GB 5009.34-2022 PDF English
GB 5009.34: Historical versions
| Standard ID | USD | BUY PDF | Delivery | Standard Title (Description) | Status |
| GB 5009.34-2022 | 215 | Add to Cart | Auto, 9 seconds. | National food safety standard - Determination of sulfur dioxide in foods | Valid |
| GB 5009.34-2016 | 70 | Add to Cart | Auto, 9 seconds. | National Food Safety Standard -- Determination of sulfur dioxide in food stuffs | Obsolete |
| GB/T 5009.34-2003 | 70 | Add to Cart | Auto, 9 seconds. | Determination of sulphite in foods | Obsolete |
| GB/T 5009.34-1996 | 199 | Add to Cart | 2 days | Method for determination of sulphite in foods | Obsolete |
| GB 5009.34-1985 | 199 | Add to Cart | 2 days | Method for determination of sulphite in foods | Obsolete |
Similar standards
GB 5009.34-2022: National food safety standard - Determination of sulfur dioxide in foods
---This is an excerpt. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.), auto-downloaded/delivered in 9 seconds, can be purchased online: https://www.ChineseStandard.net/PDF.aspx/GB5009.34-2022NATIONAL STANDARD OF THE PEOPLE’S REPUBLIC OF CHINA National food safety standard - Determination of sulfur dioxide in foods Issued on. JUNE 30, 2022 Implemented on. DECEMBER 30, 2022 Issued by. National Health Commission of the People's Republic of China; Standardization Administration of the People's Republic of China.
Table of Contents
Foreword... 3 1 Scope... 4 2 Principle... 4 3 Reagents and materials... 4 4 Instruments and equipment... 5 5 Analysis steps... 5 6 Presentation of analysis results... 7 7 Precision... 7 8 Detection limit and quantification limit... 7 9 Principle... 8 10 Reagents and materials... 8 11 Instruments and equipment... 9 12 Analysis steps... 9 13 Presentation of analysis results... 10 14 Precision... 11 15 Detection limit and quantification limit... 11 16 Principle... 11 17 Reagents and materials... 12 18 Instruments and equipment... 12 19 Analysis steps... 12 20 Presentation of analysis results... 14 21 Precision... 14 22 Detection limit and quantification limit... 14 Annex A Schematic diagram of distillation device for acid-base titration... 16 Annex B Schematic diagram of steam distillation device... 17 Annex C Typical spectrum of sulfur dioxide standard working solution... 18Foreword
This document replaces GB 5009.34-2016 "National Food Safety Standard - Determination of sulfur dioxide in food stuffs". Compared with GB 5009.34-2016, the main changes in this document are as follows. - Modify the original titration method to acid-base titration method. - Add spectrophotometry, ion chromatography. National food safety standard - Determination of sulfur dioxide in foods1 Scope
This document specifies the determination method of sulfur dioxide in foods. Method One. Acid-base titration method is applicable to the determination of sulfur dioxide in foods. Method Two is Spectrophotometry. Direct extraction method is suitable for the determination of sulfur dioxide in white sugar and white sugar products, starch and starch products and raw wet flour products. Nitrogen-filled steam extraction method is suitable for the determination of sulfur dioxide in wine and brown sugar. Method Three. Ion chromatography is applicable to the determination of sulfur dioxide in foods. Method One -- Acid-base titration method2 Principle
Use nitrogen-filled steaming method to treat the specimen. After the specimen is acidified, under heating conditions, a series of substances such as sulfites release sulfur dioxide. Use hydrogen peroxide solution to absorb the distillate. Sulfur dioxide is dissolved in the absorption liquid and oxidized to form sulfuric acid. Use standard sodium hydroxide solution to titrate. Calculate the content of sulfur dioxide in the specimen according to the consumption of sodium hydroxide standard solution.3 Reagents and materials
Unless otherwise stated, the reagents used in this method are all analytically pure; the water is grade three water specified in GB/T 6682. 3.1 Reagents 3.1.1 Hydrogen peroxide (H2O2). 30%. 3.1.2 Absolute ethanol (C2H5OH). 3.1.3 Sodium Hydroxide (NaOH). 3.1.4 Methyl red (C15H15N3O2). 3.1.5 Hydrochloric acid (HCl) (ρ20=1.19g/mL). 3.1.6 Nitrogen (purity >99.9%). 3.2 Reagent preparation 3.2.1 Hydrogen peroxide solution (3%). Measure 100mL of hydrogen peroxide of which its mass fraction is 30%. Add water to dilute to 1000mL. Prepare it when it is needed. 3.2.2 Hydrochloric acid solution (6mol/L). Measure 50mL of hydrochloric acid (ρ20=l.19g/mL). Pour slowly into 50mL of water. Stir while pouring. 3.2.3 Methyl red ethanol solution indicator (2.5g/L). Weigh 0.25g of methyl red indicator. Dissolve in 100mL of absolute ethanol. 3.3 Preparation of standard solution4 Instruments and equipment
4.1 Glass nitrogen-filled steamer. 500mL or 1000mL. Equip it with electric heating jacket, nitrogen source and gas flow meter, or equivalent steaming equipment. See Annex A for the schematic diagram of the device. 4.2 Electronic balance. Resolution is 0.01g. 4.3 10mL semi-micro-burettes and 25mL burettes. 4.4 Grinder. 4.5 Tissue masher.5 Analysis steps
