GB 5009.8-2023 PDF EnglishUS$335.00 · In stock · Download in 9 seconds
GB 5009.8-2023: National food safety standard - Determination of fructose, glucose, sucrose, maltose and lactose in foods Delivery: 9 seconds. True-PDF full-copy in English & invoice will be downloaded + auto-delivered via email. See step-by-step procedure Status: Valid GB 5009.8: Historical versions
Similar standardsGB 5009.8-2023: National food safety standard - Determination of fructose, glucose, sucrose, maltose and lactose in foods---This is an excerpt. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.), auto-downloaded/delivered in 9 seconds, can be purchased online: https://www.ChineseStandard.net/PDF.aspx/GB5009.8-2023 GB NATIONAL STANDARD OF THE PEOPLE’S REPUBLIC OF CHINA National food safety standard - Determination of fructose, glucose, sucrose, maltose and lactose in foods Issued on. SEPTEMBER 06, 2023 Implemented on. MARCH 06, 2024 Issued by. National Health Commission of the People’s Republic of China; State Administration for Market Regulation. Table of ContentsForeword... 3 1 Scope... 4 2 Principle... 4 3 Reagents and materials... 4 4 Instruments and apparatuses... 6 5 Analysis steps... 6 6 Expression of analysis results... 8 7 Precision... 9 8 Others... 9 9 Principle... 9 10 Reagents and materials... 9 11 Instruments and apparatuses... 11 12 Test steps... 11 13 Expression of analysis results... 14 14 Precision... 14 15 Others... 14 16 Principle... 15 17 Reagents and solutions... 15 18 Instruments and apparatuses... 16 19 Analysis steps... 16 20 Expression of analysis results... 19 21 Precision... 21 22 Principle... 21 23 Reagents and materials... 21 24 Instruments and apparatuses... 22 25 Analysis steps... 22 26 Expression of analysis results... 24 27 Precision... 251 ScopeThis Standard specifies the methods for the determination of fructose, glucose, sucrose, maltose and lactose in foods. Method 1 - High performance liquid chromatography applies to the determination of fructose, glucose, sucrose, maltose and lactose in grains and grain products, milk and dairy products, fruits, vegetables and fruit and vegetable products, sweeteners, candies, beverages and infant foods. Method 2 - Ion chromatography applies to the determination of fructose, glucose, sucrose, maltose, and lactose in foods. Method 3 - Acid hydrolysis-Rhein-Eynon method applies to the determination of sucrose in foods. Method 4 - Rhine-Enon method applies to the determination of lactose in infant foods and dairy products. Method 1 - High performance liquid chromatography2 PrincipleThe fructose, glucose, sucrose, maltose and lactose in the sample are extracted, and then separated by high performance liquid chromatography column, detected by a differential refractive index detector or an evaporative light scattering detector, and quantified by the external standard method.3 Reagents and materialsUnless otherwise specified, all the reagents in this method are analytical reagents, and the water is grade-1 water specified by GB/T 6682. 3.1 Reagents 3.2 Preparation of reagents 3.3 Standards 3.4 Preparation of standard solutions 3.5 Materials 3.5.2 Syringe.4 Instruments and apparatuses4.1 High performance liquid chromatograph. equipped with a differential refractive index detector or an evaporative light scattering detector. 4.2 Analytical balance. the sensitivity is 1 mg and 10 mg. 4.3 Vortex mixer. 4.4 Centrifuge. speed ≥ 4 000 r/min. 4.5 Ultrasonic cleaner. 4.6 Sample crushing equipment. high-speed crusher. 4.7 Constant-temperature drying oven. 4.8 Constant-temperature water bath device.5 Analysis steps5.1 Sample pretreatment 5.1.2 Sample extraction 5.1.3 Purification For the above sample extract solution, use a filter paper to filter (discard the primary filtrate) or centrifuge to obtain the supernatant; then, use a 0.45 μm water-based membrane syringe to filter into a sample bottle for analysis by a high-performance liquid chromatograph. 5.2 Apparatus reference conditions Apparatus reference conditions are as below. 90 °C; nitrogen flow rate 2.5 L/min. 5.3 Preparation of the standard curve Inject the mixed standard working solution into the high-performance liquid chromatograph in order from low to high concentration, and measure the corresponding peak areas or peak heights of fructose, glucose, sucrose, maltose and lactose. For the differential refractive index detector, use the concentration of the standard working fluid as the abscissa and the peak area or peak height as the ordinate to draw a standard curve; 5.4 Determination of sample solution Inject the sample solution into the high-performance liquid chromatograph; qualitatively record the peak area or peak height of the target substance based on the retention time; obtain the concentrations of fructose, glucose, sucrose, maltose and lactose in the sample solution based on the standard curve. 