GB/T 28068-2011 English PDFUS$419.00 ยท In stock
Delivery: <= 3 days. True-PDF full-copy in English will be manually translated and delivered via email. GB/T 28068-2011: Detection of Xanthomonas citri subsp. citri using the real-time fluorescent PCR Status: Valid
Basic dataStandard ID: GB/T 28068-2011 (GB/T28068-2011)Description (Translated English): Detection of Xanthomonas citri subsp. citri using the real-time fluorescent PCR Sector / Industry: National Standard (Recommended) Classification of Chinese Standard: B16 Classification of International Standard: 65.020.01 Word Count Estimation: 18,184 Date of Issue: 2011-12-30 Date of Implementation: 2012-06-01 Quoted Standard: GB 5040; G/T 19495.2 Regulation (derived from): Announcement of Newly Approved National Standards No. 23 of 2011 Issuing agency(ies): General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China, Standardization Administration of the People's Republic of China Summary: This standard specifies the citrus canker (Xanthomonas citri subsp. citri, Xcc) real-time PCR detection of sample preparation, detection technology, operating methods and results criteria. This standard applies to citrus canker citrus plant material in real-time PCR detection and disease identification. GB/T 28068-2011: Detection of Xanthomonas citri subsp. citri using the real-time fluorescent PCR---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.Detection of Xanthomonas citri subsp.citri using the real-time fluorescent PCR ICS 65.020.01 B16 National Standards of People's Republic of China Citrus canker real-time PCR detection method fluorescentPCR Issued on. 2011-12-30 2012-06-01 implementation Administration of Quality Supervision, Inspection and Quarantine of People's Republic of China Standardization Administration of China released ForewordThis standard was drafted in accordance with GB/T 1.1-2009 given rules. This standard by the National Standardization Technical Committee of Plant Quarantine (SAC/TC271) and focal points. This standard was drafted. Chongqing University, Department of Agriculture Agricultural Technology Extension Service Center, People's Republic of China Beijing Entry-Exit Inspection and Quarantine Bureau. The main drafters of this standard. Wang Zhongkang, Yan Youping, Wang Fuxiang, high-Na Wen, Xia Yu first, Li Zhengguo, Cao Yueqing. Citrus canker real-time PCR detection method1 ScopeThis standard specifies the citrus canker (Xanthomonascitrisubsp.citri, Xcc) of real-time PCR detection system samples Equipment, detection technology, methods of operation and results criteria. This standard applies to citrus canker citrus plant material in real-time PCR pathogen detection and disease identification.2 Normative referencesThe following documents for the application of this document is essential. For dated references, only the dated version suitable for use herein Member. For undated references, the latest edition (including any amendments) applies to this document. GB 5040 citrus nursery stock quarantine rules GB/T 19495.2 technical requirements for GMO testing laboratories3 Terms and DefinitionsThe following terms and definitions apply to this document. 3.1 Citrus canker citruscankerdisease Citrus canker caused by the domestic and foreign citrus quarantine bacterial diseases. Characterized by symptoms of orange leaves, branches and fruit surface Ulcers appear raised corky lesions, resulting in citrus and deciduous fruit drop, weak grower seriously affected citrus production and fruit quality of appearance. 3.2 Citrus canker Xanthomonascitrisubsp.citri Refers to cause pathogenic bacteria in Asian citrus canker, Xanthomonas is Xanthomonas Citrus subspecies Combination citrus (Schaad2006; Young2008). 3.3 Real-time fluorescence PCR real-timefluorescentPCR Real time polymerase chain reaction method for amplifying a trace amount of a particular DNA fragment in vitro method, since the fluorescence during amplification Release of substances and can be real-time detection and rapid and sensitive detection of the presence of template DNA. 3.4 Primer amplification primer Synthetic oligonucleotide sequence, the sequence of the target DNA sequence to be amplified in the same period, for guiding the DNA in vitro Amplification. 3.5 Amplification template templet Target sequence to be amplified in vitro DNA amplification used. 3.6 Ct values \u200b\u200bcyclethresholdvalue Real time PCR reaction, each reaction tube fluorescent signal reaches a threshold value is set when the number of cycles experienced. ......Tips & Frequently Asked Questions:Question 1: How long will the true-PDF of GB/T 28068-2011_English be delivered?Answer: Upon your order, we will start to translate GB/T 28068-2011_English as soon as possible, and keep you informed of the progress. 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