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YC/T 159-2019 PDF English

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YC/T 159-2019: Tobacco and tobacco products - Determination of water soluble sugars - Continuous flow method
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YC/T 159: Historical versions

Standard IDUSDBUY PDFDeliveryStandard Title (Description)Status
YC/T 159-2019160 Add to Cart Auto, 9 seconds. Tobacco and tobacco products - Determination of water soluble sugars - Continuous flow method Valid
YC/T 159-200280 Add to Cart Auto, 9 seconds. Tobacco and tobacco products. Determination of water soluble sugars. Continuous flow method Obsolete

Similar standards

GB 5606.2   GB/T 5606.1   YC/T 605   

YC/T 159-2019: Tobacco and tobacco products - Determination of water soluble sugars - Continuous flow method


---This is an excerpt. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.), auto-downloaded/delivered in 9 seconds, can be purchased online: https://www.ChineseStandard.net/PDF.aspx/YCT159-2019
TOBACCO INDUSTRY STANDARD OF THE PEOPLE’S REPUBLIC OF CHINA ICS 65.160 X 85 Replacing YC/T 159-2002 Tobacco and tobacco products - Determination of water soluble sugars - Continuous flow method Issued on. DECEMBER 26, 2019 Implemented on. JANUARY 15, 2020 Issued by. State Tobacco Monopoly Administration

Table of Contents

Foreword... 3 1 Scope... 5 2 Normative references... 5 3 Principle... 5 4 Reagents... 5 5 Instruments and materials... 7 7 Result calculation and expression... 8 8 Precision and recovery rate... 9 9 Test report... 10 Annex A (informative) Flow chart of continuous flow analysis of water-soluble sugar... 12

Foreword

This Standard was drafted in accordance with the rules given in GB/T 1.1-2009 and GB/T 20001.4-2015. This Standard replaces YC/T 159-2002 “Tobacco and tobacco products - Determination of water soluble sugars - Continuous flow method”. Compared with YC/T 159-2002, in addition to editorial modifications, the main technical changes are as follows. - modified green/green pipe sodium hydroxide solution concentration (see 4.2 of this Edition, 4.2 of Edition 2002); - modified reagent for measuring red/red pipe diameter when reducing sugar (see 4.6 of this Edition, 4.6 of Edition 2002); - modified 85°C heating tank volume (see 5.1 of this Edition, 5/1 of Edition 2002); - added heat-dissipation device (see 5.1 of this Edition, 5.1 of Edition 2002). Please note that some content of this document may involve patents. The issuer of this document does not assume responsibility for identifying these patents. This Standard was proposed by State Tobacco Monopoly Administration. This Standard shall be under the jurisdiction of Subcommittee on Cigarette of National Technical Committee on Tobacco of Standardization Administration of China (SAC/TC 144/SC 1). The drafting organizations of this Standard. National Tobacco Quality Supervision and Inspection Center, Shanghai Tobacco Group Beijing Cigarette Factory, Yunnan China Tobacco Industry Co., Ltd., Yunnan China Tobacco Recycled Tobacco Co., Ltd., Zhengzhou Tobacco Research Institute, Yunnan Tobacco Quality Supervision and Inspection Station, Guizhou China Tobacco Industry Co., Ltd., Henan China Tobacco Industry Co., Ltd., Henan Tobacco Quality Supervision and Inspection Station, Northwest Tobacco Quality Supervision and Inspection Station, Guangdong Tobacco Quality Supervision and Inspection Station, Shandong China Tobacco Industry Co., Ltd. Main drafters of this Standard. He Shengbao, Zhang Wei, Luo Anna, Liu Nan, Feng Xiaomin, Wang Yingyuan, Ma Yanjun, Qin Yunhua, Liu Enfen, Wang Ran, Guo Junwei, Peng Qianrong, Li Wenwei, Peng Lijuan, Ma Li, Du Guorong, Wang Fei, Wang Kai, Peng Yuntie, Shi Hongyan, Liu Wei, Wang Hongbo, Zhang Jianfeng, Mao Jian, Ji Houwei, Wu Shouming, Guo Shuwen, Zhao Haijuan. Version of standard substituted by this Standard is. - YC/T 159-2002. Tobacco and tobacco products - Determination of water soluble sugars - Continuous flow method

1 Scope

This Standard specifies the method to determine water-soluble sugar (including water-soluble total sugar and water-soluble reducing sugar) in tobacco and tobacco products. This Standard is applicable to the determination of water-soluble sugar in tobacco and tobacco products. The detection limit of this method is 0.0063%. quantitation limit is 0.0210%.

