GB/T 27982-2011 English PDF

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GB/T 27982-2011: Diagnostic techniques for peste des petits ruminants
Status: Valid

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Basic data

Standard ID: GB/T 27982-2011 (GB/T27982-2011)
Description (Translated English): Diagnostic techniques for peste des petits ruminants
Sector / Industry: National Standard (Recommended)
Classification of Chinese Standard: B41
Classification of International Standard: 11.220
Word Count Estimation: 14,180
Date of Issue: 2011-12-30
Date of Implementation: 2012-06-01
Quoted Standard: GB/T 6682; GB 19489-2008
Regulation (derived from): Announcement of Newly Approved National Standards No. 23 of 2011
Issuing agency(ies): General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China, Standardization Administration of the People's Republic of China
Summary: This standard specifies the peste des petits ruminants clinical diagnosis and laboratory diagnosis of technical requirements. This standard applies to the diagnosis of peste des petits ruminants.

GB/T 27982-2011: Diagnostic techniques for peste des petits ruminants

---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Diagnostic techniques for peste des petits ruminants ICS 11.220 B41 National Standards of People's Republic of China Peste des petits ruminants Diagnosis Issued on. 2011-12-30 2012-06-01 implementation Administration of Quality Supervision, Inspection and Quarantine of People's Republic of China Standardization Administration of China released

Foreword

This standard was drafted in accordance with GB/T 1.1-2009 given rules. The standard proposed by the People's Republic of China Ministry of Agriculture. This standard by the National Standardization Technical Committee Animal Epidemic Prevention (SAC/TC181) centralized. This standard was drafted. Ministry of Agriculture Key Laboratory of Tropical and Subtropical Animal Virology, China Animal Health and Epidemiology Center, China Inspection and Quarantine Science Research Institute. The main drafters of this standard. Package quiet month, Wu Xiaodong, Wang Zhiliang, Li Lin, Liuchun Ju, Wang Qinghua, Zhao Wenji, Zou Yanli, Zhengdong Xia, Xu Tiangang, Li Jinming, John Doe Yao, Zhang Yue, Lin Xiangmei, Wushao Qiang, Han Xueqing. Peste des petits ruminants Diagnosis

1 Scope

This standard specifies the technical requirements for clinical and laboratory diagnosis of peste des petits ruminants. This standard applies to the diagnosis of PPR.

2 Normative references

The following documents for the application of this document is essential. For dated references, only the dated version suitable for use herein Member. For undated references, the latest edition (including any amendments) applies to this document. Laboratory use specifications and test methods GB/T 6682 Analysis GB 19489-2008 General requirements for laboratory biosafety

3 Terms and Definitions

The following terms and definitions apply to this document. 3.1 Fluorescence quantitative reverse transcription - polymerase chain reaction realtimequantitativereversetranscriptionpolimerasechainre- action Fluorescence quantitative RT-PCR reactions Join fluorophore in the RT-PCR reaction system, real-time monitoring of fluorescence signals accumulated throughout the RT-PCR process, and finally through A standard curve of the unknown template for quantitative analysis. 3.2 Fluorescence threshold threshold 15 cycles before fluorescence signal of fluorescence quantitative RT-PCR reactions as the default background fluorescence signal, fluorescence threshold is 10 times the standard deviation of 3 to 15 cycles of the fluorescence signal. 3.3 Ct values \u200b\u200bCtvalue When the number of cycles for each reaction tube fluorescent signal reaches the threshold set by the fluorescence experienced.

4 biosecurity measures

PPR small laboratory detection, such as virus isolation, serum treatment, in accordance with GB 19489-2008.

5 clinical diagnosis

5.1 clinical symptoms 5.1.1 abrupt onset of fever, the first two days - the first three days the body temperature of 40 ℃ ~ 42 ℃ peak. Fever lasts about 3d, Bing Yang died and more concentrated in the hair Hot late. ......

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