GB 5413.5-2010 PDF English
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| Standard ID | Contents [version] | USD | STEP2 | [PDF] delivery | Name of Chinese Standard | Status |
| GB 5413.5-2010 | English | 70 |
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National food safety standard - Determination of lactose and sucrose in foods for infants and young children, milk and milk products
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| GB/T 5413.5-1997 | English | 279 |
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Milk powder and formula foods for infant and young children--Determination of lactose, sucrose and total sugars contents
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GB 5413.5-2010: National food safety standard - Determination of lactose and sucrose in foods for infants and young children, milk and milk products
---This is an excerpt. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.), auto-downloaded/delivered in 9 seconds, can be purchased online: https://www.ChineseStandard.net/PDF.aspx/GB5413.5-2010
GB
NATIONAL STANDARD OF THE
PEOPLE’S REPUBLIC OF CHINA
National food safety standard -
Determination of lactose and sucrose in foods for
infants and young children, milk and milk products
Issued on. MARCH 26, 2010
Implemented on. JUNE 01, 2010
Issued by. Ministry of Health of the People’s Republic of China
Table of Contents
Foreword... 3
1 Scope... 4
2 Normative references... 4
3 Principles... 4
4 Reagents and materials... 4
5 Instruments and apparatuses... 5
6 Analytical procedures... 5
7 Analysis results expression... 6
8 Precision... 7
9 Principles... 7
10 Reagents and materials... 7
11 Instruments and apparatuses... 8
12 Analytical procedures... 9
13 Analysis results expression... 12
14 Precision... 14
15 Other... 14
Foreword
This Standard replaces GB/T 5413.5-1997 “Milk powder and formula foods for
infant and young children - Determination of lactose, sucrose and total sugars
contents”.
As compared with GB/T 5413.5-1997, the main changes of this Standard are
as follows.
- ADD evaporative light-scattering detector.
The previous editions of the standard replaced by this Standard were released
as follows.
- GB 5413-1985, GB/T 5413.5-1997.
National food safety standard -
Determination of lactose and sucrose in foods for
infants and young children, milk and milk products
1 Scope
This Standard specifies the method for determination of lactose and sucrose in
foods for infants and young children, milk and milk products.
This Standard applies to the determination of lactose and sucrose in foods for
infants and young children, milk and milk products.
2 Normative references
The documents referenced in this Standard are indispensable for the
application of this Standard. For the dated references, only the editions with the
dates indicated are applicable to this Standard. For the undated references, the
latest edition (including all the amendments) are applicable to this Standard.
3 Principles
After the lactose and sucrose in the sample are extracted, USE high
performance liquid chromatographic column to separate;
4 Reagents and materials
Unless otherwise specified, the reagents used in this method are analytically
pure; and the water is Grade 1 water specified in GB/T 6682.
4.1 Acetonitrile.
4.2 Acetonitrile. chromatographically pure.
4.3 Standard solutions
4.3.1 Lactose standard stock solution (20 mg/mL). WEIGH 2 g (accurate to 0.1
mg) of the lactose standard sample dried in an oven at 94 °C±2 °C for 2 h;
DISSOLVE in water; USE water to dilute to a 100 mL volumetric flask. PLACE
4.3.4 Sucrose standard working solutions. separately PIPETTE 0 mL, 1 mL, 2
mL, 3 mL, 4 mL, 5 mL of sucrose standard solution (4.3.3) in 10 mL volumetric
flasks; USE acetonitrile (4.1) to dilute to mark. The series of sucrose standard
working solutions are prepared, at concentrations of 0 mg/mL, 1 mg/mL, 2
mg/mL, 3 mg/mL, 4 mg/mL, 5 mg/mL respectively.
