Home Cart Quotation About-Us
www.ChineseStandard.net
SEARCH

SN/T 5706-2023 PDF English

US$140.00 · In stock · Download in 9 seconds
SN/T 5706-2023: Cosmetics microbiology - Detection of Escherichia coli
Delivery: 9 seconds. True-PDF full-copy in English & invoice will be downloaded + auto-delivered via email. See step-by-step procedure
Status: Valid
Standard IDUSDBUY PDFDeliveryStandard Title (Description)Status
SN/T 5706-2023140 Add to Cart Auto, 9 seconds. Cosmetics microbiology - Detection of Escherichia coli Valid

Similar standards

QB/T 5291   GB 5296.3   SN/T 1780   

SN/T 5706-2023: Cosmetics microbiology - Detection of Escherichia coli

---This is an excerpt. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.), auto-downloaded/delivered in 9 seconds, can be purchased online: https://www.ChineseStandard.net/PDF.aspx/SNT5706-2023
SN ENTRY-EXIT INSPECTION AND QUARANTINE INDUSTRY STANDARD OF THE PEOPLE’S REPUBLIC OF CHINA ICS 71.100.70 CCS Y 42 Cosmetics microbiology - Detection of escherichia coli ISSUED ON: DECEMBER 29, 2023 IMPLEMENTED ON: JULY 01, 2024 Issued by: General Administration of Customs of PRC

Table of Contents

Foreword ... 3 1 Scope ... 4 2 Normative references ... 4 3 Terms and definitions ... 4 4 Method summary ... 4 5 Equipment and materials ... 4 6 Culture medium and reagents ... 5 7 Inspection procedure ... 5 8 Result report ... 6 Appendix A (Normative) Main culture medium and reagents ... 7 Cosmetics microbiology - Detection of Escherichia coli

1 Scope

This document describes the qualitative test method for Escherichia coli in cosmetics. This document is applicable to the test of Escherichia coli in cosmetics.

2 Normative references

The contents of the following documents constitute essential clauses of this document through normative references in the text. Among them, for dated references, only the version corresponding to the date applies to this document; for undated references, the latest version (including all amendments) applies to this document. SN/T 5075 Specification for sample preparation for microbiological inspection of cosmetics

3 Terms and definitions

There are no terms and definitions that need to be defined in this document.

4 Method summary

This method uses the activity of β-glucuronidase in Escherichia coli, which can decompose 5-bromo-4-chloro-3-indole-β-D-glucuronide and form blue-green colonies on trypsin bile salt X-glucuronide agar plates, for qualitative testing. Note: This method is not applicable to a small number of β-glucuronidase-negative Escherichia coli, such as O157.

5 Equipment and materials

In addition to the conventional sterilization and culture equipment of the microbiology laboratory, other equipment and materials are as follows. 5.1 Constant temperature incubator: 36 °C ± 1 °C. 5.2 Constant temperature device: 48 °C ± 2 °C. 5.3 Refrigerator: 2 °C ~ 8 °C. 5.4 Homogenizer or emulsion disperser (speed above 1000 r/min). 5.5 Electronic balance: Sensitivity 0.1 g. 5.6 Sterile conical flask: 250 mL or 300 mL. 5.7 Sterile pipette: 10 mL (with 0.1 mL scale). 5.8 Sterile culture dish: Diameter 90 mm. 5.9 Sterile homogenizing bag or homogenizing cup. 5.10 Sterile inoculation loop: 10 μL (about 3 mm in diameter). 5.11 pH meter or precision pH test paper. 5.12 Quality control strains: Escherichia coli ATCC25922 or CGMCC 1.2385 or equivalent strains as positive control; Citrobacter freundii ATCC 43864 or CICC 10296 or equivalent strains as negative control.

6 Culture medium and reagents

6.1 Soybean casein lecithin Tween 80 (SCDLP) liquid culture medium: According to A.1. 6.2 Tryptone bile X-glucuronide (TBX) agar: According to A.2.

