GB/T 30737-2014 English PDF

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GB/T 30737-2014: Determination of marine photosynthetic picoplankton -- Flow cytometry
Status: Valid

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Basic data

Standard ID: GB/T 30737-2014 (GB/T30737-2014)
Description (Translated English): Determination of marine photosynthetic picoplankton -- Flow cytometry
Sector / Industry: National Standard (Recommended)
Classification of Chinese Standard: A45
Classification of International Standard: 07.060
Word Count Estimation: 14,196
Date of Issue: 6/9/2014
Date of Implementation: 10/1/2014
Quoted Standard: GB/T 12763.6-2007
Regulation (derived from): National Standards Bulletin No. 11 of 2014
Issuing agency(ies): General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China, Standardization Administration of the People's Republic of China
Summary: This Standard specifies the marine photosynthetic plankton pico Central Prochlorococcus, Synechococcus and pico-cell numbers eukaryotic algae flow cytometry assay. This Standard applies to these three major groups of marine number of cells was measured by

GB/T 30737-2014: Determination of marine photosynthetic picoplankton -- Flow cytometry

---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Determination of marine photosynthetic picoplankton.Flow cytometry ICS 07.060 A45 National Standards of People's Republic of China Determination of photosynthetic plankton in the ocean pico Flow cytometry assay Flowcytometry Issued on. 2014-06-09 2014-10-01 implementation Administration of Quality Supervision, Inspection and Quarantine of People's Republic of China Standardization Administration of China released

Table of Contents

Preface Ⅰ 1 Scope 1 2 Normative references 1 3 Terms and definitions 4 principle of the method 2 The main equipment 2 5 6 Reagent 2 7 Sample collection and processing 3 8 measuring step 3 9 Analysis and Calculation 4 Appendix A (normative) Determination of the ocean pico plankton photosynthesis flow cytometry assay sampling and assay results are reported in Table 7 format Reference 10

Foreword

This standard was drafted in accordance with GB/T 1.1-2009 given rules. This standard was proposed by the State Oceanic Administration. This standard by the National Standardization Technical Commission for Oceanography (SAC/TC283) centralized. This standard was drafted. South China Sea Environmental Monitoring Center, State Oceanic Administration, Xiamen University, State Oceanic Administration, Second Institute of Oceanography, East China Normal University, South China Sea Institute of Oceanology. The main drafters of this standard. Zhu Yijia, Jiao Zhi, Huang Chuguang, Luoting Wei, Tang Hong, Yue Feng Feng, Qiu Dajun, Li Haitao, Yang Zhenxiong, forest side, HTC square, Xu Chau war, Syria Cai Wei, Qu read East. Determination of photosynthetic plankton in the ocean pico Flow cytometry assay

1 Scope

This standard specifies the ocean pico plankton photosynthesis Central Prochlorococcus, Synechococcus and eukaryotic algal cells slightly streaming fine amount Cellular assays. This standard applies to the three major groups of the number of cells was measured by flow cytometry ocean pico photosynthetic plankton.

2 Normative references

The following documents for the application of this document is essential. For dated references, only the dated version suitable for use herein Member. For undated references, the latest edition (including any amendments) applies to this document. GB/T 12763.6-2007 oceanographic survey - Part 6. Marine Biological Survey

3 Terms and Definitions

GB/T 12763.6-2007 and as defined in the following terms and definitions apply to this document. 3.1 Plankton plankton Lack of well-developed organ movement, no or only weak athletic ability, suspended in the water layer, often with the water moving creatures. NOTE. rewrite GB/T 12763.6-2007, definitions 3.9. 3.2 Picoplankton picoplankton Range of particle size 0.2μm ~ 2μm plankton. Note 1. picoplankton is not a strictly biological concept, but a concept of ecology. Note 2. rewrite GB/T 12763.6-2007, definitions 3.9. 3.3 Pico photosynthetic plankton photosyntheticpicoplankton Can photosynthetic picoplankton. NOTE. pico plankton photosynthesis and autotrophic and heterotrophic include two types of self-support type predominant. Autotrophic photosynthetic plankton by a slight The three main groups. the Prochlorococcus, Synechococcus and pico eukaryotic algae. Heterotrophic photosynthetic plankton slightly mainly Anoxygenic photosynthetic fine Bacteria (anoxygenicphototrophicbacteria, APB). 3.4 Prochlorococcus Prochlorococcusspp;. Pro. Blue bacteria (Cyanobacteria) in a genus of prokaryotes, the particle size is generally about 0.6μm, its main feature is the pigment divinyl Group chlorophyll (DiVinyl-Chlorophyl, DVChl). 3.5 Synechococcus Synechococcusspp;. Syn. A genus of cyanobacteria, the prokaryotes, the particle size is generally about 1μm, in addition to containing chlorophyll a (Chlorophyla), its main ......

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