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GB/T 27981-2011 English PDF

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GB/T 27981-2011: Real-time PCR method for the detection of infectious bovine rhinotracheitis virus
Status: Valid
Standard IDUSDBUY PDFLead-DaysStandard Title (Description)Status
GB/T 27981-2011229 Add to Cart 3 days Real-time PCR method for the detection of infectious bovine rhinotracheitis virus Valid

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Basic data

Standard ID: GB/T 27981-2011 (GB/T27981-2011)
Description (Translated English): Real-time PCR method for the detection of infectious bovine rhinotracheitis virus
Sector / Industry: National Standard (Recommended)
Classification of Chinese Standard: B41
Classification of International Standard: 11.220
Word Count Estimation: 10,155
Date of Issue: 2011-12-30
Date of Implementation: 2012-06-01
Quoted Standard: GB/T 6682
Regulation (derived from): Announcement of Newly Approved National Standards No. 23 of 2011
Issuing agency(ies): General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China, Standardization Administration of the People's Republic of China
Summary: This standard specifies the infectious bovine rhinotracheitis virus (bovine herpes virus type I, BoHV-1) real-time PCR detection method of the technical requirements. This standard applies to quick detection of bovine blood samples, swabs, semen, animal tissue (mucosa, liver, spleen, kidney, lymph nodes, placenta, etc.) and other cell culture samples BoHV-1 infection.

GB/T 27981-2011: Real-time PCR method for the detection of infectious bovine rhinotracheitis virus


---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Real-time PCR method for the detection of infectious bovine rhinotracheitis virus ICS 11.220 B41 National Standards of People's Republic of China Real-time infectious bovine rhinotracheitis virus PCR assay method Issued on. 2011-12-30 2012-06-01 implementation Administration of Quality Supervision, Inspection and Quarantine of People's Republic of China Standardization Administration of China released

Foreword

This standard was drafted in accordance with GB/T 1.1-2009 given rules. This standard reference OIE's "terrestrial animal diagnostic tests and vaccines Handbook" (2008 Edition) on infectious bovine rhinotracheitis/Cattle Biography Stained purulent vulvovaginitis (Chapter2.4.13) content. This standard by the National Standardization Technical Committee Animal Epidemic Prevention (SAC/TC181) centralized. This standard was drafted. People's Republic of China Guangdong CIQ, People's Republic of China Jiangsu Entry-Exit Inspection and Quarantine Bureau. The main drafters of this standard. Chen Ru, Liu Zhongyong, Lin Zhixiong, Luo Qiong, ZENG Bi Jian, such as Su Xu, Jiang Yan, Wu Xiaowei. Real-time infectious bovine rhinotracheitis virus PCR assay method

1 Scope

This standard specifies the infectious bovine rhinotracheitis virus (bovine herpesvirus type I, BoHV-1) real-time PCR technology to detect fluorescence method Claim. This standard applies to quick detection of bovine blood samples, swabs, semen, animal tissues (mucosa, liver, spleen, kidney, lymph node, placenta, etc.) and cell Cultures and other samples BoHV-1 infection.

2 Normative references

The following documents for the application of this document is essential. For dated references, only the dated version suitable for use herein Member. For undated references, the latest edition (including any amendments) applies to this document. Laboratory use specifications and test methods GB/T 6682 Analysis World Organisation for Animal Health (OIE) "Terrestrial animal diagnostic tests and vaccines Standards Manual" (2008 edition) for the detection of frozen bovine semen Real-time PCR method BoHV-1 virus

3 Abbreviations

The following abbreviations apply to this document. BoHV-1. bovineherpesvirustype1, bovine herpes virus type I. Fluorescent signal cycle number reaches a set threshold when experienced. Ct value. DNA. deoxyribonucleicacid, DNA. DTT. DL-dithiothreitol, dithiothreitol. EDTA. ethylenediaminetetraaceticacid, ethylenediaminetetraacetic acid. IBR. bovineinfectiousrhinotracheitis, infectious bovine rhinotracheitis. PBS. phosphatebuffersolution, phosphate buffered saline. PCR. polymerasechainreaction, polymerase chain reaction. SDS. sodiumdodecylsulfate, sodium dodecyl sulfate. UDG enzyme. uracilDNAglycosylase, uracil DNA glycosylase.

4 installations and equipment

4.1 fluorescent PCR instrument. 4.2 desktop refrigerated centrifuge. Adjustable micropipette 4.3. Specifications 10μL, 100μL, 200μL, 1mL. 4.4 with a trace filter tip. Specifications 10μL, 100μL, 200μL, 1mL. 4.5 PCR reaction tube optics. 4.6 microcentrifuge tube. 4.7 Vortex device. 4.8 incubator. 4.9 Refrigerator. Specification 2 ℃ ~ 8 ℃, -20 ℃, -80 ℃.
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