GB/T 22221-2008 English PDF

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GB/T 22221-2008: Determination of fructose, glucose, sucrose, maltose, lactose in foods -- High-performance liquid chromatography
Status: Obsolete

Standard ID	USD	BUY PDF	Lead-Days	Standard Title (Description)	Status
GB/T 22221-2008	239	Add to Cart	3 days	Determination of fructose, glucose, sucrose, maltose, lactose in foods -- High-performance liquid chromatography	Obsolete

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Basic data

Standard ID: GB/T 22221-2008 (GB/T22221-2008)
Description (Translated English): Determination of fructose, glucose, sucrose, maltose, lactose in foods -- High-performance liquid chromatography
Sector / Industry: National Standard (Recommended)
Classification of Chinese Standard: X04
Classification of International Standard: 67.050
Word Count Estimation: 6,655
Date of Issue: 2008-05-16
Date of Implementation: 2008-10-01
Quoted Standard: GB/T 6682
Regulation (derived from): National Standard Approval Announcement 2008 No.10 (Total No.123)
Issuing agency(ies): General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China, Standardization Administration of the People's Republic of China
Summary: This standard specifies the determination of fructose in food by high performance liquid chromatography, lactose conditions and detailed analysis step, maltose, glucose, sucrose. This standard applies to cereals, dairy products, fruit and vegetable products, honey, sugar paddle, beverages and other foods fructose, glucose, sucrose, maltose, determination of lactose. This standard fructose, glucose, sucrose, maltose, lactose minimum detection limit of 0. 4%.

GB/T 22221-2008: Determination of fructose, glucose, sucrose, maltose, lactose in foods -- High-performance liquid chromatography

---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Determination of fructose, glucose, sucrose, maltose, lactose in foods. High-performance liquid chromatography ICS 67.050 X04 National Standards of People's Republic of China Food fructose, glucose, sucrose, Maltose, lactose HPLC Posted 2008-05-16 2008-10-01 implementation Administration of Quality Supervision, Inspection and Quarantine of People's Republic of China Standardization Administration of China released

Foreword

The standard proposed by China Institute of Metrology. This standard by the National Standardization Technical Committee on Food industry. This standard was drafted. China Institute of Metrology, Beijing Institute of Nutrition source, Beijing beautiful earth testing center. The main drafters of this standard. Jing, Liu Yufeng, Li Li, Sheng Linghui, Tang Huacheng, Fu Boqiang, Zhaomeng Bin, Shang Yanfen. Food fructose, glucose, sucrose, Maltose, lactose HPLC

1 Scope

This standard specifies the food fructose, glucose, sucrose, maltose, lactose conditions and a detailed analysis by HPLC step. This standard applies to cereals, dairy products, fruit and vegetable products, honey, syrups, drinks and other food fructose, glucose, sucrose, maltose, milk Determination of sugar. This standard fructose, glucose, sucrose, maltose, lactose lowest detection limit was 0.4%.

2 Normative references

The following documents contain provisions which, through reference in this standard and become the standard terms. For dated references, subsequent Amendments (not including errata content) or revisions do not apply to this standard, however, encourage the parties to the agreement are based on research Whether the latest versions of these documents. For undated reference documents, the latest versions apply to this standard. GB/T 6682 analytical laboratory use specifications and test methods (GB/T 6682-1992, neq ISO 3696. 1987)

3 Method summary

After precipitation samples extracted fat and protein filtration, the sample solution into the high pressure liquid chromatography, column separated by amino, external standard.

4 Reagents and solutions

Unless otherwise stated, should be used in the analysis of analytical reagent grade water as a water GB/T 6682 regulations. 4.1 acetonitrile. chromatography. 4.2 zinc acetate solution. Weigh 21.9g zinc acetate, 3mL plus glacial acetic acid dissolved in water and diluted to 100mL. 4.3 potassium ferrocyanide solution. Weigh 10.6g of potassium ferrocyanide, dissolved in water and diluted to 100mL. 4.4 petroleum ether. boiling range 30 ℃ ~ 60 ℃. 4.5 Sugar. purity ≥99%. 4.6 sugar stock standard solution. Weigh 1g (accurate to 0.1mg) after drying 2h ± fructose after 96 ℃ 2 ℃, glucose, sucrose, Maltose, lactose standards, adding about 50g dissolved in water, weighed (accurate to 0.1mg), fructose solutions were equivalent to 20mg per gram, Portugal Glucose, sucrose, maltose, lactose, seal placed 4 ℃ can be stored for a month. Sugar 4.7 standard working solution. Imbibe respectively sugar stock standard solution (4.6) 1.00mL, 2.00mL, 3.00mL, 5.00mL in 10mL volumetric flask, weighed (accurate to 0.1mg), add water to the mark, weighed (accurate to 0.1mg), respectively, equivalent to 2.0mg/g, 4.0mg/g, 6.0mg/g, 10.0mg/g concentration of the standard. 5. Apparatus Conventional laboratory equipment and the following. 5.1 High performance liquid chromatograph. with a refractive index detector or equivalent detector. 5.2 Column. amino column (4.6mm × 250mm, 5μm). 5.3 magnetic stirrer. 5.4 Centrifuge. 4000r/min. ......

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