2 Normative references

The following referenced documents are indispensable for the application of this document. For dated references, only the edition cited applies. For undated references, the latest edition of the referenced document (including any amendments) applies. GB/T 6682, Water for analytical laboratory use - Specification and test methods YC/T 31, Tobacco and tobacco products - Preparation of test sample and determination of water content - Oven method

3 Principle

Use 5% aqueous acetic acid to extract the sample. The sugar in the extract reacts with p-hydroxybenzoic acid hydrazide. Produce yellow azo compounds in alkaline media at 85°C.

4 Reagents

Except for special requirements, analytically pure reagents shall be used. The water shall comply with the regulations for grade one water in GB/T 6682. 4.1 Polyoxyethylene lauryl ether solution (Brij35 solution) Add 250g of polyoxyethylene lauryl ether to 1L of water. Heat and stir until it is dissolved. 4.4 Extract (5% acetic acid solution) Take 50mL of glacial acetic acid. Use water to dilute to 1L, for sample extraction or preparation of working standard solution. 4.5 Flushing solution (activated 5% acetic acid solution) Take 1L of 5% acetic acid solution (4.4). Add 0.5mL of Brij35 solution (4.1), for flushing of continuous flow analyzer pipeline. 4.6 0.2mol/L hydrochloric acid solution 4.7 0.5mol/L hydrochloric acid solution In the fume hood, slowly add 42mL of fuming hydrochloric acid (mass fraction is 37%) to 500mL of water. Use water to dilute to 1L. 4.10 5% p-hydroxybenzoic acid hydrazide solution Add 250mL of 0.5mol/L hydrochloric acid solution (4.7) to a beaker. Add 25g of paraben hydrazide (4.9) to make it dissolved. Add 10.5g of citric acid (C6H8O7 • H2O). After dissolution, transfer to a 500mL volumetric flask. Use 0.5mol/L hydrochloric acid solution to dilute to the scale. Store at 0°C~4°C. 4.11 D-glucose, purity ≥99.5%. 4.12 Standard stock solution. Weigh 20.0g of dry D-glucose (4.11) in a beaker, to the nearest of 0.0001g. Use water to dissolve then transfer into a 500mL volumetric flask and set volume to the scale. The standard stock solution is stored in the refrigerator at 0°C~4°C. The validity is 1 month. 4.13 Series standard working solution. Use 5% acetic acid solution (4.4) to prepare at least 5 standard working solution from stock solution. The concentration range shall cover the expected sample content. The standard stock solution is stored in the refrigerator at 0°C~4°C. The validity is 2 weeks.

5 Instruments and materials

5.1 Continuous flow analyzer, consisting of the following parts. 5.2 Balance, with a sensitivity of 0.0001g. 5.3 Oscillator. 5.4 Quantitative filling injector or pipette. 5.5 50mL Erlenmeyer flask with stopper. 5.6 Rapid qualitative filter paper. 6 Analysis steps 6.1 Sample preparation Prepare sample according to YC/T 31 and measure its moisture content. 6.2 Sample processing Weigh 0.25g of sample in the 50mL Erlenmeyer flask with stopper (5.5), to the nearest of 0.0001g. Add 25mL of 5% acetic acid solution (4.4). 6.3 Instrument analysis Operate the series of standard working solution (4.13) and subsequent filtrate of sample processing (6.2) on the instrument. See Annex A for the analysis flowchart. If the sample concentration exceeds the standard working solution concentration range, it shall be diluted before measurement.

7 Result calculation and expression

7.1 Calculation of water-soluble sugar The water-soluble sugar content is calculated according to formula (1). 7.2 Result expression Take the average of two parallel determination results as the measurement result. If the measured water-soluble sugar content is greater than or equal to 10.0%, the result is to the nearest of 0.1%. If it is less than 10.0%, the result is to the nearest of 0.01%.

8 Precision and recovery rate

See Table 1 for the precision results of this method.

