GB/T 12763.6-2007 English PDF

Standard ID	USD	BUY PDF	Lead-Days	Standard Title (Description)	Status
GB/T 12763.6-2007	RFQ	ASK	3 days	Specifications for oceanographic survey -- Part 6: Marine biological survey	Valid
GB/T 12763.6-1991	RFQ	ASK	3 days	The specification for oceanographic survey--Marine bioligical survey	Obsolete

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Basic data

Standard ID: GB/T 12763.6-2007 (GB/T12763.6-2007)
Description (Translated English): Specifications for oceanographic survey -- Part 6: Marine biological survey
Sector / Industry: National Standard (Recommended)
Classification of Chinese Standard: A45
Classification of International Standard: 07.060
Word Count Estimation: 169,147
Date of Issue: 2007-08-13
Date of Implementation: 2008-02-01
Older Standard (superseded by this standard): GB/T 12763.6-1991
Quoted Standard: GB/T 14014; GB/T 12763.1; GB/T 12763.7; GB 17378.7
Regulation (derived from): China Announcement of Newly Approved National Standards No. 8, 2007 (No. 108 overall)
Issuing agency(ies): General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China, Standardization Administration of the People's Republic of China
Summary: This standard specifies the general requirements of marine biological investigations, technical requirements and investigation (measured) elements, sampling, sample analysis and data compilation of the basic requirements and methods. This section applies to the basic elements of the marine environment in marine survey survey.

GB/T 12763.6-2007: Specifications for oceanographic survey -- Part 6: Marine biological survey

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Specifications for oceanographic survey Part 6. Marine biological survey ICS 07.060 A45 National Standards of People's Republic of China Replacing GB/T 12763.6-1991 Oceanographic survey Part 6. Marine Biological Survey Posted 2007-08-13 2008-02-01 implementation Administration of Quality Supervision, Inspection and Quarantine of People's Republic of China Standardization Administration of China released