7 Inspection procedure

7.1 Inspection procedure for Escherichia coli in cosmetics The inspection procedure for Escherichia coli in cosmetics is as shown in Figure 1.

Appendix A

(Normative) Main culture medium and reagents A.1 Soybean casein lecithin Tween 80 (SCDLP) liquid culture medium A.1.1 Ingredients Casein peptone: 17.0 g Soybean protein peptone: 3.0 g Sodium chloride: 5.0 g Potassium hydrogen phosphate: 2.5 g Glucose: 2.5 g Lecithin: 1.0 g Tween 80: 7.0 g Distilled water: 1000 mL A.1.2 Preparation method First, heat and dissolve lecithin in a small amount of distilled water. Then mix with other ingredients. Heat and dissolve, 90 mL per bottle. Sterilize at 121 °C for 15 min. Note that the oscillation shall be performed to mix the Tween 80 precipitated at the bottom layer thoroughly. Cool it to about 25 °C to prepare for use. The pH of the sterilized culture medium at 25 °C is 7.2 ± 0.2. A.2 Trypsin bile salt X-Glucuronide (TBX) agar A.2.1 Ingredients Trypsin: 20.0 g Bile salt No.3: 1.5 g 5-Bromo-4-chloro-3-indole-D-glucuronide (BCIG): 75.0 mg Agar: 8.0 g ~ 18.0 g Distilled water: 1000 mL A.2.2 Preparation ......
Source: Above contents are excerpted from the full-copy PDF -- translated/reviewed by: www.ChineseStandard.net / Wayne Zheng et al.
Image 1     Image 2     Image 3     

Tips & Frequently Asked Questions:

Question 1: How long will the true-PDF of English version of SN/T 5706-2023 be delivered?Answer: The full copy PDF of English version of SN/T 5706-2023 can be downloaded in 9 seconds, and it will also be emailed to you in 9 seconds (double mechanisms to ensure the delivery reliably), with PDF-invoice.

Question 2: Can I share the purchased PDF of SN/T 5706-2023_English with my colleagues?Answer: Yes. The purchased PDF of SN/T 5706-2023_English will be deemed to be sold to your employer/organization who actually paid for it, including your colleagues and your employer's intranet.

Question 3: Does the price include tax/VAT?Answer: Yes. Our tax invoice, downloaded/delivered in 9 seconds, includes all tax/VAT and complies with 100+ countries' tax regulations (tax exempted in 100+ countries) -- See Avoidance of Double Taxation Agreements (DTAs): List of DTAs signed between Singapore and 100+ countries

Question 4: Do you accept my currency other than USD?Answer: Yes. www.ChineseStandard.us -- SN/T 5706-2023 -- Click this link and select your country/currency to pay, the exact amount in your currency will be printed on the invoice. Full PDF will also be downloaded/emailed in 9 seconds.

How to buy and download a true PDF of English version of SN/T 5706-2023?

A step-by-step guide to download PDF of SN/T 5706-2023_EnglishStep 1: Visit website https://www.ChineseStandard.net (Pay in USD), or https://www.ChineseStandard.us (Pay in any currencies such as Euro, KRW, JPY, AUD).
Step 2: Search keyword "SN/T 5706-2023".
Step 3: Click "Add to Cart". If multiple PDFs are required, repeat steps 2 and 3 to add up to 12 PDFs to cart.
Step 4: Select payment option (Via payment agents Stripe or PayPal).
Step 5: Customize Tax Invoice -- Fill up your email etc.
Step 6: Click "Checkout".
Step 7: Make payment by credit card, PayPal, Google Pay etc. After the payment is completed and in 9 seconds, you will receive 2 emails attached with the purchased PDFs and PDF-invoice, respectively.
Step 8: Optional -- Go to download PDF.
Step 9: Optional -- Click Open/Download PDF to download PDFs and invoice.
See screenshots for above steps: Steps 1~3    Steps 4~6    Step 7    Step 8    Step 9