification of International Standard: 07.080
Word Count Estimation: 22,296
Date of Issue: 2020-03-31
Date of Implementation: 2020-03-31
Issuing agency(ies): State Administration for Market Regulation, China National Standardization Administration

GB/T 30987-2020: Determination of free amino acids in plant

---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Determination of free amino acids in plant ICS 07.080 A40 National Standards of People's Republic of China Replace GB/T 30987-2014 Determination of free amino acids in plants 2020-03-31 release 2020-03-31 Implementation State Administration of Market Supervision and Administration Issued by the National Standardization Management Committee

Contents

Foreword I 1 Scope 1 2 Normative references 1 3 Terms and definitions 1 4 Fully automatic amino acid analyzer method (first method) 1 5 High performance liquid chromatography (second method) 6 6 Liquid chromatography-tandem mass spectrometry (third method) 9 Appendix A (Informative Appendix) Amino Acid Compound Information and Quantitation Limit 13 Appendix B (informative appendix) Chromatogram of 21 kinds of amino acid mixed standard working solution 14 Appendix C (informative appendix) Liquid chromatography-tandem mass spectrometer parameter reference conditions 18

Foreword

This standard was drafted in accordance with the rules given in GB/T 1.1-2009. This standard replaces GB/T 30987-2014 "Determination of Free Amino Acids in Plants". Compared with GB/T 30987-2014, the main technical changes are as follows. --- Added liquid chromatography-tandem mass spectrometry (see Chapter 6); --- Modified the method of sensitivity expression (see 4.5.5.6, 5.5.6.4,.2014 edition 4.10, 5.10). This standard is proposed and managed by the National Biochemical Testing Standardization Technical Committee (SAC/TC387). This standard was drafted by. Institute of Biology, China Institute of Test Technology, China Institute of Test Technology, Hebei Food Inspection Institute. The main drafters of this standard. Li Huaiping, Tang Xiangkai, Yang Jiebin, Zou Yan, Zhao Aiping, Wu Wei, Zhang Yan. The previous versions of the standard replaced by this standard are as follows. --- GB/T 30987-2014. Determination of free amino acids in plants

1 Scope

This standard specifies the method for determining the content of free amino acids in plants, including the automatic amino acid analyzer method (first method), high-performance liquid phase Chromatograph method (second method) and liquid chromatography-tandem mass spectrometry (third method) three methods. This standard applies to 21 free amino acids (alanine, arginine, asparagine, asparagus) in tea, Chinese herbal medicines, tobacco leaves and other plant samples Leucine, cystine, γ-aminobutyric acid, glutamine, glutamic acid, glycine, histidine, isoleucine, leucine, lysine, methionine, phenylpropyl Determination of amino acid, proline, serine, theanine, threonine, tyrosine, valine).

2 Normative references

The following documents are essential for the application of this document. For dated references, only the dated version applies to this article Pieces. For the cited documents without date, the latest version (including all amendments) applies to this document. GB/T 6682 Analysis laboratory specifications and test methods GB/T 8303-2013 Preparation of tea ground samples and determination of dry matter content

3 Terms and definitions

The following terms and definitions apply to this document. 3.1 Free aminoacids The plant is not combined with other chemical substances, exists in the form of a single molecule or ion, and can be directly leached out of an aqueous solution or other solvent Of amino acids. 4 Fully automatic amino acid analyzer method (first method) 4.1 Principle After the free amino acid in the sample is extracted by boiling water, it is separated by the sulfonic acid cation exchange column of the amino acid analyzer, and then added at 135 ℃ Heat, mixed reaction of amino acids and ninhydrin, primary amine and ninhydrin produce blue-purple compounds, secondary amine and ninhydrin produce yellow compounds, respectively in The two derivative products are detected by visible light spectrophotometer at 570nm and 440nm wavelengths, the retention time is qualitative, and the external standard working curve Method quantitative. 4.2 Reagents or materials Unless otherwise specified, the reagents used in this method are of superior grade. Water is the first-grade water in accordance with GB/T 6682. 4.2.1 Lithium citrate (tetrahydrate). 4.2.2 Lithium chloride. 4.2.3 Citric acid. 4.2.4 Lithium hydroxide. 4.2.5 Sodium borohydride. ......

Image     

Tips & Frequently Asked Questions:

Question 1: How long will the true-PDF of GB/T 30987-2020_English be delivered?

Answer: Upon your order, we will start to translate GB/T 30987-2020_English as soon as possible, and keep you informed of the progress. The lead time is typically 2 ~ 4 working days. The lengthier the document the longer the lead time.

Question 2: Can I share the purchased PDF of GB/T 30987-2020_English with my colleagues?

Answer: Yes. The purchased PDF of GB/T 30987-2020_English will be deemed to be sold to your employer/organization who actually pays for it, including your colleagues and your employer's intranet.

Question 3: Does the price include tax/VAT?

Answer: Yes. Our tax invoice, downloaded/delivered in 9 seconds, includes all tax/VAT and complies with 100+ countries' tax regulations (tax exempted in 100+ countries) -- See Avoidance of Double Taxation Agreements (DTAs): List of DTAs signed between Singapore and 100+ countries

Question 4: Do you accept my currency other than USD?

Answer: Yes. If you need your currency to be printed on the invoice, please write an email to Sales@ChineseStandard.net. In 2 working-hours, we will create a special link for you to pay in any currencies. Otherwise, follow the normal steps: Add to Cart -- Checkout -- Select your currency to pay.

Question 5: Should I purchase the latest version GB/T 30987-2020?

Answer: Yes. Unless special scenarios such as technical constraints or academic study, you should always prioritize to purchase the latest version GB/T 30987-2020 even if the enforcement date is in future. Complying with the latest version means that, by default, it also complies with all the earlier versions, technically.