Home Cart Quotation About-Us
www.ChineseStandard.net
SEARCH

GB 5413.18-2010 PDF English

US$85.00 · In stock · Download in 9 seconds
GB 5413.18-2010: National food safety standard -- Determination of vitamin C in foods for infants and young children, milk and milk products
Delivery: 9 seconds. True-PDF full-copy in English & invoice will be downloaded + auto-delivered via email. See step-by-step procedure
Status: Valid

GB 5413.18: Historical versions

Standard IDUSDBUY PDFDeliveryStandard Title (Description)Status
GB 5413.18-201085 Add to Cart Auto, 9 seconds. National food safety standard -- Determination of vitamin C in foods for infants and young children, milk and milk products Valid
GB/T 5413.18-1997199 Add to Cart 2 days Milk powder and formula foods for infant and young children--Determination of vitamin C content Obsolete

Similar standards

GB 5413.20   GB 5413.19   GB 5413.22   GB 5413.38   

GB 5413.18-2010: National food safety standard -- Determination of vitamin C in foods for infants and young children, milk and milk products


---This is an excerpt. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.), auto-downloaded/delivered in 9 seconds, can be purchased online: https://www.ChineseStandard.net/PDF.aspx/GB5413.18-2010
GB NATIONAL STANDARD OF THE PEOPLE’S REPUBLIC OF CHINA National food safety standard Determination of vitamin C in foods for infants and young children, milk and milk products Issued on. MARCH 26, 2010 Implemented on. JUNE 1, 2010 Issued by. Ministry of Health of PRC

Table of Contents

Foreword... 3 1 Scope... 4 2 Normative references... 4 3 Principle... 4 4 Reagents and materials... 4 5 Apparatus... 5 6 Analysis procedures... 6 7 Result representation... 7 8 Precision... 8 9 Other... 8

Foreword

This Standard replaces GB/T 5413.18-1997 "Milk powder and formula foods for infants and young children - Determination of vitamin C content". Compared with GB/T 5413.18-1997, the main changes of this Standard are as follows. - defined the activity unit of enzyme; - changed the concentration of o-phenylenediamine solution; - changed the treatment of specimens containing starch; - added -- the reaction time after adding boric acid-sodium acetate solution; - added -- the reaction time after adding o-phenylenediamine solution; This Standard replaces the following previous standards. - GB 5413-1985, GB/T 5413.18-1997. National food safety standard Determination of vitamin C in foods for infants and young children, milk and milk products

1 Scope

This Standard specifies the determination method of vitamin C in foods for infants and young children, milk and milk products. This standard is applicable to the determination of vitamin C in foods for infants and young children, milk and milk products. The determination result of this Standard indicates the total content of reduction-type vitamin C and oxidation- type vitamin C.

2 Normative references

The following referenced documents are indispensable for the application of this document. For dated references, only the edition cited applies. For undated references, the latest edition of the referenced document (including any amendments) applies.

3 Principle

Vitamin C (ascorbic acid) is oxidized to be dehydroascorbic acid in the presence of activated carbon. The dehydroascorbic acid reacts with o-phenylenediamine to generate fluorescent substances. Use fluorescent spectrophotometer to determine its fluorescence intensity.

4 Reagents and materials

Unless otherwise specified, the reagents used in the method are analytically pure; the water used is Grade 3 water regulated in GB/T 6682. 4.1 Amylase. enzyme activity is 1.5U/mg; adjust the dosage in accordance with the activity unit size. 4.2 Metaphosphoric acid-acetic acid solution A. weigh 15 g of metaphosphoric acid and 40 mL of acetic acid (36%) in 200 mL of water; dissolve and dilute to 500 mL for use. 4.3 Metaphosphoric acid-acetic acid solution B. weigh 15 g of metaphosphoric acid and 40 mL of acetic acid (36%) in 100 mL of water; dissolve and dilute to 250 mL for use. 4.5 Sodium acetate solution. use water to dissolve 500 g of sodium acetate trihydrate till it is diluted to 1 L. 4.6 Boric acid-sodium acetate. weigh 3.0 g of boric acid; use the sodium acetate solution (4.5) to dissolve till it is diluted to 100 mL; prepare when required. 4.8 Vitamin C standard solution (100 µg/mL). weigh 0.050 g of Vitamin C standard product; use metaphosphoric acid - acetic acid solution A (4.2) to dissolve and make constant volume to 50 mL;

