GB/T 30744-2014 English PDF

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GB/T 30744-2014: The technology specification for the pre-treatment of deep-sea microorganism samples
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Basic data

Standard ID: GB/T 30744-2014 (GB/T30744-2014)
Description (Translated English): The technology specification for the pre-treatment of deep-sea microorganism samples
Sector / Industry: National Standard (Recommended)
Classification of Chinese Standard: A45
Classification of International Standard: 07.060
Word Count Estimation: 39,339
Date of Issue: 6/9/2014
Date of Implementation: 10/1/2014
Quoted Standard: GB/T 12763.4; GB/T 12763.6; HY/T 058
Regulation (derived from): National Standards Bulletin No. 11 of 2014
Issuing agency(ies): General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China, Standardization Administration of the People's Republic of China
Summary: This Standard specifies the deep-sea microbial samples before processing the technical requirements, working conditions, on-site treatment of deep-sea samples, microbiological and genetic material extraction, storage and data processing. This Standard app

GB/T 30744-2014: The technology specification for the pre-treatment of deep-sea microorganism samples

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The technology specification for the pre-treatment of deep-sea microorganism samples ICS 07.060 A45 National Standards of People's Republic of China Deep-sea microbial samples before processing technology specification samples Issued on. 2014-06-09 2014-10-01 implementation Administration of Quality Supervision, Inspection and Quarantine of People's Republic of China Standardization Administration of China released

Table of Contents

Preface Ⅰ 1 Scope 1 2 Normative references 1 3 Terms, Definitions and Abbreviations 1 4 Reagents and materials 2 Instruments and equipment 5 5 6 GENERAL PROVISIONS 6 7 Sample scene 6 8 deep-sea microbial strains isolated 8 9 deep-sea microbial genome DNA extraction 10 10 deep-sea microbial metagenomic DNA extracted 12 11 deep-sea biological sample preservation and microbial resources 13 Appendix A (informative) before deep-sea microbial sample record sheet format 22 Annex B (informative) pressure isolates 28 Annex C (informative) Molecular identification of microbial strains 29 Annex D (informative) metagenomic Cosmid library construction 32 Table A.1 sample deep-sea sampling record sheet 22 Isolation and identification of deep-sea microbes Table A.2 Table 22 records Table A.3 deep-sea microbial genome DNA extraction record Table 23 Table A.4 deep sea samples metagenomic DNA extracted record Table 23 Table A.5 deep sea samples deposited record Table 24 Table A.6 deep-sea bacteria deposited record Table 24 Table A.7 deep sea actinomycetes deposited record Table 25 Table A.8 deep sea fungus deposited record Table 26 Table A.9 deep sea samples metagenomic DNA library records Table 27 Scope Table C.1 16SrRNA gene universal primers 29

Foreword

This standard was drafted in accordance with GB/T 1.1-2009 given rules. This standard was proposed by the State Oceanic Administration. This standard by the National Standardization Technical Commission for Oceanography (SAC/TC283) centralized. This standard was drafted. Third Institute of Oceanography, State Oceanic Administration. The main drafters of this standard. Zeng Yun Ying OCEANOGRAPHY Ip Tak Chan, Chen Xinhua, Lin Rongcheng, Sunfeng Qin, Xu Li Mei, Bao Ying cover. Deep-sea microbial samples before processing technology specification

1 Scope

This standard specifies the deep-sea microbial samples pre-treatment of the technical requirements, working conditions, deep sea sample scene, microbiology and genetic Material extraction, storage and data processing. This standard applies to depths greater than or equal to the scene to deal with deep-sea microbial samples 1000m extracted and saved.

2 Normative references

The following documents for the application of this document is essential. For dated references, only the dated version suitable for use herein Member. For undated references, the latest edition (including any amendments) applies to this document. GB/T 12763.4 specification for oceanographic survey - Part 4. Chemical elements Marine Survey GB/T 12763.6 specification for oceanographic survey - Part 6. Marine Biological Survey HY/T 058 oceanographic survey observations to monitor the archives specification 3 Terms, definitions and abbreviations The following terms, definitions and abbreviations apply to this document. 3.1 Terms and Definitions 3.1.1 Deep Sea deepsea Greater than or equal to 1000m depth marine areas. 3.1.2 Microbial samples microorganismsamples We have trained or extraction and purification, has certain scientific significance, actual or potential economic value, can be directly applied to various scientific Various strains studied genetic material (including bacteria, fungi, actinomycetes), DNA/RNA and the like; and strains containing said genetic material and Deep-sea samples, including water samples, sediment samples, rock samples and the like. 3.1.3 Pre-treatment pre-treatment Various processes prior to engaging in deep-sea microbial research collected from a variety of deep-sea microbial samples (see 3.1.2) carried out, including Including. deep-sea biological sample scene, deep-sea microbial strains isolated, deep-sea microbial genome DNA extraction, deep-sea microbial metagenome DNA extraction, deep-sea biological samples and microbial resources preservation. 3.1.4 Low microbial cold-adaptedmicroorganism Good growth of microorganisms (including bacteria, fungi, actinomycetes) at a temperature below 37 ℃, the optimum growth temperature equal to or lower than 25 ℃. 3.1.5 Thermophile thermophilic microorganisms Good growth of microorganisms (including bacteria, fungi, actinomycetes) equal to or higher than 55 ℃ temperatures. ......

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