GB 5413.6-2010 PDF English
US$70.00 · In stock · Download in 9 secondsGB 5413.6-2010: National food safety standard -- Determination of insoluble dietary fiber in foods for infants and young children, milk and milk products Delivery: 9 seconds. True-PDF full-copy in English & invoice will be downloaded + auto-delivered via email. See step-by-step procedureStatus: Valid GB 5413.6: Evolution and historical versions
| Standard ID | Contents [version] | USD | STEP2 | [PDF] delivery | Name of Chinese Standard | Status |
| GB 5413.6-2010 | English | 70 |
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National food safety standard -- Determination of insoluble dietary fiber in foods for infants and young children, milk and milk products
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| GB/T 5413.6-1997 | English | 199 |
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Formula foods for infant and young children--Determination of insoluble dietary fiber
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GB 5413.6-2010: National food safety standard -- Determination of insoluble dietary fiber in foods for infants and young children, milk and milk products
---This is an excerpt. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.), auto-downloaded/delivered in 9 seconds, can be purchased online: https://www.ChineseStandard.net/PDF.aspx/GB5413.6-2010
GB
NATIONAL STANDARD OF THE
PEOPLE’S REPUBLIC OF CHINA
National Food Safety Standard - Determination of
Insoluble Dietary Fiber in Foods for Infants and Young
Children, Milk and Milk Products
ISSUED ON: MARCH 26, 2010
IMPLEMENTED ON: JUNE 1, 2010
Issued by: Ministry of Health of the People’s Republic of China
Table of Contents
Foreword ... 3
1 Scope ... 4
2 Normative references ... 4
3 Principle ... 4
4 Reagents and materials ... 4
5 Instruments and apparatuses ... 5
6 Analytical procedures ... 6
7 Expression of analytical result... 7
8 Precision ... 7
National Food Safety Standard - Determination of
Insoluble Dietary Fiber in Foods for Infants and Young
Children, Milk and Milk Products
1 Scope
This Standard stipulates the method for the determination of insoluble dietary fiber in
foods for infants and young children, milk and milk products.
This Standard is applicable to the determination of insoluble dietary fiber in foods for
infants and young children, milk and milk products.
2 Normative references
The references in this Standard are indispensable to the application of this Standard.
In terms of references with a specified date, only versions with a specified date are
applicable to this Standard. In terms of references without a specified date, the latest
version (including all the modifications) is applicable to this Standard.
3 Principle
Use neutral detergent to dissolve and eliminate sugar, starch, protein and pectin in
sample; the residue that cannot be dissolved shall be insoluble dietary fiber, which
mainly includes cellulose, hemicellulose, lignin, keratin and silicon dioxide; it also
includes insoluble ash.
4 Reagents and materials
4.1 Anhydrous sodium sulfite.
4.2 Petroleum ether: boiling range: 30 °C ~ 60 °C.
4.3 Acetone.
4.4 Toluene.
4.5 EDTA disodium salt.
4.6 Sodium tetraborate (contains 10 crystal waters).
m.
d) Reflux condenser.
e) Extraction filtration device: constituted of extraction filtration flask, extraction
filtration pad and water pump.
f) pH meter: division value: 0.01.
6 Analytical procedures
6.1 Weigh-take 0.5 g ~ 1.0 g of solid sample, or, 8.0 g of liquid sample (accurate to 0.1
mg); place it into a tall mouthless beaker. If fat content in the sample exceeds 10%,
such fat needs to be removed first. For example, 1.00 g of sample: use petroleum ether,
at 30 °C ~ 60 °C (4.2) to extract for 3 times; 10 mL each time.
6.2 Add 100 mL of neutral detergent solution (4.13); add 0.5 g of anhydrous sodium
sulfite (4.1).
6.3 Use electric furnace to heat it up. Within 5 min ~ 10 min, make it boil. Then, transfer
it onto the electric heating plate, maintain micro-boiling for 1 h.
6.4 In the acid-resistant glass filter, lay 1 g ~ 3 g of glass wool, then, transfer it into the
oven. At 110 °C, start drying for 4 h. Then, take it out, place it in a dryer; cool it down
to room temperature. Then, weigh it, obtain m1 (accurate to 0.0001 g).
6.5 While the boiled sample is still hot, pour it into the filter; use water pump to start
extraction and filtration. Use 500 mL of hot water (90 °C ~ 100 °C), divide it into several
times to rinse the beaker and the filter, then, extract and filter it, till it reaches dryness.
Rinse the liquid and foam on the lower part of the filter, then, put on a rubber plug.
6.6 Add enzyme solution (4.15) into the filter. The liquid level shall be covered with
fiber. Use fine needle to extrude the bubbles. Add several drops of methylbenzene
(4.4); cover it with a watch glass. At 37 °C, place it in the incubator for an overnight.
6.7 Take out the filter; remove the plug on the bottom; use extraction and filtration to
remove the enzyme solution. In addition, use 300 mL of hot water, divide it into several
times to rinse the residual enzyme solution. Use iodine solution to examine whether
there is any residue of starch. If there is residue of starch, continue to add enzyme for
hydrolysis. If starch is eliminated, drain it, then, use acetone (4.3) to rinse twice.
6.8 Place the filter into the oven. At 110 °C, start drying for 4 h. Then, take it out, place
it into the dryer to cool it down to room temperature. Then, weigh it, obtain m2 (accurate
to 0.0001 g).
......
Source: Above contents are excerpted from the full-copy PDF -- translated/reviewed by: www.ChineseStandard.net / Wayne Zheng et al.
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