Table of Contents

Preface Ⅶ 1 Scope 1 2 Normative references 1 3 Terms and definitions 3 4 General provisions 4.1 Technical Design 3 4.2 Survey Requirements 3 4.3 Investigation and analysis equipment 4 4.4 Sample 4 4.5 Sample Analysis 5 4.6 data compilation and report preparation 5 5 Determination of chlorophyll, primary productivity and new production of 6 5.1 Determination of technical requirements and features 6 5.2 seawater phytoplankton pigments measuring 6 5.3 marine primary productivity (14C tracer method) 10 5.4 size of chlorophyll a and primary productivity grade determination 11 5.5 new marine productivity (15N tracer method) 12 5.6 data compilation 14 18 6 microorganisms survey 6.1 Technical requirements and investigation elements 18 6.2 Sampling 19 19 6.3 Sample Analysis 6.4 data compilation 29 7 pico, micro and small plankton surveys 30 7.1 Technical requirements and investigation elements 30 7.2 Sampling 30 7.3 Sample Analysis 32 7.4 data compilation 33 8 large and medium-sized plankton survey 34 8.1 Technical requirements and investigation elements 34 8.2 Sampling 35 36 8.3 Sample Analysis 8.4 data compilation 37 9 38 ICHTHYOPLANKTON survey 9.1 Technical requirements and investigation elements 38 9.2 Sampling 38 40 9.3 Sample Analysis 9.4 data compilation 40 10 large benthic surveys 41 10.1 Technical requirements and investigation elements 41 10.2 Sampling 41 43 10.3 Sample Analysis 10.4 data compilation 44 11 meiobenthos survey 44 11.1 Technical requirements and investigation elements 44 11.2 Sampling 45 46 11.3 Sample Analysis 11.4 data compilation 48 Between 12 intertidal Biological Survey 48 12.1 Technical requirements and investigation elements 48 12.2 Sampling 49 51 12.3 Sample Analysis 12.4 data compilation 51 13 52 Survey of fouling organisms 13.1 Technical requirements and investigation elements 52 13.2 Sampling 52 54 13.3 Sample Analysis 13.4 data compilation 55 14 nekton surveys 56 14.1 Technical requirements and investigation elements 56 14.2 Sampling 57 58 14.3 Sample Analysis 14.4 data compilation 61 Appendix A (normative) microorganism most probable number (MPN) retrieval table 63 and counting Appendix B (normative) sample number plankton biomass measurement, counting fish and plankton nets 66 Annex C (normative) macrofauna fixative preparation, sample number and underwater photographic and video 71 Annex D (normative) meiobenthos sample separation, and several computing equipment 73 Appendix E (normative) intertidal biological surveys and several tidal measurement apparatus of FIG. 79 Annex F (normative) swimming animal gonad maturity, feeding intensity, fat content and trawl type 83 Annex G (informative) acoustic surveys and fishery resources assessment (OPTIONAL) 89 Appendix H (informative) Marine Biological Survey Sampling and analysis record sheet format 98 References 158 Figure B. 1 pair of drum net Figure 69 Figure B. 2 North Pacific Network 70 Figure D. 1 meiobenthos stars like structure government side schematic view and the lateral view 76 Figure D. 2 Schematic diagram of washing sandy samples closure 76 Figure D. 3 small ice Separating biological schematic 77 Figure D. 4 different size conversion factor of 77 copepods Figure D. 5 divers carry matching scuba (SCUBA) devices such as schematic 78 Figure E. 1 tide level curve graphically curve 80 Figure E. 2 direct measurement curve 80 Figure E. 3a) covering an area of 80 count box Figure E. 3b) Rock quantitative sampling frame 80 Figure E. 4 beach quantitative sampling frame 81 Figure E. 5 whirlpool sorter 81 Figure E. 6 sieved 82 Figure F. 1 pair of winged boat bottom trawl Type A single capsule 86 Figure F. Two pairs of winged ship bottom trawl single capsule type B 87 Figure F. 3 single-winged single boat trawl bag 88 Figure G. 1 bottom trawl survey dedicated FIG. 95 Figure G. 2 survey dedicated Water Trawl nets 96 Figure G. 3 acoustic instrument calibration standard target schematic 97 suspension Table 1 water harvesting level 3 Table 2 HPLC gradient elution 10 Table 3 pico, micro and small plankton sampling aqueous layer vertical segment 30 Table 4 micro and small plankton nets specifications and application objects 31 Table 5 medium-sized plankton sampling aqueous layer vertical segment 34 Table 6 medium-sized plankton nets specifications and application objects 35 Table 7 fish and