GB/T 22244-2008 English PDFUS$139.00 · In stock
Delivery: <= 3 days. True-PDF full-copy in English will be manually translated and delivered via email. GB/T 22244-2008: Determination of procyanidins in health foods Status: Valid
Basic dataStandard ID: GB/T 22244-2008 (GB/T22244-2008)Description (Translated English): Determination of procyanidins in health foods Sector / Industry: National Standard (Recommended) Classification of Chinese Standard: C53 Classification of International Standard: 67.040 Word Count Estimation: 6,630 Date of Issue: 2008-07-31 Date of Implementation: 2008-11-01 Regulation (derived from): National Standard Approval Announcement 2008 No.12 (Total No.125) Issuing agency(ies): General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China, Standardization Administration of the People's Republic of China Summary: This standard specifies the determination of health food in front of anthocyanins. This standard applies to grape seed, grape skins, sea buckthorn, rosehip, blue berries, French pine bark extract as the main raw material for the manufacture of measuring health food in front of anthocyanins. This standard limit of detection: The detection limit was 1. 5 �� 10-4g/100g, quantification limit was 5. 0 �� 10-4g/100g. The linear range of 10��g/mL ~ 150��g/mL. GB/T 22244-2008: Determination of procyanidins in health foods---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.Determination of procyanidins in health foods ICS 67.040 C53 National Standards of People's Republic of China Determination of proanthocyanidins in health food Posted 2008-07-31 2008-11-01 implementation People's Republic of China Ministry of Health Standardization Administration of China released ForewordThis standard is proposed and administered by the People's Republic of China Ministry of Health. This standard is drafted by. China Center for Disease Control Nutrition and Food Safety. Participated in the drafting of this standard. Jilin Province Center for Disease Control and Prevention. The main drafters of this standard. Yang Jin, Xiao Jing, Fang calm, Wang Zhu days, Liu Hongtao. This standard is the first release. Determination of proanthocyanidins in health food1 ScopeThis standard specifies the method for determination of health food in front of anthocyanins. This standard applies to grape seed, grape skin, sea buckthorn, rosehip, blueberry, French pine bark extract as the main raw material for the manufacture of security Determination of proanthocyanidins healthy foods. The standard detection limits. the method detection limit (LOD) was 1.5 × 10-4g/100g, limit of quantification (LOQ) of 5.0 × 10-4g/100g. The linear range of 10μg/mL ~ 150μg/mL. Principle 2 Proanthocyanidins soluble in water, is the flavan-3-phenyl catechol and epicatechin connected together. Based on a sample monomeric or polymeric procyanidins Was heated under acidic conditions and catalytic iron salts, C-C bond cleavage to produce a deep red anthocyanin cyanide ion that is a given principle, the use of high Performance liquid chromatography on a C18 reverse phase column separation, at a wavelength of 525nm at the detection of the retention time qualitative, external standard, the measurement sample before Anthocyanin content.3 Reagents and materials3.1 methanol (CH3OH). AR. 3.2 methanol (CH3OH). chromatography. 3.3 n-butanol [CH3 (CH2) 2CH2OH]. AR. 3.4 hydrochloric acid (HCl). AR. 3.5 dichloromethane (CH2Cl2). AR. 3.6 isopropyl alcohol [(CH3) 2CHOH]. AR. 3.7 formic acid (HCOOH). AR. 3.8 ammonium ferric sulfate [NH4Fe (SO4) 2 · 12H2O]. AR. 3.9 Water (H2O). a laboratory water conductivity (25 ℃) of 0.01mS/m. 3.10 2% ferric ammonium sulfate [NH4Fe (SO4) 2 · 12H2O] solution. Weigh ammonium ferric sulfate 2g, with a concentration of dissolved 2mol/L hydrochloric acid, will be Volume to 100mL. 3.11 proanthocyanidins standard. Purity ≥98%. 3.12 procyanidin standard solution (1.00mg/mL). Weigh 0.01g procyanidin standard (accurate to 0.0001g), with A Alcohol (3.2) was dissolved and set the volume to 10mL brown volumetric flask, and this solution using now.4 instruments and equipment4.1 High performance liquid chromatograph. equipped with a UV detector. 4.2 ultrasonic cleaner. 4.3 Centrifuge. 4000r/min. Step 5 Analysis 5.1 Sample Processing 5.1.1 tablet. Take 20 specimens ground into powder. 5.1.2 Capsule. Take 20 capsule contents, and mix. ...... |
