GB 21047-2007 English PDF

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GB 21047-2007: [GB/T 21047-2007] Red drum
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Basic data

Standard ID: GB 21047-2007 (GB21047-2007)
Description (Translated English): [GB/T 21047-2007] Red drum
Sector / Industry: National Standard
Classification of Chinese Standard: B51
Classification of International Standard: 65.150
Word Count Estimation: 8,865
Date of Issue: 2007-06-26
Date of Implementation: 2007-10-01
Quoted Standard: GB/T 18654.3; GB/T 18654.12
Regulation (derived from): Chinese National Standards Bulletin 2007 No. 7 (No. 107 overall)
Issuing agency(ies): General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China, Standardization Administration of the People's Republic of China
Summary: This Chinese standard relates to eyespots intends kingfish morphological structural characteristics, growth and reproduction, cytologic features, biochemical, and genetic characteristics of detection methods. Eyespot intended for croakers germplasm identification and detection.

GB 21047-2007: [GB/T 21047-2007] Red drum

---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Red drum ICS 65.150 B51 National Standards of People's Republic of China Fish-eye Sciaenops Posted 2007-06-26 2007-10-01 implementation Administration of Quality Supervision, Inspection and Quarantine of People's Republic of China Standardization Administration of China released

Foreword

The first chapter 3,5,6 this standard are mandatory, the rest are recommended. The standard proposed by the People's Republic of China Ministry of Agriculture. This standard by the National Standardization Technical Committee on Fisheries mariculture sub-technical committee. This standard was drafted. Chinese Academy of Fishery Sciences Yellow Sea Fisheries Research Institute, the National First Institute of Oceanography, China Ocean the University. The main drafters of this standard. Liu Shilu, Wang Bo, high Tianxiang, Wang Jiankun. Fish-eye Sciaenops

1 Scope

Genetic characteristics and detection methods. This standard applies to eyespot Sciaenops fish germplasm identification and detection.

2 Normative references

The following documents contain provisions which, through reference in this standard and become the standard terms. For dated references, subsequent Amendments (not including errata content) or revisions do not apply to this standard, however, encourage the parties to the agreement are based on research Whether the latest versions of these documents. For undated reference documents, the latest versions apply to this standard. GB/T 18654.3 Inspection of germplasm for cultured fishes - Part 3. Determination of Characters GB/T 18654.12 Inspection of germplasm for cultured fishes - Part 12. karyotyping

3 Names and classification

3.1 Name Commonly known as. American red drum fish, red drum, red drum fish, kingfish and other spots. 3.2 Classification Location

4 morphological structural features

4.1 external features 4.1.1 external morphology Eyespot Sciaenops fish takes the shape of spindle-shaped, flat side, back slightly long, with the highest at the beginning of the dorsal fin, the first medium. Mouth terminal, gape large, small teeth Sharper, closely arranged. Nostril two pair, one pair after slightly oval. Lateral eye on the trailing edge and gape blunt, medium size, distribution On both sides of the head. N-shaped tail, in its growth process, the tail shape changes, fish larvae circular tail, to truncate juvenile, adult fish crescent tail. Caudal peduncle base side line side with one or more spots greater than eye diameter. Eyespot Sciaenops fish external morphology shown in Figure 1. Figure 1 spot Sciaenops fish external morphology 4.1.2 Number of Characters Eyespot Sciaenops fish countable characters are shown in Table 1. Table 1 spot Sciaenops fish morphology countable characters Anal fin A caudal dorsal D C P pectoral pelvic V lateral line scales outside the formula of the first gill arch rakers IX ~ X I-21 ~ 23 I ~ 8 17 15 ~ 17 I ~ 5 46 ~ 516/8 11 4.1.3 may amount proportional traits For full-length 21.31cm ~ 75.5cm, weight of the individual, the ratio of the measured traits 85g ~ 3900g values shown in Table 2. Table 2 spot Sciaenops fish morphology may amount proportional trait values (mean ± standard deviation) Full-length/body length body length/full-length body height/body height length/head length head length/snout length head length/eye diameter body length/caudal peduncle length of caudal peduncle length/tail height 1.14 ± 0.0154.24 ± 0.2464.83 ± 0.2373.66 ± 0.1833.55 ± 0.2556.62 ± 0.7674.25 ± 0.214 2.88 ± 0.226 4.2 Internal features 4.2.1 vertebrae 21. 4.2.2 swim bladder Long pocket. Bladder wall is smooth without compartment, a compartment.