plankton nets specifications Applicable 39 Table 8 test plate type, size and quantity 53 Table A. 1 most probable number (MPN) counting (15) the number of bacteria retrieval table 63 Table B. 1 pair of drum configuration and network specifications 69 Table B. 2 North Pacific network configuration and specifications 70 Table D. 1 conversion factor meiobenthos table 74 Table F. 1 pair of winged unicystic boat bottom trawl nets type B, paragraph 7 to paragraph 24 of the knitting Size 87 Table G. 1 fish target strength and length relationship between the reference value of 94 Table H. 1 chlorophyll sampling record sheet 98 Table H. 2 chlorophyll (extracted fluorescence) measurement record table 99 Table H. 3 chlorophyll (Spectrophotometric Method) record table 100 Table H. 4 chlorophyll (HPLC) Determination record table 101 Table H. 5 primary productivity sampling, filtering, measurement recording table 102 Table H. 6 new production sampling, filtering, measurement recording table 103 Table H. 7 microbial sampling site record table 104 Table H. 8 marine waters viral, bacterial count of the number of direct recording table 105 Table H. 9 bacterial cell sizer record table 106 Table H. 10 water samples, soil samples and counting microbial culture isolates record table 107 Table H. 11 water samples bacterial productivity record table 108 Table H. 12 different bacteria in water samples Determination of record feed table 109 Table H. 13 samples of bacteria most probable number (MPN) record table 110 Table H. 14 sea plankton sample record table 111 Table H. 15 vertical stratification of plankton trawl sampling record table 112 Table H. 16 plankton samples registration table 113 Table H. 17 pico-cell photosynthetic plankton record number table 114 Table H. 18 micro and small plankton specimen count the number of records table 115 Table H. 19 water harvesting plankton count the number of sample record table 116 Table H. 20 phytoplankton (cysts) cell record table 117 Table H. 21 zooplankton volume fraction measurement record table 118 Table H. 22 zooplankton biomass wet weight measurement record table 119 Table H. 23 zooplankton biomass dry weight measurement record table 120 Table H. 24 zooplankton individual record count table 121 Table H. 25 Noctiluca individual record count table 122 Table H. 26 fish at sea plankton collected record table 123 Table H. 27 124 ICHTHYOPLANKTON specimens registration form Table H. 28 ICHTHYOPLANKTON record count table 125 Table H. 29 Number of fish plankton tables 126 Table H. 30 large sea benthic sampling record table 127 Table H. 31 large-scale quantitative analysis of benthic record table 128 Table H. Qualitative analysis of 32 large benthic record table 129 Table H. 33 large-scale quantitative analysis of benthic species distribution record table 130 Table H. Qualitative analysis of 34 large benthic species distribution record table 131 Table H. 35 meiobenthos sea sampling record table 132 Table H. 36 meiobenthos quantitative analysis recording table 1133 Table H. 37 meiobenthos quantitative analysis log table 2134 Table H. 38 meiobenthos qualitative analysis table 135 records Table H. Room 39 intertidal biological wild collection record table 136 Table H. 40 intertidal zone between quantitative analysis record table 137 Table H. 41 intertidal zone between the qualitative analysis record table 138 Table H. Room 42 intertidal species distribution table 139 Table H. Intertidal zone between the 43 main species vertical distribution table 140 Table H. 44 intertidal zone between tables 141 Table H. 45 micro-fouling organisms recording table 142 Table H. 46 Ship fouling record table 143 Table H. 47 buoy fouling record table 144 Table H. 48 docks, piling fouling record table 145 Table H. 49 Analysis of fouling organisms recording table 146 Table H. 50 fouling species record table 147 Table H. 51 nekton trawls card 148 Table H. 52 fish bioassays record table 149 Table H. 53 shrimp bioassays record table 150 Table H. 54 crabs biological assays record table 151 Table H. 55 foot class biological assays record table 152 Table H. 56 The number of animals swim tables 153 Table H. Determination of 57 fish from long tables 154 Table H. 58 fish weight measuring tables 155 Table H. 59 fish fecundity record table 156 Table H. 60 157 acoustic surveys observation record sheet