5 Apparatus

5.1 Fluorescence spectrophotometer. 5.2 Scale. inductance of 0.1 mg. 5.4 Incubator. 45°C±1°C.

6 Analysis procedures

6.1 Specimen Treatment 6.1.1 Starch-containing specimen. WEIGH about 5 g (accurate to 0.0001 g) of well mixed or about 20 g (accurate to 0.0001 g) of liquid specimen (containing about 2 mg of Vitamin C) in a 150 mL erlenmeyer flask. 6.1.2 Starch-free specimen. WEIGH about 5 g of well mixed solid specimen (accurate to 0.0001 g). USE metaphosphoric acid-acetic acid A (4.2) to dissolve. MAKE constant volume to 100 mL. 6.2 Preparation of solution for test 6.2.1 TRANSFER the aforementioned specimens (6.1.1, 6.1.2) and Vitamin C standard solution (4.8) to a 250 mL erlenmeyer flask having about 2 g of acidic activated carbon (4.4). Severely VIBRATE it. FILTER it (discard about 5 mL of initial filtration) and it shall be the filtration of specimen and standard solution. Accurately PIPETTE 5.0 mL of the filtration of specimen and standard solution. Respectively PLACE in the 25 mL and 50 mL flasks having 5.0 mL of boric acid-sodium acetate solution (4.6). Place for 30 min. USE distilled water for constant volume. Use it as the blank solution of specimen and standard solution. 6.3 Determination 6.3.1 Drawing of standard curve Immediately transfer the standard series of test solution (6.2.4) into the quartz curette of the fluorescence spectrophotometer. Determine its fluorescence value when excitation wavelength is 350 nm, emission wavelength is 430 nm. Standard fluorescent value respectively minus standard blank fluorescence value as Y-axis; take the corresponding vitamin C mass concentration as X- coordinate to draw the standard curve.

7 Result representation

The vitamin C content in specimen shall be calculated according to equation (1).

8 Precision

The absolute difference between two independent determination results obtained under repeatability conditions shall not exceed 10% of the arithmetic mean.

9 Other

The detection limit of this Standard is 0.1 mg/100 g. ......
Source: Above contents are excerpted from the full-copy PDF -- translated/reviewed by: www.ChineseStandard.net / Wayne Zheng et al.
Image 1     Image 2     Image 3     

Tips & Frequently Asked Questions:

Question 1: How long will the true-PDF of English version of GB 5413.18-2010 be delivered?Answer: The full copy PDF of English version of GB 5413.18-2010 can be downloaded in 9 seconds, and it will also be emailed to you in 9 seconds (double mechanisms to ensure the delivery reliably), with PDF-invoice.

Question 2: Can I share the purchased PDF of GB 5413.18-2010_English with my colleagues?Answer: Yes. The purchased PDF of GB 5413.18-2010_English will be deemed to be sold to your employer/organization who actually paid for it, including your colleagues and your employer's intranet.

Question 3: Does the price include tax/VAT?Answer: Yes. Our tax invoice, downloaded/delivered in 9 seconds, includes all tax/VAT and complies with 100+ countries' tax regulations (tax exempted in 100+ countries) -- See Avoidance of Double Taxation Agreements (DTAs): List of DTAs signed between Singapore and 100+ countries

Question 4: Do you accept my currency other than USD?Answer: Yes. www.ChineseStandard.us -- GB 5413.18-2010 -- Click this link and select your country/currency to pay, the exact amount in your currency will be printed on the invoice. Full PDF will also be downloaded/emailed in 9 seconds.

Question 5: Should I purchase the latest version GB 5413.18-2010?Answer: Yes. Unless special scenarios such as technical constraints or academic study, you should always prioritize to purchase the latest version GB 5413.18-2010 even if the enforcement date is in future. Complying with the latest version means that, by default, it also complies with all the earlier versions, technically.

How to buy and download a true PDF of English version of GB 5413.18-2010?

A step-by-step guide to download PDF of GB 5413.18-2010_EnglishStep 1: Visit website https://www.ChineseStandard.net (Pay in USD), or https://www.ChineseStandard.us (Pay in any currencies such as Euro, KRW, JPY, AUD).
Step 2: Search keyword "GB 5413.18-2010".
Step 3: Click "Add to Cart". If multiple PDFs are required, repeat steps 2 and 3 to add up to 12 PDFs to cart.
Step 4: Select payment option (Via payment agents Stripe or PayPal).
Step 5: Customize Tax Invoice -- Fill up your email etc.
Step 6: Click "Checkout".
Step 7: Make payment by credit card, PayPal, Google Pay etc. After the payment is completed and in 9 seconds, you will receive 2 emails attached with the purchased PDFs and PDF-invoice, respectively.
Step 8: Optional -- Go to download PDF.
Step 9: Optional -- Click Open/Download PDF to download PDFs and invoice.
See screenshots for above steps: Steps 1~3    Steps 4~6    Step 7    Step 8    Step 9