9 Test report

The test report shall indicate.

Annex A

(informative) Flow chart of continuous flow analysis of water-soluble sugar Figure A.1 shows the flow chart of continuous flow analysis of water-soluble sugars injected at high flow rate. Figure A.2 shows the flow chart of continuous flow method analysis of water-soluble sugar injection at low flow rate. YC/T 159-2019 TOBACCO INDUSTRY STANDARD OF THE PEOPLE’S REPUBLIC OF CHINA ICS 65.160 X 85 Replacing YC/T 159-2002 Tobacco and tobacco products - Determination of water soluble sugars - Continuous flow method Issued on. DECEMBER 26, 2019 Implemented on. JANUARY 15, 2020 Issued by. State Tobacco Monopoly Administration

Table of Contents

Foreword... 3 1 Scope... 5 2 Normative references... 5 3 Principle... 5 4 Reagents... 5 5 Instruments and materials... 7 7 Result calculation and expression... 8 8 Precision and recovery rate... 9 9 Test report... 10 Annex A (informative) Flow chart of continuous flow analysis of water-soluble sugar... 12

Foreword

This Standard was drafted in accordance with the rules given in GB/T 1.1-2009 and GB/T 20001.4-2015. This Standard replaces YC/T 159-2002 “Tobacco and tobacco products - Determination of water soluble sugars - Continuous flow method”. Compared with YC/T 159-2002, in addition to editorial modifications, the main technical changes are as follows. - modified green/green pipe sodium hydroxide solution concentration (see 4.2 of this Edition, 4.2 of Edition 2002); - modified reagent for measuring red/red pipe diameter when reducing sugar (see 4.6 of this Edition, 4.6 of Edition 2002); - modified 85°C heating tank volume (see 5.1 of this Edition, 5/1 of Edition 2002); - added heat-dissipation device (see 5.1 of this Edition, 5.1 of Edition 2002). Please note that some content of this document may involve patents. The issuer of this document does not assume responsibility for identifying these patents. This Standard was proposed by State Tobacco Monopoly Administration. This Standard shall be under the jurisdiction of Subcommittee on Cigarette of National Technical Committee on Tobacco of Standardization Administration of China (SAC/TC 144/SC 1). The drafting organizations of this Standard. National Tobacco Quality Supervision and Inspection Center, Shanghai Tobacco Group Beijing Cigarette Factory, Yunnan China Tobacco Industry Co., Ltd., Yunnan China Tobacco Recycled Tobacco Co., Ltd., Zhengzhou Tobacco Research Institute, Yunnan Tobacco Quality Supervision and Inspection Station, Guizhou China Tobacco Industry Co., Ltd., Henan China Tobacco Industry Co., Ltd., Henan Tobacco Quality Supervision and Inspection Station, Northwest Tobacco Quality Supervision and Inspection Station, Guangdong Tobacco Quality Supervision and Inspection Station, Shandong China Tobacco Industry Co., Ltd. Main drafters of this Standard. He Shengbao, Zhang Wei, Luo Anna, Liu Nan, Feng Xiaomin, Wang Yingyuan, Ma Yanjun, Qin Yunhua, Liu Enfen, Wang Ran, Guo Junwei, Peng Qianrong, Li Wenwei, Peng Lijuan, Ma Li, Du Guorong, Wang Fei, Wang Kai, Peng Yuntie, Shi Hongyan, Liu Wei, Wang Hongbo, Zhang Jianfeng, Mao Jian, Ji Houwei, Wu Shouming, Guo Shuwen, Zhao Haijuan. Version of standard substituted by this Standard is. - YC/T 159-2002. Tobacco and tobacco products - Determination of water soluble sugars - Continuous flow method

1 Scope

This Standard specifies the method to determine water-soluble sugar (including water-soluble total sugar and water-soluble reducing sugar) in tobacco and tobacco products. This Standard is applicable to the determination of water-soluble sugar in tobacco and tobacco products. The detection limit of this method is 0.0063%. quantitation limit is 0.0210%.