Foreword

GB/T 12763 "oceanographic survey" is divided into 11 parts. --- Part 1. General; --- Part 2. oceanographic observations; --- Part 3. Marine meteorological observations; --- Part 4. Marine Chemistry Elements investigation; --- Part 5. Ocean Sound, light elements investigation; --- Part 6. Marine Biological Survey; --- Part 7. Marine survey data exchange; --- Part 8. Marine Geological Geophysical Survey; --- Part 9. Marine Ecological Survey Guidelines; --- Part 10. seabed topography survey; --- Part 11. Marine Engineering Geological Survey. Wherein Section 9, Section 10 and Section 11 corresponds to GB/T 12763-1991 is a new section. This section GB/T 612 763 Part of, and replaces GB/T 12763.6-1991 "specification for oceanographic survey marine life survey". This section compared with the GB/T 12763.6-1991 The main changes are as follows. --- Adjust the general framework of this section, an increase of "survey among 12 intertidal zone" between normative appendix "intertidal biological survey tide Level measurement method and apparatus several map "(see Appendix E) and information appendix" acoustic surveys and fishery resources assessments "(see Appendix G); the 1991 edition of the normative appendix "Seawater chlorophyll a, b, c of (Spectrophotometric Method)" "5 Leaf Green moved revised Su, primary productivity and new production of Determination "(1991 edition of Appendix A; this edition 5.2.2); the original," Part IV Plankton investigation "modified broken down into" 7 pico, micro and small plankton surveys "," 8 large and medium-sized plankton SURVEY ON "and" 9 ICHTHYOPLANKTON investigation "three chapters, including" 7 pico, micro and small plankton surveys "for the new Research project; --- "2 Normative references", increasing the GB 17378.7 specification for marine monitoring Part 7. Ecological survey of offshore pollution And biological monitoring; --- "3 Terms and definitions", add and modify the related terms (1991 version 3.4 and 3.7; Excerpts of 3.2, 3.3, 3.4,3.5,3.8,3.9,3.10 and 3.11); at the same time each term are supplemented with the corresponding English name; --- "4 general provisions", the survey projects and major equipment additions are two new items and new test equipment (1991 version 5.1 and 6; edition 4.2.1 and 4.3); increased to 200m deep in this edition of "Table 1 mining water level" in the Mining water level (see 4.2.4.1); further provides a number of investigations, detailed investigation quarter time (1991 version 5.5.2; 4.2.5 edition); modify and extend the scope of the laboratory (1991 version 6.2.1, this version 4.3.2); According to this revised. provision "oceanographic survey - Part 1 General" GB/T 12763.1-2007, the voyage report And the person in charge prepare a revised technical report on the survey results from the original respectively by the chief scientist and project leader chaired (1991 version 9.2.1 and 9.2.2, this version of 4.6.1.4 and 4.6.1.5) and the like; --- In "5 of chlorophyll, primary productivity and new production determination", the increase of the "high-performance liquid chromatography (HPLC) method," "chlorophyll a And the size of the primary productivity of classifying determination "and" new marine productivity (15N tracer method) "(see 5.2.3,5.4 and 5.5); --- In the "6 microbial survey", adding "SYBRGreenI direct counting method", "DAPI direct counting method", "bacterial volume Determination "," 3H- leucine tracer method "Determination of bacterial production of" ecological respiration rate determination "," bacterial specific growth rate, doubling time and Calculation of generation time "and" classification and identification of microorganisms "(see 6.3.4.1.1.1,6.3.4.1.1.3,6.3.4.1.1.4, 6.3.4.2.1.2,6.3.4.2.2.2,6.3.4.3 and 6.3.5); --- A new chapter "7 pico, micro and small plankton investigation"; --- In the "8 large and medium-sized plankton investigation", increasing the gear type, modified continuous observation frequency, sample handling, sample ID And mapping the value standard (1991 version 20.1.2,19.6,20.3.4,21.1 and 22.6; Excerpts 8.2.1.1,8.1.1. 3, 8.2.3.2,8.3.1 and 8.4.4); --- In the "9 ICHTHYOPLANKTON investigation", modify the collection nets abundance of formulas (1991 version 20.1.2 and 22.4; Excerpts of 9.2.1.1 and 9.4.1); --- "10 large benthic survey", adding "deep drag of the optical system and deep-sea benthic trawl" and "macrofauna Undersea photography, video and diving sampling "(see 10.2.1.2.5 and Appendix C.4); --- In the "11 small benthic survey", adding "diving sampling", "intertidal collected core samples," "sol Ludox-TM from Heart floating method "Diversity and multivariate statistical analysis and biological community structure (see 11.2.1.6 and 11.2.2.2,11.2.2.1, 11.3.4 and Appendix D. 4); modified sampling equipment (1991 version 28.1; edition 11.2.1); --- "Between 12 intertidal Biological Survey" for the new chapter; --- In the "13 fouling organisms investigation", modify the contents of the technical requirements (1991 version 31; Excerpts 13.1.1), an increase of tune Charles elements and cement test panels for fouling survey Coast Port Engineering Construction (13.1.2 and 13.2.1.1.1c)); --- In the "14 animals swim investigation", the "GSI" and "full feeding factor" into the "data compilation" (1991 edition The 37.3.1.5 and 37.3.1.6; Excerpts 14.4.2.4 and 14.4.2.5). Revised and added some important catches Gonad maturity and fish fat content of the criteria for the classification (see Appendix Excerpts F.1.2, Appendix F.1.3, Appendix F.1.4, Appendix F. 1.6, Appendix F. 1.7, Appendix F. 1.8 and Appendix F. 3); modified necton trawl type (1991 edition Appendix F7; Excerpts of Appendix F. 4); --- Modify and increase the informative appendix "Census of Marine Life, tabular analysis records" (1991 edition Appendix G; Excerpts Appendix H). --- This section should be GB/T 12763.1 and GB/T 12763.7 supporting the use. This section of the Appendix A, Appendix B, Appendix C, Appendix D, Annex E and Annex F are normative appendices, Appendix G and Appendix H of data Appendix. This part by the State Oceanic Administration. This part is administered by the National Oceanic Standards and Metrology Center. This part by the Third Institute of Oceanography, State Oceanic Administration responsible for revising, First Institute of Oceanography, State Oceanic Administration, State Oceanic Administration, Second Marine Institute, Ocean University of China, Chinese Academy of Fishery Sciences East China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences Yellow Sea Fisheries Research Institute Participate amendment. Drafting and revision of the main people in this section. Zhang Yusheng, Yang Qingliang, Chen Ruixiang, Zhu Mingyuan, Ip Tak Chan, Ningxiu Ren, Lin Jinghong, OCEANOGRAPHY Jiang Jinxiang, Zhi-Nan, Li Rongguan, Huangzong Guo, Zhengcheng Xing Zheng Yuan A, Zhaoxian Yong, Lv Ruihua, Lu bucket set, Shijun Xian Cai Yu Ming, Lin and Zhou. This part replaces the standard release case. --- GB/T 12763.6-1991. Oceanographic survey Part 6. Marine Biological Survey

