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GB 20287-2006: Microbial inoculants in agriculture Delivery: 9 seconds. True-PDF full-copy in English & invoice will be downloaded + auto-delivered via email. See step-by-step procedure Status: Valid
Similar standardsGB 20287-2006: Microbial inoculants in agriculture---This is an excerpt. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.), auto-downloaded/delivered in 9 seconds, can be purchased online: https://www.ChineseStandard.net/PDF.aspx/GB20287-2006GB NATIONAL STANDARD OF THE PEOPLE’S REPUBLIC OF CHINA ICS 65.100.01 B 13 Microbial inoculants in agriculture Issued on. MAY 25, 2006 Implemented on. SEPTEMBER 01, 2006 Issued by. General Administration of Quality Supervision, Inspection and Quarantine of PRC; Standardization Administration of PRC. Table of ContentsForeword... 3 1 Scope... 4 2 Normative references... 4 3 Terms and definitions... 4 4 Product classification... 5 5 Requirements... 5 5.1 Bacteria species... 5 5.2 Product appearance (sensory)... 5 5.3 Product technical index... 5 6 Test method... 7 6.1 Instrument and equipment... 7 6.2 Reagents... 7 6.3 Testing of product parameters... 7 7 Inspection rules... 12 7.1 Sampling... 12 7.2 Inspection classification... 13 7.3 Judgment rules... 14 8 Packaging, marking, transportation, storage... 14 8.1 Packaging... 14 8.2 Markings... 15 8.3 Transportation... 16 8.4 Storage... 16 Appendix A (Normative) Commonly used testing medium... 17 Appendix B (Informative) Commonly used stains... 22 Appendix C (Normative) Dilution method (MPN 5-tube method)... 24 Appendix D (Normative) Determination of enzyme activity... 26ForewordClauses 5.1, 5.3, Chapter 8 of this standard are mandatory clauses. The rest are recommended clauses. Appendix A, Appendix C, Appendix D of this standard are normative appendixes. Appendix B is an informative appendix. This standard was proposed by AND shall be under the jurisdiction of the Ministry of Agriculture of the People's Republic of China. Drafting organizations of this standard. Microbial Fertilizer Quality Supervision, Inspection and Testing Center of Ministry of Agriculture, Soil and Fertilizer Research Institute of Chinese Academy of Agricultural Sciences. The main drafters of this standard. Shen Delong, Li Jun, Jiang Xin, Zhang Yujie, Li Li, Feng Ruihua, Cao Fengming, Yang Xiaohong, Guan Dawei. Microbial inoculants in agriculture1 ScopeThis standard specifies the terms and definitions, product classification, requirements, test methods, inspection rules, packaging, marking, transportation, storage of microbial inoculants in agriculture (i.e., microbial inoculants). This standard applies to microbial inoculum products in agriculture.2 Normative referencesThe provisions in following documents become the provisions of this Standard through reference in this Standard. For the dated references, the subsequent amendments (excluding corrections) or revisions do not apply to this Standard; however, parties who reach an agreement based on this Standard are encouraged to study if the latest versions of these documents are applicable. For undated references, the latest edition of the referenced document applies. GB/T 1250-1989 Rules for expression and judgement of limiting values GB/T 8170 Rules of rounding off for numerical values & expression and judgement of limiting values GB 18877-2002 Organic-inorganic compound fertilizers GB/T 19524.1 Determination of fecal coliforms in fertilizers GB/T 19524.2 Determination of mortality of ascarid egg in fertilizers QB/T 1803-1993 General methods of determination for industrial enzymes3 Terms and definitionsThe following terms and definitions apply to this standard. 3.1 Microbial inoculants in agriculture The live bacteria preparations, which are processed from target microorganisms (effective bacteria), through industrialized production and propagation.4 Product classificationAccording to the types or functional characteristics of the contained microorganisms, it can be divided into rhizobia inoculants, nitrogen-fixing bacteria inoculants, phosphate-dissolving microbial inoculants, silicate microbial inoculants, photosynthetic bacteria inoculants, decomposing agent for organic materials, growth-promoting bacteria, mycorrhizal inoculants, bioremediation inoculants, etc.5 Requirements5.1 Bacteria species The microbial strains, which are used in production, shall be safe and effective. The producer shall provide the classification and identification report of the bacterial species, including complete information such as the scientific name of the genera and species, morphology, 5.2 Product appearance (sensory) Powder products shall be loose. Granular products shall have no obvious mechanical impurities, be uniform in size, have water absorption. 5.3 Product technical index 5.3.1 The technical index of agricultural microbial inoculants products is as shown in Table 1, wherein the technical index of organic material decomposing agents are implemented in accordance with Table 2. 5.3.2 See Table 3 for harmlessness index of the microbial inoculants in agriculture.6 Test method6.1 Instrument and equipment 6.1.1 Biological microscope. 6.1.2 Constant temperature incubator. 6.1.3 Constant temperature drying oven. 6.1.4 Ultra-clean workbench or clean room. 6.1.5 Electronic balance (or precision balance). 6.1.6 Shaker. 6.1.7 Steam sterilization pot. 6.1.8 Test sieve. 6.1.9 Acidity meter. 6.2 Reagents 6.3 Testing of product parameters 6.3.1 Determination of appearance (sensory) Take a small amount of samples. Place them in a white enamel tray (or white plastic palette). Carefully observe the color, shape, texture of the sample. 