5.1 Specimen pretreatment 5.1.1 Liquid specimen Take specimens of beer, wine, fruit wine, other fermented wine, prepared wine, and beverages. The sampling volume shall be greater than 1L. For packaging specimens such as bagged specimens and bottled specimens, at least 3 packages (same batch or number) shall be collected. Put all liquids in one container. Mix well. Seal and mark for testing. 5.1.2 Solid specimen Take specimens of grain processed products, solid condiments, biscuits, potato products, candy products (including chocolate and products), substitute tea, pickled vegetables, dried vegetable products, edible mushroom products, other vegetable products, candied fruit, dried fruit products, roasted seeds and nuts and nut products (baked, fried, other), sugar, dried aquatic products, cooked animal aquatic products, edible starch, starch products, starch sugar, non-fermented soy products, vegetables, fruits, seawater products, raw and dried nuts and seeds. 5.1.3 Semi-fluid specimen For packaging specimens such as bagged specimens and bottled specimens, at least 3 packages (same lot or number) need to be collected. 5.2 Specimen determination Take 20g~100g of solid or semi-fluid specimen (to the nearest of 0.01g; the sampling amount depends on the content). Take 20mL(g)~200mL(g) of liquid specimen. Place the weighed specimen in the round bottom flask A in Figure A.1.Add 200mL~500mL of water. Install the device. Turn on the switch of the reflux condenser to supply water (condensate temperature < 15°C). Place the glass tube connected to the port E at the upper end of the condenser tube at the bottom of the 100mL conical flask. Add 50mL of 3% hydrogen peroxide solution to the conical flask as the absorption solution (the end of the glass tube shall be below the liquid level of the absorption solution). Add 3 drops of 2.5g/L methyl red ethanol solution indicator to the absorption solution.6 Presentation of analysis results
The content of sulfur dioxide in the specimen is calculated according to formula (1).7 Precision
The absolute difference between the results of two independent determinations obtained under repeatability conditions shall not exceed 10% of the arithmetic mean.8 Detection limit and quantification limit
When using 0.01mol/L sodium hydroxide titrant, when the solid or semi-fluid sampling weight is 35g, the detection limit is 1mg/kg, the quantification limit is 10mg/kg.9 Principle
The sample is directly soaked in methyl acetate buffer absorbent or nitrogen-filling with acid and steamed-released sulfur dioxide is absorbed by the formaldehyde solution. Generate stable hydroxymethanesulfonic acid addition compound.10 Reagents and materials
Unless otherwise stated, the reagents used in this method are all analytically pure, and the water is grade three water specified in GB/T 6682. 10.1 Reagents 10.1.1 Ammonium sulfamate (H6N2O3S). 10.1.2 Disodium EDTA (C10H14N2Na2O8). 10.1.3 Formaldehyde (CH2O). 36%~38%; there shall be no polymer (no precipitation and no separation of the solution). 10.1.4 Potassium hydrogen phthalate (KHC8H4O4). 10.2 Reagent preparation 10.2.1 Sodium hydroxide solution (1.5mol/L). Weigh 6.0g of NaOH (3.1.3). Dissolve in water and dilute to 100mL. 10.2.2 Disodium EDTA solution (0.05mol/L). Weigh 1.86g of disodium ethylenediaminetetraacetate (abbreviated as EDTA-2Na). Dissolve in water and dilute to 100mL. 10.2.5 Pararosaniline hydrochloride solution (0.5g/L). Measure 25.0mL of 2% pararosaniline hydrochloride solution. Respectively add 30mL of phosphoric acid and 12mL of hydrochloric acid (3.1.5). Use water to dilute to 100mL. Shake well. Place for 24h for future use (store in an airtight seal away from light). 10.2.6 Ammonium sulfamate solution (3g/L). Weigh 0.30g of ammonium sulfamate (H6N2O3S). Dissolve in water and dilute to 100mL. 10.3 Standard product Sulfur dioxide standard solution (100μg/mL). With national certification and granted a standard substance certificate. 10.4 Preparation of standard solution Sulfur dioxide standard solution (10μg/mL). Accurately pipette 5.0mL of sulfur dioxide standard solution (100μg/mL). Use formaldehyde buffer absorption solution to set volume to 50mL. Prepare it when it is needed.11 Instruments and equipment
11.1 Glass nitrogen-filled distiller. 500mL or 1000mL, or equivalent steaming equipment. See Annex A for the schematic diagram of the device. 11.2 UV-visible spectrophotometer.12 Analysis steps
12.1 Specimen preparation Same with 5.1. 12.2 Specimen processing 12.2.1 Direct extraction method Weigh about 10g of solid specimen (to the nearest of 0.01g). Add 100mL of formaldehyde buffered absorption solution. Vibrate and soak for 2h. Filter. Take the filtrate for testing. Conduct blank test at the same time. 12.3 Preparation of standard curve Respectively and accurately measure 0.00mL, 0.20mL, 0.50mL, 1.00mL, 2.00mL, 3.00mL of sulfur dioxide standard solution (equivalent to 0.0μg, 2.0μg, 5.0μg, 10.0μg, 20.0μg, 30.0μg sulfur dioxide). 12.4 Determination of specimen solution According to the sulfur dioxide content in the specimen, pipette 0.50mL~10.00mL of specimen solution. Placed in a 25mL stoppered test tube. Follow the operation of 12.3 "Add formaldehyde buffer absorption solution to 10.00mL...". Conduct the blank test at the same time. ......Source: Above contents are excerpted from the full-copy PDF -- translated/reviewed by: www.ChineseStandard.net / Wayne Zheng et al.