5.5 Blank test Except that no sample is added, proceed according to the above steps.6 Expression of analysis resultsCalculate the contents of fructose, glucose, sucrose, maltose and lactose in the sample according to Formula (1).7 PrecisionThe absolute difference of 2 independent test results obtained under repeatability cannot exceed 10% of the arithmetic mean value.8 OthersWhen the weighing sample is 10 g and the fixed volume is 100 mL, the method detection limits for fructose, glucose, sucrose, maltose and lactose are all 0.2 g/100 g, and the quantification limits are all 0.5 g/100 g.9 PrincipleAfter extraction and purification, the fructose, glucose, sucrose, maltose and lactose in the sample are separated by an ion chromatography column, detected by a pulse ampere detector, and quantified by the external standard method.10 Reagents and materialsUnless otherwise specified, all the reagents in this method are analytical reagents, and the water is grade-1 water specified by GB/T 6682. 10.1 Reagents 10.1.1 Sodium hydroxide (NaOH). chromatographic pure. 10.1.2 Glacial acetic acid (CH3COOH). 10.2 Preparation of reagents 10.3 Standards 10.3.1 Fructose (C6H12O6, CAS number. 57-48-7). purity ≥99%, or standard material certified by the country and awarded a standard material certificate. 10.3.2 Glucose (C6H12O6, CAS number. 50-99-7). purity ≥99%, or standard material certified by the country and awarded a standard material certificate. 10.4 Preparation of standard solution 10.5 Materials 10.5.1 0.45 μm water-based membrane syringe filter (except cellulose membrane). 10.5.2 Purification column. C18 solid-phase extraction cartridge (1.0 mL) or one with equivalent performance. 10.5.3 Syringe.11 Instruments and apparatuses11.1 Ion chromatograph. equipped with pulse ampere detector. 11.2 Sample crushing equipment. high-speed crusher. 11.3 Ultrasonic cleaner. 11.4 Analytical balance. sensitivity 1 mg. 11.5 Vortex mixer. 11.6 Centrifuge. speed ≥ 4 000 r/min. 11.7 Constant-temperature drying oven. 11.8 Constant-temperature water bath device.12 Test steps12.1 Sample pretreatment 12.1.1 Sample preparation Take an appropriate amount of representative sample; for drinks and other liquid homogeneous samples, shake directly; for non-uniform samples, homogenize or crush evenly; for frozen drinks, melt at room temperature, stir thoroughly and, if necessary, heat and stir in a water batch at 30 °C ~ 40 °C; for sauces, grind or homogeneously mix; for chocolate, heat and melt in a water bath at 50 °C ~ 60 °C, and stir thoroughly while it is hot. 12.1.2 Sample extraction 12.1.3 Sample purification The sample extraction solution can be diluted by an appropriate multiple according to the target sugar content in the sample. When detecting lactose in infant formula milk powder, dilute it 1 000 times and then purify it; when detecting high sugar content in honey and candy, dilute it 500 times and then purify it. After filtering or centrifuging the above sample extraction solution or dilution solution to obtain the supernatant, pass it through a 0.45 μm aqueous membrane syringe filter and a C18 solid phase extraction cartridge (1.0 mL) in sequence; discard the first 3 mL; collect subsequent eluates for testing. 12.2 Apparatus reference conditions Apparatus reference conditions are as below. 12.3 Drawing of standard working curve Inject the mixed standard working solution into the ion chromatograph in order from low to high concentration, and measure the corresponding peak areas of fructose, glucose, sucrose, maltose and lactose. Taking the concentrations of fructose, glucose, sucrose, maltose and lactose in the standard working solution as the abscissa and the peak area as the ordinate, draw the standard curves, respectively. See Appendix B for the ion chromatograms of standard solutions of fructose, glucose, sucrose, maltose and lactose. 12.4 Determination of sample solution Inject the sample solution into the ion chromatograph, identify it based on the retention time, record the peak area, and obtain the concentrations of fructose, glucose, sucrose, maltose and lactose in the sample solution based on the standard curve. 12.5 Blank test Except that no sample is added, proceed according to the above steps.13 Expression of analysis resultsCalculate the contents of fructose, glucose, sucrose, maltose and lactose in the sample according to Formula (2).14 PrecisionThe absolute difference of 2 independent test results obtained under repeatability cannot exceed 10% of the arithmetic mean value. ......Source: Above contents are excerpted from the full-copy PDF -- translated/reviewed by: www.ChineseStandard.net / Wayne Zheng et al. 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