GB/T 23212-2008 English PDF

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GB/T 23212-2008: Determination of aflatoxin B1, B2, G1, G2, M1, M2 content in milk and milk powder -- HPLC-fluorescence detection method
Status: Obsolete

Standard ID	USD	BUY PDF	Lead-Days	Standard Title (Description)	Status
GB/T 23212-2008	359	Add to Cart	3 days	Determination of aflatoxin B1, B2, G1, G2, M1, M2 content in milk and milk powder -- HPLC-fluorescence detection method	Obsolete

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Basic data

Standard ID: GB/T 23212-2008 (GB/T23212-2008)
Description (Translated English): Determination of aflatoxin B1, B2, G1, G2, M1, M2 content in milk and milk powder -- HPLC-fluorescence detection method
Sector / Industry: National Standard (Recommended)
Classification of Chinese Standard: X04
Classification of International Standard: 67.050
Word Count Estimation: 9,997
Date of Issue: 2008-12-31
Date of Implementation: 2009-05-01
Quoted Standard: GB/T 6379.1; GB/T 6379.2; GB/T 6682
Regulation (derived from): National Standard Approval Announcement 2008 No.27 (Total No.140)
Issuing agency(ies): General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China, Standardization Administration of the People's Republic of China
Summary: This standard specifies the milk, milk aflatoxin B1, B2, G1, G2, M1, M2 content of liquid chromatography-fluorescence detection. This standard applies to milk, milk aflatoxin B1, B2, G1, G2, M1, determination M2 content. The detection limit of the standard methods: Milk: Aflatoxin B1 is 0. 002��g/kg, aflatoxin B2 is 0. 001��g/kg, aflatoxin G1, G2 is 0. 003��g/kg, aflatoxin M1, M2 is 0. 005��g/kg, milk powder: aflatoxin B1 is 0. 02��g/kg, aflatoxin B2 is 0. 01��g/kg, aflatoxin G1, G2 is 0. 03��g/kg, aflatoxin M1, M2 is 0. 05��g/kg.

GB/T 23212-2008: Determination of aflatoxin B1, B2, G1, G2, M1, M2 content in milk and milk powder -- HPLC-fluorescence detection method

---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Determination of aflatoxin B1, B2, G1, G2, M1, M2 content in milk and milk powder. HPLC-fluorescence detection method ICS 67.050 X04 National Standards of People's Republic of China Milk and milk powder aflatoxins Determination of B1, B2, G1, G2, M1, M2's Liquid chromatography - fluorescence detection Posted 2008-12-31 2009-05-01 implementation Administration of Quality Supervision, Inspection and Quarantine of People's Republic of China Standardization Administration of China released

Foreword

The Standard Appendix A, Appendix B is an informative annex. This standard is proposed and managed by the State Administration of Quality Supervision Inspection and Quarantine of People's Republic of China. This standard was drafted. People's Republic of China Exit Inspection and Quarantine Qinhuangdao, Hebei People's Republic of China Entry-Exit Inspection and Quarantine Bureau. The main drafters of this standard. Chenrui Chun, Duan Wenzhong, Lv Hongying, Guo Chunhai, Wang Danna, Liu Lingyun, Yaochun Yi, Yangxiu Ping, Pang Guofang. Milk and milk powder aflatoxins Determination of B1, B2, G1, G2, M1, M2's Liquid chromatography - fluorescence detection

1 Scope

This standard specifies the milk, milk powder aflatoxin B1, B2, G1, G2, M1, M2 content of liquid chromatography - fluorescence detection. This standard applies to the determination of milk, milk powder aflatoxins B1, B2, G1, G2, M1, M2 content. The detection limit of the standard methods. Milk. aflatoxin B1 was 0.002μg/kg, aflatoxin B2 was 0.001μg/kg, Aflatoxin G1, G2 is 0.003μg/kg, aflatoxin M1, M2 is 0.005μg/kg; milk powder. aflatoxin B1 is 0.02μg/kg, aflatoxin B2 was 0.01μg/kg, aflatoxin G1, G2 is 0.03μg/kg, aflatoxin M1, M2 is 0.05μg/kg.

2 Normative references

The following documents contain provisions which, through reference in this standard and become the standard terms. For dated references, subsequent Amendments (not including errata content) or revisions do not apply to this standard, however, encourage the parties to the agreement are based on research Whether the latest versions of these documents. For undated reference documents, the latest versions apply to this standard. GB/T 6379.1 measurement methods and results of Accuracy (trueness and precision) - Part 1. General principles and definitions (GB/T 6379.1- 2004, ISO 5725-1.1994, IDT) GB/T 6379.2 measurement methods and results of Accuracy (trueness and precision) - Part 2. Determine the standard measurement method to repeat Of the basic methods of reproduction (GB/T 6379.2-2004, ISO 5725-2.1994, IDT) GB/T 6682 analytical laboratory use specifications and test methods (GB/T 6682-2008, ISO 3696. 1987, MOD) Principle 3 The sample was dissolved, centrifuged, filtered, when the sample by immunoaffinity column, aflatoxin and the presence of specific antibodies selective yellow Aflatoxin B1, B2, G1, G2, M1, M2 (antigen) are bonded to form an antibody - antigen complex. Methanol - acetonitrile mixed solution was eluted with fluorescence Determination of aflatoxin B1, B2, G1, G2, M1, M2 content of external standard detected by the HPLC column derivatization.

4 Reagents and materials

Unless otherwise indicated, the reagents used were of analytical grade water as a water GB/T 6682 regulations. 4.1 Methanol. chromatographically pure. 4.2 acetonitrile. chromatography. Over 4.3 bromide Bromide. 4.4 methanol - acetonitrile mixed solution (4 + 5). Take 40mL methanol and 50mL of acetonitrile was added, mixed evenly. After 4.5 column derivatization solution. Weigh 0.05g too Bromide bromide dissolved in 1000mL water to 0.45μm nylon membrane filter, 4 ℃ stored. 4.6 Aflatoxin B1 (CASNO.1162-65-8), B2 (CASNO.7220-81-7), G1 (CASNO.1165-39-5), G2 (CASNO.7241-98-7), M1 (CASNO.6795-23-9), M2 (CASNO.6885-57-0) standard substance. purity equal to or greater At 99%. ......

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