1 Scope

GB/T 12763 provisions of this part of the general provisions of the Census of Marine Life, technical requirements and survey (measurement) elements, sampling, sample Basic requirements and methods of analysis and data compilation. This section applies to the marine environment, the basic elements of the Census of Marine Life Survey.

2 Normative references

The following documents contain provisions which, through GB/T 12763 reference in this text, constitute provisions of this part. For dated reference documents Member, all subsequent amendments (not including errata content) or revisions do not apply to this section, however, encouraged to reach under this section Parties to research agreement to use the latest versions of these documents. For undated reference documents, the latest versions apply to this section. GB/T 14014 silk, synthetic fiber mesh GB/T 12763.1 specification for oceanographic survey - Part 1. General GB/T 12763.7 specification for oceanographic survey - Part 7. Marine survey data processing GB 17378.7 specification for marine monitoring Part 7. Ecological survey of offshore pollution and biological monitoring

3 Terms and Definitions

GB/T 15919 and established the following terms and definitions apply GB/T 12763 in this section. 3.1 Autotrophic plant cells, an important class of pigments, which absorb and transfer light energy major substance plant photosynthesis. Chlorophyll a (Chla) is one of the main pigment. 3.2 Autotrophic organisms produce organic matter through photosynthesis ability. Usually in unit time (days or years) per unit area (or volume) in the production Health organics (generally represented organic carbon) mass, equivalent to the time within the same area (or volume) of the primary production. [GB/T 15919-1995, the definition 2.210] 3.3 It means the photosynthetic efficiency of plants photosynthetic pigments. In CO2 and adequate illumination conditions, and the same unit mass of chlorophyll per hour The ratio of the amount of carbon [conventional "carbon (mg)/chlorophyll (mg)/hr" indicates]. [GB/T 15919-1995, the definition 2.217] 3.4 In the euphotic recirculating nitrogen regeneration nitrogen, nitrogen provided by outside euphotic for the new nitrogen. First by the support that part of the regeneration of the nitrogen source Productivity known as reclaimed productivity level, supported by the nascent nitrogen that part called the new production of primary productivity. ......

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