5 Age and Growth

5.1 Age Age identified more scaly and Otolith 5.2 Growth In natural waters eyespot Sciaenops biggest fish fork length 149.9cm, weight 42.7kg. 5 years ago it grew faster after the slow growth rate SLOW. Same marine environment, age 1 to 3 years old average growth was no significant difference in gender, and older fish male and female differences are significant. In natural waters eyespot Sciaenops body length and weight growth equation see equation (1), formula (2). Where. FL --- fork length, in millimeters (mm); W --- weight in grams (g). Different age groups of eyespots Sciaenops body length, body weight found in Table 3. Table 3 pool seductive spot Sciaenops body length, weight Found Age/TSE 0123 Found body length/mm 30.30 310.70 472.20 551.70 Measured weight/g 0.52 530.17 1581.57 2486.46 5.3 body length and weight relationship Eyespot Sciaenops fish body length and weight relationship between the different stages as follows. a) the seed stage W = 0.0007BL4.1913 b) immature fish stage Indoor concrete ponds cultivation. W = 0.0125BL3.0776 Sea cages farming. W = 0.0165BL3.1852 c) broodstock stage W = 6.1673 × 10-6FL3.0984 Note. BL for the standard body length in centimeters (cm).

6 Reproduction

6.1 age of sexual maturity Eyespot Sciaenops fish mature earliest age. ♂ of 1 year, ♀ of three years. Eyespot Sciaenops fish mature at the latest Age. ♂ of five years, ♀ of six years. 6.2 sexual maturity and body length Natural sea male eyespots Sciaenops fish up to 350mm sexual maturity, 50% of the sexually mature male body length of 511mm ~ 529mm. Most males mature at age 1 or 2 years old, 3 years old when the whole maturity. The female body for up to 550mm after sexual maturity, 50% Sexually mature female body length of 825mm ~ 900mm. Some mature female age 3, age 6 when all the sexual maturity. 6.3 sexual maturity limits Mature individuals average maximum fork length. male 810mm, female 810mm; maximum weight. male 5.40kg, female 6.10kg; most Small fork length. male 593mm, female 598mm; Minimum Weight. male 2.35kg, female 3.43kg. 6.4 spawner Gonadal batches mature, spawning several times. Using artificial light and temperature control in the annual cycle, for multiple mature, increase the number of eggs. Floating eggs Eggs, egg diameter in 0.86mm ~ 1.08mm. 6.5 fecundity Eyespot Sciaenops spawning is shown in Table 4. Table 4 spot Sciaenops fish fecundity Age/age 3456789 Fecundity/× 104 measure word for small round pieces 10 12.5 ~ 5 ~ 2030 ~ 5060 ~ 7580 ~ 100 110 ~ 150 140 ~ 180 6.6 spawning temperature range Broodstock spawning season temperature range of 23 ℃ ~ 28 ℃, less than 21 ℃ will stop spawning.

