GB/T 28078-2011 English PDF

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GB/T 28078-2011: Detection and identification of Xanthomonas oryzae pv. oryzae (Ishiyama) Swings et al., Xanthomonas oryzae pv. oryzicola (Fang et al.) Swings et al.
Status: Valid

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GB/T 28078-2011	509	Add to Cart	4 days	Detection and identification of Xanthomonas oryzae pv. oryzae (Ishiyama) Swings et al., Xanthomonas oryzae pv. oryzicola (Fang et al.) Swings et al.	Valid

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Basic data

Standard ID: GB/T 28078-2011 (GB/T28078-2011)
Description (Translated English): Detection and identification of Xanthomonas oryzae pv. oryzae (Ishiyama) Swings et al., Xanthomonas oryzae pv. oryzicola (Fang et al.) Swings et al.
Sector / Industry: National Standard (Recommended)
Classification of Chinese Standard: B16
Classification of International Standard: 65.020.01
Word Count Estimation: 22,264
Date of Issue: 2011-12-30
Date of Implementation: 2012-06-01
Quoted Standard: GB 15569-1995; ISTA International Seed testing protocols
Regulation (derived from): Announcement of Newly Approved National Standards No. 23 of 2011
Issuing agency(ies): General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China, Standardization Administration of the People's Republic of China
Summary: This standard specifies the paddy rice seeds and other materials oryzae Xanthomonas oryxae pv. oryxae (Xoo) and rice bacterial leaf spot pathogen Xanthomonas oryzae pv. oryzicola (Xcola) the quarantine and identification of plant morphological characteristics, physiological biochemical characteristics, molecular biology and enzyme immunoassay techniques as the basis for a clear field observation, isolation and identification, sample preservation methods. This standard applies to materials and related environments Rice oryzae, rice bacterial leaf spot pathogen detection.

GB/T 28078-2011: Detection and identification of Xanthomonas oryzae pv. oryzae (Ishiyama) Swings et al., Xanthomonas oryzae pv. oryzicola (Fang et al.) Swings et al.

---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Detection and identification of Xanthomonas oryzae pv.oryzae (Ishiyama) Swings et al., Xanthomonas oryzae pv.oryzicola (Fang et al.) Swings et al. ICS 65.020.01 B16 National Standards of People's Republic of China Xanthomonas oryzae, rice bacterial leaf spot pathogen Quarantine and identification methods DetectionandidentificationofXanthomonasoryzaepv.oryzae (Ishiyama) Swingsetal., Xanthomonasoryzaepv.oryzicola (Fangetal.) Swingsetal. Issued on. 2011-12-30 2012-06-01 implementation Administration of Quality Supervision, Inspection and Quarantine of People's Republic of China Standardization Administration of China released

Foreword

This standard was drafted in accordance with GB/T 1.1-2009 given rules. This standard by the National Standardization Technical Committee of Plant Quarantine (SAC/TC271) and focal points. This standard was drafted. Exit Inspection and Quarantine of Hunan People's Republic of China, Chinese Academy of Inspection and Quarantine, the People's Republic of China Exit Inspection and Quarantine and the State of Xiamen. The main drafters of this standard. Zhu Jinguo, Zhao Wenjun, Tanglian Fei, Zhu Shuifang, Mo Jin, Zi Peng, Chen Yun, Zhong Wenying. Xanthomonas oryzae, rice bacterial leaf spot pathogen Quarantine and identification methods

1 Scope

This standard specifies the paddy rice seeds and other materials oryzae Xanthomonasoryzaepv.oryzae (Xoo) And rice bacterial leaf spot pathogen Xanthomonasoryzaepv.oryzicola (Xcola) quarantine to identify morphological characteristics of a plant, raw Physical and biochemical characteristics, molecular biology and enzyme immunoassay techniques as the basis for a clear field observation, identification, sample preservation method of separation. This standard applies to rice and related material environment oryzae, rice bacterial leaf spot pathogen detection.

2 Normative references

The following documents for the application of this document is essential. For dated references, only the dated version suitable for use herein Member. For undated references, the latest edition (including any amendments) applies to this document. GB 15569-1995 transporting agricultural plant quarantine rules Inspection of ISTA International Seed

3 PRINCIPLE OF THE METHOD

According to the morphological characteristics of rice plants were field observation, and the use of isolation and culture, molecular biology and enzyme-linked immunological screening, physiological Biochemical identification and determination of the pathogenicity of Xanthomonas oryzae on plant material, rice bacterial leaf spot pathogen determination.

4 Equipment and Materials

4.1 freezing high-speed centrifuge. speed ≤15000r/min. 4.2 PCR amplification. 4.3 incubator. 28 ℃ ± 1 ℃. 4.4 Microscope. Lens was 10 × ~ 100 ×. 4.5 Balance. Precision 0.001g. 4.6 autoclave. 4.7 homogenizer. Speed \u200b\u200b4000r/min ~ 8000r/min. 4.8 Adjustable Pipette. 0.2μL ~ 1μL, 1μL ~ 10μL, 10μL ~ 100μL, 100μL ~ 1000μL. 4.9 appliance. sterilized tweezers, scissors, weighing spoon. 4.10 straw. 1mL, 10mL. 4.11 sterilized dishes. diameter 90mm, glass or plastic disposable plates. 4.12 flask. 100mL.

5 medium and reagents

5.1 SPA medium. see A.1. ......

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