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GB/T 22260-2023 English PDF

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US$269.00 · In stock
Delivery: <= 3 days. True-PDF full-copy in English will be manually translated and delivered via email.
GB/T 22260-2023: Determination of anabolic steroids in feeds - Liquid chromatography-tandem mass spectrometry
Status: Valid

GB/T 22260: Historical versions

Standard ID	USD	BUY PDF	Lead-Days	Standard Title (Description)	Status
GB/T 22260-2023	269	Add to Cart	3 days	Determination of anabolic steroids in feeds - Liquid chromatography-tandem mass spectrometry	Valid
GB/T 22260-2008	319	Add to Cart	3 days	Determination of methyltestosterone in feeds -- High performance liquid chromatography tandem mass spectrometry	Obsolete

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Basic data

Standard ID: GB/T 22260-2023 (GB/T22260-2023)
Description (Translated English): Determination of anabolic steroids in feeds - Liquid chromatography-tandem mass spectrometry
Sector / Industry: National Standard (Recommended)
Classification of Chinese Standard: B46
Classification of International Standard: 65.120
Word Count Estimation: 14,136
Date of Issue: 2023-08-06
Date of Implementation: 2024-03-01
Older Standard (superseded by this standard): GB/T 22260-2008
Issuing agency(ies): State Administration for Market Regulation, China National Standardization Administration

GB/T 22260-2023: Determination of anabolic steroids in feeds - Liquid chromatography-tandem mass spectrometry

---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
ICS 65.120 CCSB46 National Standards of People's Republic of China Replace GB/T 22260-2008 Determination of protein anabolic hormones in feed Liquid chromatography-tandem mass spectrometry Published on 2023-08-06 2024-03-01 Implementation State Administration for Market Regulation Released by the National Standardization Administration Committee

Foreword

This document complies with the provisions of GB/T 1.1-2020 "Standardization Work Guidelines Part 1.Structure and Drafting Rules of Standardization Documents" Drafting. This document replaces GB/T 22260-2008 "Determination of methyltestosterone in feed by high performance liquid chromatography tandem mass spectrometry" and is consistent with Compared with GB/T 22260-2008, in addition to structural adjustments and editorial changes, the main technical changes are as follows. a) Increased detection limit (see Chapter 1); b) The principle has been changed (see Chapter 4, Chapter 3 of the.2008 edition); c) Changed the extraction and purification of the sample (see 8.1, 8.2, 7.1, 7.2 of the.2008 edition); d) The reference conditions for liquid chromatography and mass spectrometry have been changed (see 8.3.1 and 8.3.2, 7.3.1 and 7.3.2 of the.2008 edition); e) Changed the qualitative and quantitative requirements for liquid chromatography-tandem mass spectrometry (see 8.3.4 and 8.3.5, 7.3.3 of the.2008 edition); f) Changed the test data processing (see Chapter 9, 8.1 of the.2008 edition); g) Changed precision (see Chapter 10, Chapter 9 of the.2008 edition). Please note that some content in this document may be subject to patents. The publisher of this document assumes no responsibility for identifying patents. This document is proposed and coordinated by the National Feed Industry Standardization Technical Committee (SAC/TC76). This document was drafted by. Shanghai Animal Disease Prevention and Control Center, Feed Research Institute of the Chinese Academy of Agricultural Sciences. The main drafters of this document. Huang Shixin, Wang Bo, Gu Xu, Cao Ying, Zhang Jing, Yan Feng, Shang Jun, Wu Jianping, Jia Ming, Wang Jie, Li Junguo, Zhang Wengang and Huang Jiaying. The previous versions of this document and the documents it replaces are as follows. ---First published as GB/T 22260-2008 in.2008; ---This is the first revision. Determination of protein anabolic hormones in feed Liquid chromatography-tandem mass spectrometry

