GB/T 23495-2009 English PDF

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GB/T 23495-2009: Determination of benzoic acid, sorbic acid and saccharin sodium in foods -- High performance liquid chromatography method
Status: Obsolete

Standard ID	USD	BUY PDF	Lead-Days	Standard Title (Description)	Status
GB/T 23495-2009	239	Add to Cart	3 days	Determination of benzoic acid, sorbic acid and saccharin sodium in foods -- High performance liquid chromatography method	Obsolete

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Basic data

Standard ID: GB/T 23495-2009 (GB/T23495-2009)
Description (Translated English): Determination of benzoic acid, sorbic acid and saccharin sodium in foods -- High performance liquid chromatography method
Sector / Industry: National Standard (Recommended)
Classification of Chinese Standard: X04
Classification of International Standard: 67.040
Word Count Estimation: 6,665
Date of Issue: 2009-04-27
Date of Implementation: 2009-08-01
Quoted Standard: GB/T 6682
Regulation (derived from): National Health and Family Planning Commission Notice No. 17 of 2016
Issuing agency(ies): General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China, Standardization Administration of the People's Republic of China
Summary: This standard specifies the acid in food, performance liquid chromatographic method sorbic acid and saccharin sodium content. This standard applies to foods benzoic acid, determination of sorbic acid and saccharin sodium content. The detection limit of this standard: For solid food, benzoic acid, sorbic acid, saccharin sodium detection limit of 1. 8mg/kg, 1. 2mg/kg, 3. 0mg/kg.

GB/T 23495-2009: Determination of benzoic acid, sorbic acid and saccharin sodium in foods -- High performance liquid chromatography method

---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Determination of benzoic acid, sorbic acid and saccharin sodium in foods. High performance liquid chromatography method ICS 67.040 X04 National Standards of People's Republic of China Determination of benzoic acid, sorbic acid and saccharin sodium High Performance Liquid Chromatography Posted 2009-04-27 2009-08-01 implementation Administration of Quality Supervision, Inspection and Quarantine of People's Republic of China Standardization Administration of China released

Foreword

Appendix A of this standard is an informative annex. This standard is proposed and managed by China National Institute of Standardization. This standard was drafted. Chongqing Institute of Metrology and Quality Inspection, Shanxi Province, Food Quality Supervision and Inspection Center. The main drafters of this standard. Chen Shiqi, Gong strong, Zhao Bo, Feng Xiaobin, Li Genrong, Wu Rui, He Jun, Gong Yingkun, bow CONCERETE Tu Dawei. Determination of benzoic acid, sorbic acid and saccharin sodium High Performance Liquid Chromatography

1 Scope

This standard specifies the food of benzoic acid, sorbic acid and high performance liquid chromatographic method saccharin sodium content. This standard applies to foods benzoic acid, sorbic acid and saccharin sodium content was measured. The detection limit of this standard. were 1.8mg/kg for solid foods, benzoic acid, sorbic acid, sodium saccharin detection limits, 1.2mg/kg, 3.0mg/kg.

2 Normative references

The following documents contain provisions which, through reference in this standard, constitute provisions of this standard. For dated references, subsequent Amendments (not including errata content) or revisions do not apply to this standard, however, encourage the parties to the agreement are based on research Whether the latest versions of these documents. For undated reference documents, the latest versions apply to this standard. GB/T 6682 analytical laboratory use specifications and test methods (GB/T 6682-2008, ISO 3696. 1987, MOD) Principle 3 After the different samples were extracted and the extract was filtered by reverse phase high performance liquid chromatography assay based on retention time qualitative, external standard peak area Quantified.

4 Reagents and materials

Unless otherwise indicated, the reagents used were of analytical grade, test water line with GB/T 6682 requirements. 4.1 Methanol. chromatographically pure. 4.2 ammonium acetate solution. Weigh 1.54g ammonium acetate, dissolved in water and diluted to 1000mL, microporous membrane (4.10) and filtered. 4.3 potassium ferrocyanide solution. Weigh 106g of potassium ferrocyanide [K4Fe (CN) 6 · 3H2O] add water to 1000mL. 4.4 zinc acetate solution. Weigh 220g zinc acetate [Zn (CH3COO) 2 · 2H2O] was dissolved in a small amount of water, 30mL of glacial acetic acid was added, water was added Diluted to 1000mL. 4.5 ammonia water (1 + 1). ammonia mixed with an equal volume of water. 4.6 n-hexane. 4.7 pH4.4 acetate buffer solution. a) sodium acetate solution. Weigh 6.80g of sodium acetate (CH3COONa · 3H2O), dissolved in water and dilute to 1000mL. b) acetic acid solution. Take 4.3mL glacial acetic acid, diluted with water to 1000mL. The above two solutions were mixed in a volume ratio 37.63, that was pH4.4 acetate buffer solution. 4.8 pH7.2 phosphate buffer solution. a) Weigh 23.88g disodium hydrogen phosphate (Na2HPO4 · 12H2O), dissolved in water and dilute to 1000mL. b) weighed 9.07g potassium dihydrogen phosphate (KH2PO4), dissolved in water and dilute to 1000mL. The above two phosphate solution by volume mixing ratio of 7/3, that was pH7.2 phosphate buffer solution. 4.9 Preparation of standard solution. a) benzoic acid standard stock solution. Weigh accurately 0.2360g sodium benzoate, dissolved in water and dilute to 200mL. This solution per milliliter Benzoic acid containing the equivalent of 1.00mg. ......

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