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GB/T 22253-2008 English PDF

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US$239.00 · In stock
Delivery: <= 3 days. True-PDF full-copy in English will be manually translated and delivered via email.
GB/T 22253-2008: Determination of alitame in foods
Status: Obsolete

Standard ID	USD	BUY PDF	Lead-Days	Standard Title (Description)	Status
GB/T 22253-2008	239	Add to Cart	3 days	Determination of alitame in foods	Obsolete

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Basic data

Standard ID: GB/T 22253-2008 (GB/T22253-2008)
Description (Translated English): Determination of alitame in foods
Sector / Industry: National Standard (Recommended)
Classification of Chinese Standard: C53
Classification of International Standard: 67.040
Word Count Estimation: 6,649
Date of Issue: 2008-07-31
Date of Implementation: 2008-11-01
Regulation (derived from): National Standard Approval Announcement 2008 No.12 (Total No.125)
Issuing agency(ies): General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China, Standardization Administration of the People's Republic of China
Summary: This standard specifies the determination of Ali sweet foods. This standard applies to carbonated drinks, milk drinks, fruit drinks and solids concentration measurement alitame. When the sample weight of 5g, constant volume of 25mL, during injection 10��L, the detection limit was 0. 002g/kg, the limit of quantification of 0. 006g/kg, the linear range was 5. 0��g/mL ~ 100��g/mL.

GB/T 22253-2008: Determination of alitame in foods

---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Determination of alitame in foods ICS 67.040 C53 National Standards of People's Republic of China DETERMINATION alitame of Posted 2008-07-31 2008-11-01 implementation People's Republic of China Ministry of Health Standardization Administration of China released

Foreword

This standard is proposed and administered by the People's Republic of China Ministry of Health. This standard is drafted by. China Center for Disease Control Nutrition and Food Safety. Participated in the drafting of this standard. Disease Prevention and Control Center of Shandong Province, Jilin Province Disease Prevention and Control Center. The main drafters of this standard. Wang Zhu days Jiangding Guo, Yang Jin, Chen Jindong, Liu Sijie. This standard is the first release. DETERMINATION alitame of

1 Scope

This standard specifies the method for the determination of food in alitame. This standard applies to carbonated drinks, milk drinks, juice drinks and solid determination alitame concentrated. When the sample weight was 5g, constant volume volume of 25mL, when the injection 10μL, the detection limit was 0.002g/kg, the limit of quantification The linear range of 0.006g/kg, the method was 5.0μg/mL ~ 100μg/mL. Principle 2 According alitame and other characteristics soluble in water and ethanol solvent, solid beverage sample alitame with distilled water in the ultrasonic oscillation under extraction, The extract was washed with water volume; carbonated beverages in addition to carbon dioxide after the sample volume with water; milk beverages sample alitame protein was precipitated with ethanol, the + Supernatant volume of ethanol with water (2 + 1); extract the liquid chromatography separation ODSC18 reverse phase column, detection at 200nm wavelength, with Chromatographic retention time qualitative, external standard.

3 Reagents

3.1 methanol (CH3OH). chromatography. 3.2 Ethanol (CH3CH2OH). pure class distinctions. 3.3 alitame standard. Purity ≥99%. 3.4 Water (H2O). a laboratory water conductivity (25 ℃) of 0.01mS/m. 3.5 alitame stock standard solution (0.5mg/mL). Weigh 0.05g alitame (accurate to 0.0001g), placed in 100mL flask Was added and dissolved in water to volume, placed in the refrigerator for a period of 90d. 3.6 Preparation of solution series alitame standard Use. alitame standard stock solution by diluting with water to 100μg/mL, 50μg/mL, 25μg/mL, 10.0μg/mL, 5.0μg/mL using the standard solution series. Placed in the refrigerator for a period of 60d.

4 Instrument

4.1 liquid chromatograph. equipped with a diode array detector. 4.2 ultrasonic oscillator. 4.3 Centrifuge. 4000r/min. Step 5 Analysis 5.1 sample handling 5.1.1 carbonated beverages Weigh about 10g sample (accurate to 0.001g), 50 ℃ lukewarm removing carbon dioxide, water volume to 25mL ~ 50mL, mix from Heart, aqueous supernatant 0.45μm membrane filter and set aside. 5.1.2 Milk Drinks Weigh about 5g sample (accurate to 0.001g) in a centrifuge tube 50mL, 10mL of ethanol was added, cover and gently turned upside down a few Times (not shaken), standing 1min, 4000r/min centrifugal 5min, the supernatant was filtered into 25mL volumetric flask, precipitated with ethanol 5mL + Water (2 + 1) washing the combined supernatants after centrifugation, washed with ethanol + water (2 + 1) to the mark by 0.45μm organic membrane filter and set aside. ......

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Tips & Frequently Asked Questions:

Question 1: How long will the true-PDF of GB/T 22253-2008_English be delivered?

Answer: Upon your order, we will start to translate GB/T 22253-2008_English as soon as possible, and keep you informed of the progress. The lead time is typically 1 ~ 3 working days. The lengthier the document the longer the lead time.

Question 2: Can I share the purchased PDF of GB/T 22253-2008_English with my colleagues?

Answer: Yes. The purchased PDF of GB/T 22253-2008_English will be deemed to be sold to your employer/organization who actually pays for it, including your colleagues and your employer's intranet.

Question 3: Does the price include tax/VAT?

Answer: Yes. Our tax invoice, downloaded/delivered in 9 seconds, includes all tax/VAT and complies with 100+ countries' tax regulations (tax exempted in 100+ countries) -- See Avoidance of Double Taxation Agreements (DTAs): List of DTAs signed between Singapore and 100+ countries

Question 4: Do you accept my currency other than USD?

Answer: Yes. If you need your currency to be printed on the invoice, please write an email to Sales@ChineseStandard.net. In 2 working-hours, we will create a special link for you to pay in any currencies. Otherwise, follow the normal steps: Add to Cart -- Checkout -- Select your currency to pay.