2 Normative references

The following referenced documents are indispensable for the application of this document. For dated references, only the edition cited applies. For undated references, the latest edition of the referenced document (including any amendments) applies. GB/T 6682, Water for analytical laboratory use - Specification and test methods YC/T 31, Tobacco and tobacco products - Preparation of test sample and determination of water content - Oven method

3 Principle

Use 5% aqueous acetic acid to extract the sample. The sugar in the extract reacts with p-hydroxybenzoic acid hydrazide. Produce yellow azo compounds in alkaline media at 85°C.

4 Reagents

Except for special requirements, analytically pure reagents shall be used. The water shall comply with the regulations for grade one water in GB/T 6682. 4.1 Polyoxyethylene lauryl ether solution (Brij35 solution) Add 250g of polyoxyethylene lauryl ether to 1L of water. Heat and stir until it is dissolved. 4.4 Extract (5% acetic acid solution) Take 50mL of glacial acetic acid. Use water to dilute to 1L, for sample extraction or preparation of working standard solution. 4.5 Flushing solution (activated 5% acetic acid solution) Take 1L of 5% acetic acid solution (4.4). Add 0.5mL of Brij35 solution (4.1), for flushing of continuous flow analyzer pipeline. 4.6 0.2mol/L hydrochloric acid solution 4.7 0.5mol/L hydrochloric acid solution In the fume hood, slowly add 42mL of fuming hydrochloric acid (mass fraction is 37%) to 500mL of water. Use water to dilute to 1L. 4.10 5% p-hydroxybenzoic acid hydrazide solution Add 250mL of 0.5mol/L hydrochloric acid solution (4.7) to a beaker. Add 25g of paraben hydrazide (4.9) to make it dissolved. Add 10.5g of citric acid (C6H8O7 • H2O). After dissolution, transfer to a 500mL volumetric flask. Use 0.5mol/L hydrochloric acid solution to dilute to the scale. Store at 0°C~4°C. 4.11 D-glucose, purity ≥99.5%. 4.12 Standard stock solution. Weigh 20.0g of dry D-glucose (4.11) in a beaker, to the nearest of 0.0001g. Use water to dissolve then transfer into a 500mL volumetric flask and set volume to the scale. The standard stock solution is stored in the refrigerator at 0°C~4°C. The validity is 1 month. 4.13 Series standard working solution. Use 5% acetic acid solution (4.4) to prepare at least 5 standard working solution from stock solution. The concentration range shall cover the expected sample content. The standard stock solution is stored in the refrigerator at 0°C~4°C. The validity is 2 weeks.

5 Instruments and materials

5.1 Continuous flow analyzer, consisting of the following parts. 5.2 Balance, with a sensitivity of 0.0001g. 5.3 Oscillator. 5.4 Quantitative filling injector or pipette. 5.5 50mL Erlenmeyer flask with stopper. 5.6 Rapid qualitative filter paper. 6 Analysis steps 6.1 Sample preparation Prepare sample according to YC/T 31 and measure its moisture content. 6.2 Sample processing Weigh 0.25g of sample in the 50mL Erlenmeyer flask with stopper (5.5), to the nearest of 0.0001g. Add 25mL of 5% acetic acid solution (4.4). 6.3 Instrument analysis Operate the series of standard working solution (4.13) and subsequent filtrate of sample processing (6.2) on the instrument. See Annex A for the analysis flowchart. If the sample concentration exceeds the standard working solution concentration range, it shall be diluted before measurement.

7 Result calculation and expression

7.1 Calculation of water-soluble sugar The water-soluble sugar content is calculated according to formula (1). 7.2 Result expression Take the average of two parallel determination results as the measurement result. If the measured water-soluble sugar content is greater than or equal to 10.0%, the result is to the nearest of 0.1%. If it is less than 10.0%, the result is to the nearest of 0.01%.

8 Precision and recovery rate

See Table 1 for the precision results of this method.

9 Test report

The test report shall indicate.

Annex A

(informative) Flow chart of continuous flow analysis of water-soluble sugar Figure A.1 shows the flow chart of continuous flow analysis of water-soluble sugars injected at high flow rate. Figure A.2 shows the flow chart of continuous flow method analysis of water-soluble sugar injection at low flow rate. ......
Source: Above contents are excerpted from the full-copy PDF -- translated/reviewed by: www.ChineseStandard.net / Wayne Zheng et al.
Image 1     Image 2     Image 3     

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