GB/T 22220-2008 English PDFUS$239.00 · In stock
Delivery: <= 3 days. True-PDF full-copy in English will be manually translated and delivered via email. GB/T 22220-2008: Determination of cholesterol in foods -- High-performance liquid chromatography Status: Obsolete
Basic dataStandard ID: GB/T 22220-2008 (GB/T22220-2008)Description (Translated English): Determination of cholesterol in foods -- High-performance liquid chromatography Sector / Industry: National Standard (Recommended) Classification of Chinese Standard: X04 Classification of International Standard: 67.050 Word Count Estimation: 6,694 Date of Issue: 2008-05-16 Date of Implementation: 2008-10-01 Quoted Standard: GB/T 6682 Regulation (derived from): National Standard Approval Announcement 2008 No.10 (Total No.123) Issuing agency(ies): General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China, Standardization Administration of the People's Republic of China Summary: This standard specifies the HPLC conditions cholesterol foods heavy and detailed analysis steps. This standard applies to the determination of the cholesterol content of foods. This standard minimum detection limit of 2. 6 mg/100g. GB/T 22220-2008: Determination of cholesterol in foods -- High-performance liquid chromatography---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.Determination of cholesterol in foods. High-performance liquid chromatography ICS 67.050 X04 National Standards of People's Republic of China Determination of cholesterol in foods High Performance Liquid Chromatography Posted 2008-05-16 2008-10-01 implementation Administration of Quality Supervision, Inspection and Quarantine of People's Republic of China Standardization Administration of China released ForewordThe standard proposed by China Institute of Metrology. This standard by the National Standardization Technical Committee on Food industry. This standard was drafted. China Institute of Metrology, Beijing Institute of Nutrition source, Beijing beautiful earth testing center. The main drafters of this standard. Jing, Sheng Linghui, Li Li, Liu Yufeng, Tang Huacheng, Zhaomeng Bin, Shang Yanfen. Determination of cholesterol in foods High Performance Liquid Chromatography1 ScopeThis standard specifies the determination of cholesterol in food conditions and detailed analysis step HPLC. This standard applies to the determination of cholesterol in food. The standard minimum detection limit of 2.6mg/100g.2 Normative referencesThe following documents contain provisions which, through reference in this standard and become the standard terms. For dated references, subsequent Amendments (not including errata content) or revisions do not apply to this standard, however, encourage the parties to the agreement are based on research Whether the latest versions of these documents. For undated reference documents, the latest versions apply to this standard. GB/T 6682 analytical laboratory use specifications and test methods (GB/T 6682-1992, neq ISO 3696. 1987)3 Method summaryThe sample was ethanol - potassium hydroxide solution after saponification, petroleum ether and anhydrous ether mixed solution was extracted, dissolved in methanol quantitative, high-efficiency Liquid chromatograph, external standard.4 Reagents and solutionsUnless otherwise stated, should be used in the analysis of analytical reagent grade water as a water GB/T 6682 recommended. 4.1 Methanol. chromatographically pure. 4.2 ethanol. 4.3 petroleum ether. boiling range 30 ℃ ~ 60 ℃. 4.4 anhydrous ether. 4.5 over anhydrous sodium sulfate. 4.6 potassium hydroxide. 4.7 60% potassium hydroxide solution. Weigh 60g of potassium hydroxide, 40mL water are mixed and dissolved. 4.8 Cholesterol. Purity ≥99%. 4.9 standard stock solution. Weigh cholesterol standard 0.05g (precise to 0.1mg), dissolve with ethanol volume to 50mL, each solution Ml equivalent to 1mg cholesterol, seal placed 4 ℃ can be stored for six months. 4.10 standard solution. Pipette stock standard solution (4.9) 1.0mL, 2.0mL, 3.0mL, 5.0mL, with anhydrous ethanol to 10mL, formulated correspond 0.1mg/mL, 0.2mg/mL, 0.3mg/mL, 0.5mg/mL concentration of the standard. 5. Apparatus Conventional laboratory equipment and the following. 5.1 High performance liquid chromatograph. with a UV detector or equivalent detector. 5.2 Analysis of balance. a sense of the amount of 0.1mg. 5.3 Column. C18 reverse phase column (4.6mm × 150mm, 5μm). ......Tips & Frequently Asked Questions:Question 1: How long will the true-PDF of GB/T 22220-2008_English be delivered?Answer: Upon your order, we will start to translate GB/T 22220-2008_English as soon as possible, and keep you informed of the progress. The lead time is typically 1 ~ 3 working days. 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