GB/T 14924.10-2008 English PDF

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GB/T 14924.10-2008: Laboratory animal -- Determination of amino acids for formula feeds
Status: Valid

GB/T 14924.10: Historical versions

Standard ID	USD	BUY PDF	Lead-Days	Standard Title (Description)	Status
GB/T 14924.10-2008	149	Add to Cart	2 days	Laboratory animal -- Determination of amino acids for formula feeds	Valid
GB/T 14924.10-2001	199	Add to Cart	2 days	Laboratory animals -- Formula feeds determination of amino acids	Obsolete

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Basic data

Standard ID: GB/T 14924.10-2008 (GB/T14924.10-2008)
Description (Translated English): Laboratory animal -- Determination of amino acids for formula feeds
Sector / Industry: National Standard (Recommended)
Classification of Chinese Standard: B20
Classification of International Standard: 65.120
Word Count Estimation: 5,514
Date of Issue: 2008-12-03
Date of Implementation: 2009-03-01
Older Standard (superseded by this standard): GB/T 14924.10-2001
Quoted Standard: GB/T 5009.124; GB/T 15400; GB/T 18246-2000
Regulation (derived from): National Standard Approval Announcement 2008 No.19 (Total No.132)
Issuing agency(ies): General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China, Standardization Administration of the People's Republic of China
Summary: This standard specifies the method of experimental determination of amino acids in animal feed. This standard applies to animal experiments in mice, rat, rabbit, guinea pig, hamster, feed dogs and monkeys with determination and raw amino acids.

GB/T 14924.10-2008: Laboratory animal -- Determination of amino acids for formula feeds

---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Laboratory animal. Determination of amino acids for formula feeds ICS 65.120 B20 National Standards of People's Republic of China Replacing GB/T 14924.10-2001 Animal feed Determination of amino acids Posted 2008-12-03 2009-03-01 implementation Administration of Quality Supervision, Inspection and Quarantine of People's Republic of China Standardization Administration of China released

Foreword

Since the implementation of this standard replaces GB/T 14924.10-2001 "animal feed with the determination of amino acids." The main technical differences between standard compared with GB/T 14924.10-2001 as follows. a) in accordance with GB/T 20001.4-2001 "Rules for drafting standards - Part 4. Chemical analysis methods," the original text in standard The modification; b) a reference to the original standard adjustment; c) and the original standard errors were corrected. This standard by the National Standardization Technical Committee of experimental animals and focal points. This standard was drafted. the National Standardization Technical Committee of experimental animals. The main drafters. Zhang Yu, Jia Jianbin, Zhou Ruihua, Wang Zhu, Wang Guodong, Liu Yuan. This standard replaces the standards previously issued as follows. --- GB/T 14924-1994, GB/T 14924.10-2001. Animal feed Determination of amino acids

1 Scope

This standard specifies the method for the experimental determination of amino acids in animal feed. This standard applies to experimental animals such as mice, rats, rabbits, guinea pigs, hamsters, dogs and monkeys with feed and its raw materials in the determination of amino acids.

2 Normative references

The following documents contain provisions which, through reference in this standard and become the standard terms. For dated references, subsequent Amendments (not including errata content) or revisions do not apply to this standard, however, encourage the parties to the agreement are based on research Whether the latest versions of these documents. For undated reference documents, the latest versions apply to this standard. Determination of GB/T 5009.124 amino acids in food Determination of GB/T 15400 tryptophan in feed spectrophotometry 18246-2000 feed Determination of GB/T Amino Acids

3 Determination

3.1 Determination of Amino Acid in Feed with Performed in GB/T 18246-2000 or GB/T 5009.124 relevant provisions. 3.2 Determination of feed with tryptophan 3.2.1 The first method HPLC According to GB/T 18246-2000 Appendix A "Tryptophan by RP-HPLC" regulations. 3.2.2 Method II fluorospectrophotometry 3.2.2.1 Principle In alkaline hydrolysis fluid protein, only tryptophan and tyrosine fluorescence can be detected. At pH 11, the tryptophan fluorescence intensity 100 times larger than the degree of tyrosine, tyrosine and tryptophan fluorescence peak difference of 40nm or more. According to the characteristics, the alkaline hydrolysis process protein Quality, and then by measuring the natural fluorescence of tryptophan, tryptophan quantitative analysis. 3.2.2.2 Reagents Unless otherwise indicated, all reagents were of analytical grade, water was used a water. 3.2.2.2.1 soluble starch. 3.2.2.2.2 sodium hydroxide (NaOH). 3.2.2.2.3 sodium hydroxide solution (5mol/L) containing soluble starch. soluble starch 20g of sodium hydroxide solution (5g/L) was dissolved Solutions and set the volume to 100mL. Pro preparation before use. 3.2.2.2.4 urea solution (4mol/L, pH11). 3.2.2.2.5 hydrochloric acid solution (1 + 1). 1 part of concentrated hydrochloric acid (excellent pure) plus 1 part by weight of distilled water. 3.2.2.2.6 bromothymol blue solution (0.5g/L). 0.1g bromothymol blue, plus 0.1mol/L sodium hydroxide solution 1.6mL solution Solution, add water to 200mL. 3.2.2.2.7 high purity nitrogen (content 99.99%). 3.2.2.2.8 octanol toluene solution. Take 1mL octanol mixed with 99mL toluene. 3.2.2.2.9 tryptophan standard stock solution (1.00mg/mL). Weigh accurately tryptophan 100.0mg, with 0.005mol/L sodium hydroxide ......

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