GB 5009.140-2023 PDF EnglishUS$140.00 · In stock · Download in 9 seconds
GB 5009.140-2023: National food safety standard - Determination of acesulfame K in food Delivery: 9 seconds. True-PDF full-copy in English & invoice will be downloaded + auto-delivered via email. See step-by-step procedure Status: Valid GB 5009.140: Historical versions
Similar standardsGB 5009.140-2023: National food safety standard - Determination of acesulfame K in food---This is an excerpt. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.), auto-downloaded/delivered in 9 seconds, can be purchased online: https://www.ChineseStandard.net/PDF.aspx/GB5009.140-2023GB NATIONAL STANDARD OF THE PEOPLE’S REPUBLIC OF CHINA National food safety standard - Determination of acesulfame potassium in food Issued on. SEPTEMBER 06, 2023 Implemented on. MARCH 06, 2024 Issued by. National Health Commission of the People’s Republic of China; State Administration for Market Regulation. Table of ContentsForeword... 3 1 Scope... 4 2 Principle... 4 3 Reagents and materials... 4 4 Instruments and equipment... 6 5 Analysis procedure... 6 6 Expression of analysis results... 8 7 Precision... 9 8 Others... 9 Annex A Liquid chromatogram of acesulfame potassium standard solutions... 10ForewordThis Standard replaces GB 5009.140-2003 “National food safety standard - Determination of acesulfame potassium in food”. Compared with GB 5009.140-2003, the main changes in this Standard are as follows. - MODIFY the name of the standard TO “National food safety standard - Determination of acesulfame potassium in food”; - EXPAND the scope of the method; - MODIFY the sample pretreatment method and instrument reference conditions; - ADD the detection limit and quantification limit of the method; - DELETE the content related to the determination of saccharin sodium. National food safety standard - Determination of acesulfame potassium in food1 ScopeThis Standard specifies the method for the determination of acesulfame potassium in food. This Standard applies to the determination of acesulfame potassium in milk and dairy products, frozen drinks, fruit products, vegetable products, edible fungi and algae, legume products, nuts and seeds, candy, grain products, baked goods, condiments, beverages, alcoholic beverages and jelly.2 PrincipleThe acesulfame potassium in the sample is extracted with water, subjected to protein precipitation using potassium ferrocyanide/zinc acetate solution or purified with a neutral alumina solid-phase extraction column depending on the sample type, diluted to the mark with water, filtered through a microporous membrane, separated by liquid chromatography, detected with a UV detector or a diode array detector, subjected to qualitative identification based on the retention time, and quantified by external standard method.3 Reagents and materialsUnless otherwise stated, the reagents used in this method are of analytical regent, and the water is Grade 1 water specified in GB/T 6682. 3.1 Reagents 3.2 Preparation of reagents 3.2.1 Potassium ferrocyanide solution (92 g/L). Weigh 106 g of potassium ferrocyanide, add water to dissolve, and dilute to 1000 mL. 3.2.2 Zinc acetate solution (183 g/L). Weigh 219 g of zinc acetate, dissolve in a small amount of water, add 32 mL of glacial acetic acid, and dilute to 1000 mL with water. 3.3 Reference material Acesulfame potassium reference material (C4H4KNO4S, CAS number. 55589-62-3), purity > 99.0 %, or a reference material certified by the country and awarded a reference material certificate. 3.4 Preparation of standard solutions 3.4.1 Acesulfame potassium standard stock solution (1000 mg/L). Accurately weigh 100 mg of acesulfame potassium reference material (accurate to 0.1 mg), dissolve in water and dilute to 100 mL, and mix well. Store at 0 ℃ ~ 4 ℃, the validity period is 6 months. 3.5 Materials 3.5.1 Neutral alumina solid-phase extraction column (6 mL, 500 mg), activated with 10 mL of acetonitrile before use, and then balanced with 10 mL of water. 3.5.2 Water phase microporous filter membrane. 0.45 μm. 3.5.3 Plastic centrifuge tube. 50 mL.4 Instruments and equipment4.1 High-performance liquid chromatograph. equipped with a UV detector or a diode array detector. 4.6 Constant temperature water bath. 4.7 Ultrasonic generator. 4.8 Solid-phase extraction device. 4.9 Homogenizer.5 Analysis procedure5.1 Preparation of samples Homogeneous liquid samples are mixed directly, and gas-containing samples are transferred to a beaker before use and degassed in a 50 °C water bath for 10 minutes or ultrasonic for 5 minutes; non-homogeneous liquid and semi-solid samples are homogenized with a homogenizer; solid samples are ground thoroughly with a food grinder and mix well. 5.2 Treatment of samples 5.2.1 Beverages (except protein drinks), fruit products, vegetable products Weigh about 5 g (accurate to 0.001 g) of the sample in a 50 mL centrifuge tube, add 20 mL of water, vortex to mix well, sonicate for 20 minutes, centrifuge at 10000 r/min for 5 minutes, and transfer the supernatant to a 50 mL volumetric flask. 5.2.2 Other samples Weigh 5 g (accurate to 0.001 g) of the sample in a 50 mL centrifuge tube, add 20 mL of water, vortex to mix well, and sonicate for 20 minutes. 5.3 Instrument reference conditions 5.3.1 Chromatographic column. C18 column (column length 250 mm, inner diameter 4.6 mm, particle size 5 μm), or a chromatographic column with equivalent performance. 5.3.3 Flow rate. 1.0 mL/min. 5.3.4 Column temperature. 30 ℃. 5.4 Plotting of standard curve Time Phase A Phase B Inject the standard series working solutions into the high-performance liquid chromatograph in order from low to high concentration, measure the corresponding peak area, and plot the standard curve with the concentration of acesulfame potassium in the standard working solutions as the abscissa and the peak area as the ordinate. For the liquid chromatogram of acesulfame potassium standard solutions, see Figure A.1 in Annex A. 5.5 Determination of sample solution Inject the sample solution into the high-performance liquid chromatograph, make qualitative identification based on the retention time, measure the peak area, and obtain the concentration of acesulfame potassium in the sample solution according to the standard curve.6 Expression of analysis resultsThe content of acesulfame potassium in the sample is calculated according to formula (1).7 PrecisionThe absolute difference between two independent determination results obtained under repeatability conditions shall not exceed 10 % of the arithmetic mean.8 OthersWhen the sampling volume is 5 g and the constant volume is 50 mL, the detection limit of acesulfame potassium is 0.0006 g/kg, and the quantification limit is 0.002 g/kg.Annex ALiquid chromatogram of acesulfame potassium standard solutions The liquid chromatogram of acesulfame potassium standard solutions is shown in A.1. Figure A.1 -- Liquid chromatogram of acesulfame potassium standard solutions (10.0 mg/L) Acesulfame potassium ......Source: Above contents are excerpted from the full-copy PDF -- translated/reviewed by: www.ChineseStandard.net / Wayne Zheng et al. Tips & Frequently Asked Questions:Question 1: How long will the true-PDF of English version of GB 5009.140-2023 be delivered?Answer: The full copy PDF of English version of GB 5009.140-2023 can be downloaded in 9 seconds, and it will also be emailed to you in 9 seconds (double mechanisms to ensure the delivery reliably), with PDF-invoice.Question 2: Can I share the purchased PDF of GB 5009.140-2023_English with my colleagues?Answer: Yes. The purchased PDF of GB 5009.140-2023_English will be deemed to be sold to your employer/organization who actually paid for it, including your colleagues and your employer's intranet.Question 3: Does the price include tax/VAT?Answer: Yes. 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