5 Instruments and apparatuses
5.1 Balance. The sensitivity is 0.1 mg.
5.3 Ultrasonic oscillator.
6 Analytical procedures
6.1 Sample processing
WEIGH 1 g of solid sample or 2.5 g (accurate to 0.1 mg) of liquid sample into a
50 mL volumetric flask; ADD 15 mL of water at 50 °C~60 °C to dissolve; oscillate
in an ultrasonic oscillator for 10 min;
6.2 Determination
6.2.1 Reference chromatographic conditions
Chromatographic column. Amino column 4.6 mm×250 mm, 5 μm, or column
with equivalent performance;
6.2.2 Making of standard curve
The series of standard working solutions is separately injected into the high
performance liquid chromatograph. The corresponding peak area or peak
height is determined.
7 Analysis results expression
The content of sugar in the sample is calculated according to formula (1).
8 Precision
The absolute difference between the two independent determination results,
obtained under repeated conditions, shall not exceed 5% of the arithmetic mean.
9 Principles
Lactose. After removing the protein from the sample, under the heating
condition, USE the methylene blue as an indicator; the calibrated Fehling’s
solution is directly titrated. According to the volume consumed by the sample
solution, the lactose content is calculated.
10 Reagents and materials
Unless otherwise specified, the reagents used in this method are analytically
pure; and the water is Grade 3 water specified in GB/T 6682.
10.1 Lead acetate.
10.2 Potassium oxalate.
10.3 Disodium hydrogen phosphate.
10.7 Potassium sodium tartrate.
10.8 Sodium hydroxide.
10.9 Phenolphthalein.
10.10 Ethanol.
10.11 Methylene blue.
10.12 Lead acetate solution (200 g/L). WEIGH 200 g of lead acetate;
DISSOLVE in water and dilute to 1000 mL.
10.13 Potassium oxalate-disodium hydrogen phosphate solution. WEIGH 30 g
of potassium oxalate, 70 g of disodium hydrogen phosphate; DISSOLVE in
water and dilute to 1000 mL.
10.14 Hydrochloric acid (1+1). 1 volume of hydrochloric acid is mixed with 1
volume of water.
10.17 Phenolphthalein solution (5 g/L). WEIGH 0.5 g of phenolphthalein in 100
mL of ethanol with a volume fraction of 95%.
10.18 Methylene blue solution (10 g/L). WEIGH 1 g of methylene blue in 100
mL of water.
11 Instruments and apparatuses
11.1 Balance. The sensitivity is 0.1 mg.
12 Analytical procedures
12.1 Calibration of Fehling’s solution
12.1.1 USE lactose to calibrate
12.1.1.1 WEIGH about 0.75 g (accurate to 0.1 mg) of the lactose standard
sample pre-dried in an oven at 94 °C±2 °C for 2 h; USE water to dissolve and
dilute to 250 mL. Inject the lactose solution into a 50 mL burette for titration.
12.2 Determination of lactose
12.2.1 Sample processing
12.2.1.1 WEIGH 2 g of infant food or skim powder, 2.5 g of whole sugared
powder or whole powder, 1 g of whey powder, accurate to 0.1 mg. USE 100 mL
of water to dissolve several times and WASH into a 250 mL volumetric flask.
12.2.2 Titration
12.2.2.1 Pre-titration. The operation is the same as 12.1.1.2.
12.2.2.2 Precise titration. The operation is the same as 12.1.1.3.
12.3 Determination of sucrose
13 Analysis results expression
13.1 Lactose
The content X of lactose in the sample shall be calculated according to formula
(6)
13.3 If the ratio of sucrose to lactose in the sample exceeds 3.1, when
calculating the lactose, the corrected value in Table 2 shall be added to the
titration volume and then Table 1 is referred.
14 Precision
The absolute difference between the two independent determination results,
obtained under repeated conditions, shall not exceed 1.5% of the arithmetic
mean.
15 Other
The detection limit of Method 1 of this Standard is 0.3 g/100 g. The detection
limit of Method 2 is 0.4 g/100 g.
......
Source: Above contents are excerpted from the full-copy PDF -- translated/reviewed by: www.ChineseStandard.net / Wayne Zheng et al.
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