GB/T 5413.2-1997 PDF EnglishUS$80.00 · In stock · Download in 9 seconds
GB/T 5413.2-1997: Milk powder and formula foods for infant and young children. Determination of whey protein Delivery: 9 seconds. True-PDF full-copy in English & invoice will be downloaded + auto-delivered via email. See step-by-step procedure
Similar standardsGB/T 5413.2-1997: Milk powder and formula foods for infant and young children. Determination of whey protein---This is an excerpt. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.), auto-downloaded/delivered in 9 seconds, can be purchased online: https://www.ChineseStandard.net/PDF.aspx/GBT5413.2-1997 GB NATIONAL STANDARD OF THE PEOPLE’S REPUBLIC OF CHINA Replacing GB 5413-85 Milk powder and formula foods for infant and young children - Determination of whey protein APPROVED ON: MAY 28, 1997 IMPLEMENTED ON: SEPTEMBER 01, 1998 Approved by: State Bureau of Technical Supervision Table of ContentsForeword ... 3 1 Scope ... 4 2 Method summary ... 4 3 Reagents ... 4 4 Instruments ... 5 5 Operation steps ... 5 6 Expression of analysis results ... 6ForewordThis Standard refers to the whey protein determination method of Morinaga, Japan. This series of standards will replace GB 5413-85 from the date of implementation. This Standard was proposed by China National Light Industry Council. This Standard shall be under the jurisdiction of National Technical Committee on Dairy Products of Standardization Administration of China. Main drafting organizations of this Standard: Harbin Morinaga Dairy Co., Ltd., National Dairy Products Quality Supervision and Inspection Center. The drafting organizations of this Standard: Food Hygiene Supervision and Inspection Institute of the Ministry of Health, Zhejiang Light Industry Research Institute, Nestlé (China) Investment Services Co., Ltd. Main drafters of this Standard: Yu Likun, Zhang Yanjie, Zhao Qin. Milk powder and formula foods for infant and young children - Determination of whey protein1 ScopeThis Standard specifies determination method for the ratio of casein to whey protein content. This Standard is applicable to the determination of the ratio of casein to whey protein content in milk powder and formula foods for infant and young children.2 Method summaryAfter using SDS-PAGE, Laemmli method to sample, use an optical densitometer to determine each band of casein and whey protein separated in order of molecular weight. Calculate the ratio of casein to whey protein content.3 ReagentsAll reagents, if the specification is not specified, refer to analytically-pure reagents. All experimental water, if other requirements are not specified, refer to grade three water. 3.1 Electrophoresis buffer: Dissolve 7.5g of Tris (hydroxymethyl) aminomethane, 36g of glycine and 2.5g of SDS in distilled water. Set volume to 500mL. Before use, use distilled water to dilute to 5 times. 3.2 SDS sample buffer: 0.125mol/L Trsi-hydrochloric acid (pH 6.8), glycerin with volume fraction of 20%, 4% (m/V) SDS, 2-mercaptoethanol with volume fraction of 10%, bromophnol blue with mass fraction of 0.0025%. 3.3 Stain: Coomassie brilliant blue R-250 with mass fraction of 0.1%, methanol with volume fraction of 10%, acetic acid with volume fraction of 7.5%. 3.4 Decolorant: methanol with volume fraction of 10%, acetic acid with volume fraction of 7.5%. 3.5 Casein. 3.6 Refined whey protein (WPC). ......Source: Above contents are excerpted from the full-copy PDF -- translated/reviewed by: www.ChineseStandard.net / Wayne Zheng et al. 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