GB 31604.52-2021 PDF EnglishUS$260.00 · In stock · Download in 9 seconds
GB 31604.52-2021: National food safety standard - Food-contact materials and articles - Determination of migration of primary aromatic amine Delivery: 9 seconds. True-PDF full-copy in English & invoice will be downloaded + auto-delivered via email. See step-by-step procedure Status: Valid
Similar standardsGB 31604.52-2021: National food safety standard - Food-contact materials and articles - Determination of migration of primary aromatic amine---This is an excerpt. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.), auto-downloaded/delivered in 9 seconds, can be purchased online: https://www.ChineseStandard.net/PDF.aspx/GB31604.52-2021 GB NATIONAL STANDARD OF THE PEOPLE’S REPUBLIC OF CHINA National food safety standard - Food contact material and products - Determination of migration of primary aromatic amine Issued on. FEBRUARY 22, 2021 Implemented on. AUGUST 22, 2021 Issued by. National Health Commission of PRC; State Administration for Market Regulation. Table of Contents1 Scope... 3 2 Principles... 3 3 Reagents and materials... 3 4 Instruments and equipment... 5 5 Analytical procedures... 5 6 Expression of analysis results... 8 7 Precision... 10 8 Others... 10 Appendix A The names, CAS numbers, molecular formulas of 29 aromatic primary amines... 11 Appendix B Mass spectrometry reference conditions... 12 Appendix C Chromatograms of liquid chromatography-mass spectrometry/mass total ion current (TIC) AND chromatograms of multiple reaction monitoring (MRM) of 29 aromatic primary amines... 16 National food safety standard - Food contact material and products - Determination of migration of primary aromatic amine1 ScopeThis standard specifies the method for determining the migration of 29 aromatic primary amines, in food contact materials and products. This standard applies to the determination of the migration of aromatic primary amines, in food contact materials and products.2 PrinciplesAfter the migration test of food contact materials and products, according to GB 31604.1 and GB 5009.156, they are detected by liquid chromatography-mass spectrometry/mass spectrometry. Among them, the food simulants, including 4% acetic acid, 10% ethanol, 20% ethanol, 50% ethanol, AND the alternative chemical solvents, that is, 95% ethanol, are filtered and injected directly. The alternative chemical solvent isooctane is extracted by methanol-water solution; then the water layer is filtered and injected. Use the peak area external standard method for quantification.3 Reagents and materialsThe water is the grade 1 water, which is specified in GB/T 6682. 3.1 Reagents 3.2 Reagent preparation 3.2.1 Acidic and alcoholic food simulants and alternative chemical solvents. Prepared according to GB 5009.156. 3.2.2 The 0.1% formic acid. Take 1 mL of formic acid (3.1.3) in a volumetric flask; add water to make the volume reach to 1 L. 3.2.3 Methanol-water extract. Measure 50 mL of methanol (3.1.2) AND 50 mL of water. Pipette 0.4 mL of glacial acetic acid (3.1.5). Mix it uniformly. 3.3 Standard substances The 29 kinds of aromatic primary amines. Standard substances (see appendix A), purity ≥ 98%; or standard substances that have been certified by the country and awarded a standard material certificate. 3.4 Preparation of standard solution 3.4.1 The 29 kinds of standard stock solutions of aromatic primary amine (1000 mg/L). Accurately weigh 25 mg (accurate to 0.1 mg) of each of 29 kinds of aromatic primary amine standard substances. Use methanol to dissolve them, respectively. Transfer it into a 25 mL brown volumetric bottle. Use methanol to dilute it to the mark. Seal and store it in a 4 °C refrigerator in the dark. The shelf life is 1 month. 3.4.4 Preparation of standard working solution 3.4.4.1 Isooctane standard working solution Accurately pipette 30 µL, 50 µL, 100 µL, 300 µL, 500 µL of the standard intermediate solution B of aromatic primary amine (3.4.3), in a 10 mL volumetric flask. Use isooctane to make the volume reach to the mark, to obtain the standard working solution, which has an aromatic primary amine concentration of 3.0 µg/L, 5.0 µg/L, 10.0 µg/L, 30.0 µg/L, 50.0 µg/L, respectively. 3.4.4.2 Standard working solutions for other food simulants and alternative chemical solvents Accurately pipette 30 µL, 50 µL, 100 µL, 300 µL, 500 µL of the standard intermediate solution A (3.4.2) of aromatic primary amine, in a 10 mL volumetric flask.4 Instruments and equipment4.1 Liquid chromatography tandem mass spectrometer. Electrospray ion source (ESI) 4.2 Analytical balance. Sensitivity is 0.0001 g. 4.3 Micro syringe. 10 µL, 100 µL, 1000 µL. 4.5 Vortex mixer. 4.6 Needle type nylon filter. Pore size 0.22 µm.5 Analytical procedures5.1 Preparation of food simulants 5.1.1 General The migration test of this standard uses 4% acetic acid, 10% ethanol, 20% ethanol, 50% ethanol food simulants, alternative chemical solvent (95% ethanol), isooctane. Vegetable oil is not suitable for the determination of the migration of aromatic primary amines. 5.1.2 Migration test The condition selection and operation steps of the migration test are in accordance with the provisions of GB 31406.1 and GB 5009.156. 5.1.3 Preparation of test solution 5.1.3.3 Preparation of blank test solution Food simulants and alternative chemical solvents, which are not in contact with food contact materials and products, are treated in accordance with 5.1.3.1 and 5.1.3.2, to obtain a blank test solution. 5.2 Instrument reference conditions 5.2.1 Liquid chromatography conditions The liquid chromatography conditions are as follows. 5.2.2 Mass spectrometry conditions The mass spectrometry conditions are as follows. 5.3 Drawing of standard curve According to the instrument reference conditions listed in 5.2, the standard working solution is determined. Use the concentration of the aromatic primary amine in the standard working solution as the abscissa, the corresponding quantitative ion peak area as the ordinate, to draw the standard curve, to obtain the linear equation. 5.4 Determination of test solution 5.4.1 Qualitative determination Determine the food simulant sample solution (5.1.3) and standard working solution, according to the instrument reference condition (5.2). If the deviation of the retention time of the mass chromatographic peak of the food simulant sample solution and the standard solution is within ±2.5%; 5.4.2 Quantitative determination Inject the test solution and the blank test solution (5.1.3) into the liquid chromatography-mass spectrometer/mass spectrometer, to obtain the peak area.6 Expression of analysis results6.1 Calculation of the specific migration of a single aromatic primary amine in food contact materials and products of non-sealed products (expressed in mg/kg) 6.2 Calculation of specific migration of single aromatic primary amine in food contact materials and products of sealed products (expressed in mg/kg) 6.3 Calculation of specific migration of single aromatic primary amine in food contact materials and products of sealed products (expressed in mg/piece)7 PrecisionThe absolute difference between two independent determination results, which are obtained under repeatability conditions, shall not exceed 10% of their arithmetic mean.8 OthersThis method has a detection limit of 1.0 µg/kg AND a quantification limit of 3.0 µg/kg, for a single aromatic primary amine in food simulants, including 4% acetic acid, 10% ethanol, 20% ethanol, 50% ethanol, alternative chemical solvents (95% ethanol), isooctane. ......Source: Above contents are excerpted from the full-copy PDF -- translated/reviewed by: www.ChineseStandard.net / Wayne Zheng et al. 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