1 Scope

This document describes a method for the liquid chromatography-tandem mass spectrometry determination of protein anabolic hormones in feed. This document is applicable to testosterone, methane and alpha in compound feeds, concentrated feeds, concentrate supplements, additive premixed feeds and mixed feed additives. Basetestosterone, Boldirone, Meandrosterone, Androstenedione, Dehydroepiandrosterone, Nandrolone, Nandrolone Propionate, Stanozolol, Melengestrol Acetate, Progesterone Determination. The detection limit of methyltestosterone, stanozolol and melengestrol acetate in this document is 0.005mg/kg, and the detection limit of testosterone, bodiron, mestandrostenone and androstene is 0.005mg/kg. The detection limit of diketone, dehydroepiandrosterone, nandrolone, nandrolone propionate, and progesterone is 0.02 mg/kg; methyltestosterone, stanozolol, and melengestrol acetate The limit of quantification for ketones is 0.01mg/kg, and the determination of testosterone, bodiron, meandrosterone, androstenedione, dehydroepiandrosterone, nandrolone, nandrolone propionate, and progesterone. The amount limit is 0.05mg/kg.

2 Normative reference documents

The contents of the following documents constitute essential provisions of this document through normative references in the text. Among them, the dated quotations For undated referenced documents, only the version corresponding to that date applies to this document; for undated referenced documents, the latest version (including all amendments) applies to this document. GB/T 6682 Specifications and test methods for water used in analytical laboratories GB/T 20195 Preparation of animal feed samples

3 Terms and definitions

There are no terms or definitions to be defined in this document.

4 Principles

The protein anabolic hormones in the sample were extracted with acetonitrile, purified by the adsorbent, and then detected with a liquid chromatography-tandem mass spectrometer using the internal standard method. Quantitative.

5 reagents or materials

5.1 Water. GB/T 6682, Level 1. 5.2 Acetonitrile. chromatographically pure. 5.3 Formic acid. chromatographically pure. 5.4 50% acetonitrile solution. Take 50mL of acetonitrile (5.2), add water to dilute to 100mL, and mix well. 5.5 0.01% formic acid solution. Take 100μL of formic acid (5.3), add water to dilute to 1000mL, and mix well. 5.6 Standard stock solution (1mg/mL). Accurately weigh testosterone, methyltestosterone, bodiron, mestandrosterone, androstenedione, dehydroepiandrosterone, nodrol 0.01g each of nandrolone propionate, stanozolol, melengestrol acetate, and progesterone (accurate to 0.00001g) were placed in a 10mL brown container. ......

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Tips & Frequently Asked Questions:

Question 1: How long will the true-PDF of GB/T 22260-2023_English be delivered?

Answer: Upon your order, we will start to translate GB/T 22260-2023_English as soon as possible, and keep you informed of the progress. The lead time is typically 1 ~ 3 working days. The lengthier the document the longer the lead time.

Question 2: Can I share the purchased PDF of GB/T 22260-2023_English with my colleagues?

Answer: Yes. The purchased PDF of GB/T 22260-2023_English will be deemed to be sold to your employer/organization who actually pays for it, including your colleagues and your employer's intranet.

Question 3: Does the price include tax/VAT?

Answer: Yes. Our tax invoice, downloaded/delivered in 9 seconds, includes all tax/VAT and complies with 100+ countries' tax regulations (tax exempted in 100+ countries) -- See Avoidance of Double Taxation Agreements (DTAs): List of DTAs signed between Singapore and 100+ countries

Question 4: Do you accept my currency other than USD?

Answer: Yes. If you need your currency to be printed on the invoice, please write an email to Sales@ChineseStandard.net. In 2 working-hours, we will create a special link for you to pay in any currencies. Otherwise, follow the normal steps: Add to Cart -- Checkout -- Select your currency to pay.

Question 5: Should I purchase the latest version GB/T 22260-2023?

Answer: Yes. Unless special scenarios such as technical constraints or academic study, you should always prioritize to purchase the latest version GB/T 22260-2023 even if the enforcement date is in future. Complying with the latest version means that, by default, it also complies with all the earlier versions, technically.