6.3.2 Determination of effective viable bacteria count Use the plate counting method. Select an appropriate medium, according to the type of microorganisms tested. If the dilution method [Most Probable Number (MPN) 5 pipe method] is used, it is performed in accordance with the provisions of Appendix C. 6.3.2.4 Colony count Take the dilution plate, which appears 20 ~ 300 colonies, as the counting criteria (10 ~ 150 colonies for filamentous fungi). Respectively count the number of effective viable bacteria and the number of miscellaneous bacteria. When there is only one dilution AND the average number of colonies is between 20 ~ 300, the average number of colonies is used for calculation. 6.3.3 Determination of the number of fungi Use Martin medium. The determination method is the same as 6.3.2. 6.3.4 Determination of the rate of miscellaneous bacteria Except for the effective bacteria in the sample, all other bacteria are miscellaneous bacteria. The rate of miscellaneous bacteria in the sample is calculated according to formula (3). 6.3.7.3 Particle samples The sample is first crushed and pass through a 1.0 mm test sieve. Make measurement, according to the method of 6.3.7.2. 6.3.8 Determination of the number of fecal coliforms It shall meet the requirements of GB/T 19524.1. 6.3.9 Determination of the mortality of ascaris eggs It shall meet the requirements of GB/T 19524.2. 6.3.10 Determination of cellulase activity and protease activity It shall meet the requirements of Appendix D. 6.3.11 Determination of arsenic, cadmium, lead, chromium, mercury It shall meet the requirements of 5.12 ~ 5.17 in GB 18877-2002. 6.3.12 Inspection of shelf life Before the shelf life indicated in the product manual, measure the corresponding indicators of the product, according to the methods 6.3.1 ~ 6.3.11.7 Inspection rulesThe digital rounding of product technical index in this standard shall comply with the provisions of GB 8170.The qualification judgment of product quality shall comply with the provisions of the comparison method of rounding values in GB 1250-1989. 7.1 Sampling The products, which are processed from each fermenter's bacteria liquid (or each batch of solid fermentation), as a batch, to carry out sampling inspection. During the sampling process, it shall strictly avoid the contamination of bacteria. 7.1.1 Sampling tools Sterile plastic bags (bottles), metal spoons, samplers, measuring cylinders, kraft paper bags, glue, sampling seals, sampling sheets, etc. 7.1.2 Sampling method and quantity Generally, the sampling is carried out in the finished product warehouse, through the random method. 7.2 Inspection classification 7.2.1 Exit-factory inspection 7.2.1.2 The exit-factory inspection items are the items specified in 5.2 and 5.3.1, but do not include the shelf life. 7.2.2 Type inspection 7.3 Judgment rules 7.3.1 Any products, which meet the requirements of one of the following clauses, are qualified products 7.3.2 Any products, which meet the requirements of one of the following clauses, are unqualified products8 Packaging, marking, transportation, storage8.1 Packaging Choose appropriate packaging materials, containers, forms, methods, according to different product dosage forms, to meet the basic requirements of product packaging. 8.2 Markings The content, which are marked on the label, shall comply with the provisions of national laws and regulations. 8.2.1 Product name and trademark It shall indicate the general name of the product, that has been specified by the national standards and industry standards. For the name of the product OR the name of the product with a special purpose, it may be marked, under the general name of the product, by the font of smaller size. 8.2.2 Product specifications It shall indicate the net weight of the product in each package; use the national legal unit of measurement. The error range of the marked net weight shall not exceed ±5% of its stated value. 8.2.3 Implemented standard of product It shall indicate the standard number implemented by the product. 8.2.4 Registration certificate number of product It shall indicate the valid registration certificate number of product. 8.2.5 Name and address of manufacturer It shall indicate the name, address, postal code, contact number of the manufacturer, who has been registered in accordance with the law AND can bear the responsibility for product quality. For the imported products, it may be exempted from the marking of the name and address of the manufacturer; 8.2.6 Date of production or production batch number It shall indicate the production date or production batch number of the product, on the production certificate or product packaging. 8.2.7 Shelf life Use "shelf life _____ months (OR several days, years)" for presentation. 8.3 Transportation There shall be covers, during transportation, to prevent exposure to rain, sun, high temperature. Take appropriate measures, when the temperature is below 0 °C, to ensure product quality. Handle with care, to avoid package damage. It is strictly forbidden to mix and transport it with other items, which are toxic and harmful to the microbial inoculants. 8.4 Storage The products shall be stored in a cool, dry and ventilated warehouse. It shall not be stacked in the open air, to prevent the exposure to sun and rain; avoid the influence of adverse conditions. ......Source: Above contents are excerpted from the full-copy PDF -- translated/reviewed by: www.ChineseStandard.net / Wayne Zheng et al. Tips & Frequently Asked Questions:Question 1: How long will the true-PDF of English version of GB 20287-2006 be delivered?Answer: The full copy PDF of English version of GB 20287-2006 can be downloaded in 9 seconds, and it will also be emailed to you in 9 seconds (double mechanisms to ensure the delivery reliably), with PDF-invoice.Question 2: Can I share the purchased PDF of GB 20287-2006_English with my colleagues?Answer: Yes. The purchased PDF of GB 20287-2006_English will be deemed to be sold to your employer/organization who actually paid for it, including your colleagues and your employer's intranet.Question 3: Does the price include tax/VAT?Answer: Yes. 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