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GB 31645-2018 PDF English

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GB 31645-2018: National food safety standard -- Collagen peptide
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GB 31645-201885 Add to Cart Auto, 9 seconds. National food safety standard -- Collagen peptide Valid

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GB 31645-2018: National food safety standard -- Collagen peptide

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GB NATIONAL STANDARD OF THE PEOPLE’S REPUBLIC OF CHINA National Food Safety Standard - Collagen Peptide Issued on. JUNE 21, 2018 Implemented on. DECEMBER 21, 2018 Issued by. National Health Commission of the People’s Republic of China; State Administration for Market Regulation.

Table of Contents

1 Scope... 3 2 Terms and Definitions... 3 3 Technical Requirements... 3 Appendix A... 6 National Food Safety Standard - Collagen Peptide

1 Scope

This Standard is applicable to collagen peptide products for the purpose of food processing.

2 Terms and Definitions

2.1 Collagen Peptide Collagen peptide is a product that takes fresh animal tissues (including skin, bone, muscle, tendon and scale, etc.) that contain sufficient collagen as the raw material. It is produced through extraction, hydrolysis and refining; its molecular mass is lower than 10,000.

3 Technical Requirements

3.1 Raw Material Requirements 3.1.1 Raw materials that can be adopted. 3.1.2 Raw materials that are prohibited. 3.2 Sensory Requirements Sensory requirements shall comply with the stipulation in Table 1 below. 3.3 Physical and Chemical Indicators Physical and chemical indicators shall comply with the stipulation in Table 2 below. 3.5 Microorganism Limit Microorganism limit shall comply with the stipulation in Table 4 below.

Appendix A

Test Method of Proportion of Collagen Peptide with Relative Molecular Mass Less Than 10,000 (High-performance size exclusion chromatography) A.1 Method Summary High-performance size exclusion chromatography shall be adopted in determination. Specifically speaking, adopt porous filler as the stationary phase; separate in accordance with the difference in molecular volume of the sample components; test under the condition of 220 nm ultraviolet absorption wavelength of peptide bond. Adopt exclusive data processing software (namely, GPC software) for the determination of relative molecular mass distribution; process the chromatogram and the data of standard substance and the sample. In accordance with relative molecular mass correction curve equation, calculate the relative molecular mass and distribution range of collagen peptide. A.2 Reagents A.2.1 Acetonitrile. chromatographic purity. A.2.2 Trifluoroacetate. analytical purity. A.2.3 Water. Grade-1 water stipulated in GB/T 6682. A.2.4 Standard substances applied in relative molecular mass correction curve. A.3 Instruments and Equipment A.3.1 High-performance liquid chromatograph. equipped with UV detector and chromatography workstation that contains GPC data processing software. A.3.2 Mobile phase vacuum filtration degasser. A.3.3 Ultrasonic oscillator. A.3.4 Analytical balance. division value. 0.0001 g. A.4 Chromatographic conditions and System Adaptability Experiment Respectively use mobile phase to prepare peptide standard solutions of the above- mentioned different relative molecular mass at the concentration of around 1.0 g/L. Use polytetrafluoroethylene (PTFE) or nylon filter membrane (aperture. 0.2 μm ~ 0.5 μm) to filter it. Then, respectively inject it; obtain the chromatogram of series standard substance. Use the logarithm of relative molecular mass (lgMW) to draw the retention time or conduct linear regression. Thus, obtain the relative molecular mass correction curve and its equation. A.6 Sample Preparation Use weighing paper to weigh-take around 125.0 mg of sample; transfer it into a 25 mL volumetric flask. Use mobile phase to fixate it at a constant volume. Start ultrasonic oscillation for 10 min, so that the sample can be thoroughly dissolved and mixed. Use polytetrafluoroethylene (PTFE) or nylon filter membrane (aperture. 0.2 μm ~ 0.5 μm) to filter it. Reserve the filtrate for determination. ......

Source: Above contents are excerpted from the full-copy PDF -- translated/reviewed by: www.ChineseStandard.net / Wayne Zheng et al.
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