GB/T 29888-2013 English PDFUS$169.00 ยท In stock
Delivery: <= 3 days. True-PDF full-copy in English will be manually translated and delivered via email. GB/T 29888-2013: General requirements of DNA array-based mycobacteria identification Status: Valid
Basic dataStandard ID: GB/T 29888-2013 (GB/T29888-2013)Description (Translated English): General requirements of DNA array-based mycobacteria identification Sector / Industry: National Standard (Recommended) Classification of Chinese Standard: C30 Classification of International Standard: 11.040.55 Word Count Estimation: 7,785 Quoted Standard: YY/T 1153; YY/T 1154 Regulation (derived from): National Standards Bulletin No. 22 of 2013 Issuing agency(ies): General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China, Standardization Administration of the People's Republic of China Summary: This standard specifies the basic requirements for the identification of Mycobacterium species microarray detection. This standard applies to species identification of pathogenic mycobacteria in clinical or clinical often isolated from the non-pathogenic GB/T 29888-2013: General requirements of DNA array-based mycobacteria identification---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.General requirements of DNA array-based mycobacteria identification ICS 11.040.55 C30 National Standards of People's Republic of China Mycobacterial species identification of gene microarray basic requirements Issued on. 2013-11-12 2014-04-11 implementation Administration of Quality Supervision, Inspection and Quarantine of People's Republic of China Standardization Administration of China released ForewordThis standard was drafted in accordance with GB/T 1.1-2009 given rules. The standard proposed by the Boao Biological Co., Ltd. and the China Disease Prevention and Control Center. This standard by the National Standardization Technical Committee biochip (SAC/TC421) centralized. This standard was drafted. Boao Biological Co., Ltd., China Center for Disease Control and Prevention. The main drafters of this standard. Guo, I wish to make incense, Zhao Yanlin, Liu Yingying, Wang Can. Mycobacterial species identification of gene microarray basic requirements1 ScopeThis standard specifies the basic requirements for identification of mycobacterial species microarray detection. This standard applies to species identification of pathogenic mycobacteria in clinical or clinical often isolated from non-pathogenic mycobacteria.2 Normative referencesThe following documents for the application of this document is essential. For dated references, only the dated version suitable for use herein Member. For undated references, the latest edition (including any amendments) applies to this document. YY/T 1153 in vitro diagnostic use DNA microarrays YY/T 1154 confocal laser scanner3 AbbreviationsThe following abbreviations apply to this document. DNA. deoxyribonucleic acid; PCR. polymerase chain reaction. Principle 4 Design of specific PCR amplification primers and species-specific oligonucleotide probes based on common clinical mycobacteria. In clinical samples Mycobacteria isolated DNA as a template, using PCR amplification techniques. Since the end of the primer with luminescent markers, and therefore During amplification the DNA molecule to be detected is amplified DNA fragment with luminescent markers. The luminescent markers with PCR amplification product with a probe on the chip under certain conditions, a hybridization reaction, based on the nucleotide complementary Pairing rules, matching sequence PCR products with a probe to form a stable secondary structures. Probe amplification product based on a combination of PCR Specific locations on the chip arrangement, we can infer the corresponding test bacteria-related information, identify the type of test bacteria.5 Materials and Reagents5.1 Apparatus and materials 5.1.1 microarray scanner. should comply with YY/T 1154 requirements and obtaining medical device registration certificate. 5.1.2 microarray hybridization cassette. 5.1.3 nucleic acid amplification. 5.1.4 microarray hybridization instrument (optional). 5.1.5 chip dry cleaning instrument (optional). 5.1.6 micro pipette and pipette tips. 5.1.7 constant temperature water bath. 5.1.8 microcentrifuge. ......Tips & Frequently Asked Questions:Question 1: How long will the true-PDF of GB/T 29888-2013_English be delivered?Answer: Upon your order, we will start to translate GB/T 29888-2013_English as soon as possible, and keep you informed of the progress. The lead time is typically 1 ~ 3 working days. 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