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GB/T 4789.36-2008 (GBT 4789.36-2008)

GB/T 4789.36-2008_English: PDF (GBT4789.36-2008)
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GB/T 4789.36-2008English619 Add to Cart Days<=3 Microbiological examination of food hygiene -- Examination of Escherichia coli O157: H7/NM GB/T 4789.36-2008 Obsolete GB/T 4789.36-2008

BASIC DATA
Standard ID GB/T 4789.36-2008 (GB/T4789.36-2008)
Description (Translated English) Microbiological examination of food hygiene. Examination of Escherichia coli O157: H7/NM
Sector / Industry National Standard (Recommended)
Classification of Chinese Standard C53
Classification of International Standard 07.100.30
Word Count Estimation 16,162
Date of Issue 2008-05-16
Date of Implementation 2008-11-01
Adopted Standard FDA Chapter 4-2002, MOD; NMKL No.164-1999, MOD; AOAC-RI VIDAS E.Coli O0157 Test No.010502, MOD, etc.
Drafting Organization China Disease Prevention and Control Center of Nutrition and Food Safety
Administrative Organization Ministry of Health
Regulation (derived from) National Standard Approval Announcement 2008 No.9 (Total No.122)
Proposing organization People's Republic of China Ministry of Health
Issuing agency(ies) Ministry of Health of People's Republic of China; Standardization Administration of China
Summary This standard specifies the food in Escherichia coli O157: H7 and O157: NM test methods. This standard applies to Escherichia coli in food and food poisoning samples O157

GB/T 4789.36-2008
Microbiological examination of food hygiene Examination of Escherichia coli O157. H7/NM
ICS 07.100.30
C53
National Standards of People's Republic of China
Microbiological examination of food hygiene
Escherichia coli O157. H7/NM inspection
Posted 2008-05-16
2008-11-01 implementation
People's Republic of China Ministry of Health
Standardization Administration of China released
Foreword
The standards are divided into four methods, namely.
--- First Law. General culture;
--- The second method. MACS capture method;
--- Third method. automated fluorescent enzyme-linked immunoassay analyzer screening method;
--- Fourth method. automated pathogen detection screening system.
The standard first law revision adopted by the US Food and Drug Administration (FDA) "Bacterial Analysis Handbook" CAP 4A (2002) (Bac-
teriologicalAnalyticalManual, Chapter4A, 2002); The second method uses a modification of the Nordic Committee on Food Analysis (NMKL)
No. 164,1999; third law revision adopts the International Institute analyst (AOACINTERNATIONAL) AOAC-RIPerform-
The standard method first main difference compared with the US FDA/BAM as follows.
--- Enrichment broth mEC + n medium to replace the EEB broth.
--- Increased CHROMagarO157 chromogenic medium.
The second standard method and NMKL, No. The main difference compared to 164,1999 method as follows.
--- Enrichment medium to mEC + n broth replaced mTSB broth.
--- Modify enrichment culture temperature of 41.5 ℃ ± 0.5 ℃ to 36 ℃ ± 1 ℃.
--- Increased CHROMagarO157 chromogenic medium.
The main difference compared to 010,502 method of enrichment medium to mEC + n broth replaced mTSB broth.
Compared to major changes other than raw food enrichment broth mEC + n medium to replace the EEB broth.
Appendix A of this standard is a normative appendix.
This standard is proposed and administered by the People's Republic of China Ministry of Health.
This standard was drafted. Chinese Center for Disease Control Nutrition and Food Safety.
Units involved in the drafting of this standard. Disease Prevention and Control Center of Henan Province, the Beijing Municipal Center for Disease Control and Prevention, Disease Control and Prevention in Zhejiang Province
Centre, Jilin Province Disease Prevention and Control Center, Disease Prevention and Control Center in Hubei Province, People's Republic of China Shanghai Entry-Exit Inspection and Quarantine Bureau, in
State Inspection and Quarantine Science Research Institute.
The main drafters of this standard. Xiumei, Liu Xing Guang, Zhang Xiuli, Chen Qian, Chengsu Yun, Liu Guihua, Xiemao Hui, Li Xiaohong, Tian Jing.
Microbiological examination of food hygiene
Escherichia coli O157. H7/NM inspection
1 Scope
This standard specifies the foods Escherichia coli O157. H7 and O157. NM testing methods.
This standard applies to Escherichia coli food poisoning and food samples O157. H7 and O157. NM test.
2 Equipment and Materials
In addition to the microbiological laboratory conventional sterilization and cultivation equipment, other equipment and materials as follows.
2.1 Balance. a sense of the amount of 0.1g, 0.01g.
2.2 homogenizer.
2.3 Refrigerator. 2 ℃ ~ 5 ℃.
2.4 incubator. 36 ℃ ± 1 ℃.
2.5 constant temperature water bath. 46 ℃ ± 0.5 ℃, 100 ℃.
2.6 Biological Microscope. 10 × ~ 100 ×.
2.7 bacterial concentration turbidity tube. No. MacFarland0.5 or turbidimeter.
2.8 sterile conical flask. 500mL, 250mL.
2.9 sterile petri dish. diameter of 90mm.
2.10 sterile tube. 10mm × 75mm.
2.11 sterile pipette. 1mL (with 0.01mL scale), 10.0mL (with 0.1mL scale) or micro pipettes and tips.
2.12 pH meter or pH colorimetric tubes or precision pH test paper.
2.13 long-wave UV light. 366nm, power ≤6W.
2.14 magnetic plate, magnetic plate rack, DynalMX1 sample mixer 1).
2.15 automated microbial identification system (VITEK) 2).
2.16 ELFA automatic immunoassay analyzer (miniVIDAS or VIDAS) 2).
2.17 automated pathogen detection system (BAX system) 3).
1) Product name provided by the Norwegian company Dynal products. This information is given for the convenience of use