7 cytogenetic characteristics

Somatic chromosome number 7.1 Like 2 = 48. 7.2 karyotype Karyotype formula is like 2 = 48t, or 24 pairs of chromosomes are the ends of the centromere of chromosome arm number (NF) is 48. Sciaenops fish-eye nuclear Type shown in Figure 2. Figure 2 spot Sciaenops fish karyotype

8 Characteristics of biochemical genetics

8.1 isozyme spectrum type Muscle isozymes of alcohol dehydrogenase isoenzyme (ADH), 6- phosphate isomerase isozymes (GPI), lactate dehydrogenase isoforms Enzyme (LDH), malic enzyme isozymes (ME) electrophoretic pattern are shown in Figure 3, Figure 4, Figure 5, Figure 6. Figure 3 alcohol dehydrogenase (ADH) 4 6-phosphate isomerase (GPI) Figure 5 lactate dehydrogenase (LDH) Figure 6 malic enzyme (ME) 8.2 Population genetic variability Eyespot Sciaenops 14 kinds of fish, a total of 24 isozyme loci. The proportion of polymorphic loci was 29.2%; the actual heterozygosity Ho = 0.057, average expected heterozygosity He = 0.108.

9 Detection

9.1 Determination of morphological traits According to GB/T 18654.3 execution. 9.2 karyotyping According to GB/T 18654.12 execution. 9.3 Determination of isozyme analysis The fish-eye Sciaenops vivo samples shipped back to the lab, whichever muscle tissue immediately frozen into 5mL tube, placed in -20 ℃ refrigerator warranty Deposit; removed from the test tube in the freezer, placed on ice to thaw a little box, and then double filter paper dipped 1.5mm × 8mm of tissue fluid Spotting. Sigma uses starch produced by hydrolysis of potato starch, gelatin concentration of 12%. Buffer system, using TC-7.0 And CAPM-6.0, electrophoresis 4.5h ~ 5.0h, electrophoresis and specific staining method and under constant current (30mA) constant temperature (4 ℃) conditions Enzyme nomenclature reference to Japan's Fisheries Resources Defense Council and Shaklee methods. Isozyme analysis using horizontal starch gel electrophoresis technique, not less than 30 eyespot Sciaenops fish biochemical genetic analysis. Isozyme staining solution formulations are shown in Table 5. Table 5 isozyme staining solution formulation English enzyme types, abbreviations and E. C. Drug concentration dose Codes Alcohol dehydrogenase alcoholdehydrogenase ADH E. C. 1.1.1.1 Ethyl alcohol (ethanol) 99% 1mL ~ 2mL Tris-HCl 0.2mol/L, pH8.5 25mL NAD (oxidized coenzyme I) basic preservation Solution - 25mL PMS (N- methyl phenazine methosulfate) Basic preservation solution --3 Drops to 5 drops Glucose-6-phosphate Isomerase glucose-6-phosphateisomerase GPI E. C. 5.3.1.9 Fructose 6-phosphate (fructose-6-phos- phate) - 125mg Tris-HCl 0.2mol/L, pH8.0 25mL MgCl2 1mol/L 0.25mL NADP (oxidized coenzyme Ⅱ) Basic insurance Deposit solution - 25mL ATPa (adenosine triphosphate) - 12.5mg G6PDb (glucose-6-phosphate dehydrogenase) - 4.65mg PMS basic preservation solution --3 drops to 5 drops Lactate dehydrogenase lactatedehydrogenase LDH E. C. 1.1.1.27 Lactic acid (lacticacid) - 0.625mL Tris-HCl 0.2mol/L, pH8.0 25mL NAD basic preservation solution - 25mL PMS basic preservation solution --3 drops to 5 drops Malic enzyme malicenzyme ME E. C. 1.1.1.40 Malic acid (malicacid) - 250mg Tris-HCl 0.2mol/L, pH8.5 25mL MgCl2 1mol/L 0.5mL NADP basic preservation solution - 25mL PMS basic preservation solution --3 drops to 5 drops aadenosinetriphosphate. bglucose-6-phosphatedehydrogenase. 10 decision rule The test results do not meet Chapter 3, Chapter 5, Chapter 6, any one of the requirements of the chapter, it is determined to be defective items; there are samples failed entry to Substandard samples. ......

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