GB/T 20944.3-2008 PDF English
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| GB/T 20944.3-2008 | English | 125 |
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Textiles - Evaluation for antibacterial activity - Part 3: Shake flask method
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GB/T 20944.3-2008: Textiles - Evaluation for antibacterial activity - Part 3: Shake flask method---This is an excerpt. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.), auto-downloaded/delivered in 9 seconds, can be purchased online: https://www.ChineseStandard.net/PDF.aspx/GBT20944.3-2008
GB
NATIONAL STANDARD OF THE
PEOPLE’S REPUBLIC OF CHINA
ICS 59.080.01
W 04
Textiles - Evaluation for antibacterial activity - Part 3.
Shake flask method
Issued on. APRIL 29, 2008
Implemented on. DECEMBER 01, 2008
Issued by. General Administration of Quality Supervision, Inspection and
Quarantine;
Standardization Administration of the People's Republic of
China.
Table of Contents
Foreword... 3
1 Scope... 4
2 Normative references... 4
3 Terms and definitions... 4
4 Safety precautions... 5
5 Principle... 5
6 Test equipment... 5
7 Medium and reagents... 6
8 Test strain... 6
9 Preparation of test bacteria... 7
10 Preparation of sample... 9
11 Test operation... 10
12 Calculation and evaluation of results... 12
13 Test report... 13
Foreword
GB/T 20944 “Textiles - Evaluation for antibacterial activity” consists of three
parts.
- Part 1.Agar diffusion plate method;
- Part 2.Absorption method;
- Part 3.Shake flask method.
This Part is Part 3 of GB/T 20944.
This Standard was proposed by China Textile Industry Association.
This Part shall be under the jurisdiction of Subcommittee on Basics of National
Technical Committee on Textiles of Standardization Administration of China
(SAC/TC 209/SC 1).
Main drafting organizations of this Part. Shenzhen Beiyue Haiwei Chemical Co.,
Ltd., National Textile Products Quality Supervision and Inspection Center,
South Test Center of Shanghai Academy of Textile Science, Chinese People's
Liberation Army Special Protective Clothing Quality Inspection Center,
Hangzhou Xinhua Nufte Sanitary Material Co., Ltd., Zhejiang Xiaoxue Clothing
Co., Ltd., Zhejiang Langsha Hosiery Co., Ltd., China Knitting Industry
Association.
Main drafters of this Part. Zou Haiqing, Wang Junqi, Wang Youbin, Liu Chen,
Zhang Hua, Ni Jiyun, Li Hong, Sheng Junqi, Liu Ailian, Wang Zhi.
This Part is issued for the first time.
Textiles - Evaluation for antibacterial activity - Part 3.
Shake flask method
1 Scope
This Part of GB/T 20944 specifies quantitative test and evaluation method for
determining the antibacterial properties of textiles by the shake flask method.
This Part is applicable to various textile products such as down, fiber, yarn,
fabric, and products with special shapes, especially applicable to non-
dissolvable antibacterial textile products.
This Part does not involve the evaluation of the safety of antibacterial products.
2 Normative references
The provisions in following documents become the provisions of this Part of
GB/T 20944 through reference in this Standard. For dated references, the
subsequent amendments (excluding corrigendum) or revisions do not apply to
this Standard, however, parties who reach an agreement based on this
Standard are encouraged to study if the latest versions of these documents are
applicable. For undated references, the latest edition of the referenced
document applies.
GB/T 12490, Textiles - Tests for color fastness - Color fastness to domestic
and commercial laundering (GB/T 12490-2007, ISO 105-C06.1994, MOD)
3 Terms and definitions
For the purposes of this document, the following terms and definitions apply.
3.1 antibacterial activity
The activity of textiles to inhibit the growth and reproduction of bacteria on the
fabric.
4 Safety precautions
Safety instructions. The test bacteria used in this test can cause human
infection and disease.
5 Principle
Put the sample and the control fabric into an Erlenmeyer flask that is filled with
a certain concentration of test bacteria liquid.
6 Test equipment
6.1 Spectrophotometer. The detection wavelength is 475nm or 660nm, suitable
for testing the concentration of test bacteria.
6.2 Constant temperature incubator. temperature control accuracy is ±1°C.
6.3 Water bath. The temperature can be maintained at 46°C±2°C.
6.7 Autoclave. The temperature can be kept at 121°C and the pressure can be
kept at 103kPa.
6.8 Erlenmeyer flask with stopper. The capacity is 250mL.
6.9 Petri dish. 90mm in diameter.
7 Medium and reagents
The reagents used in the test shall be analytically pure or suitable for
microbiological tests. The test water shall be pure water, such as distilled water.
7.1 Nutrient broth
7.2 Nutrient agar medium
7.4 0.03mol/L PBS (phosphate) buffer
Disodium phosphate 2.84g
8 Test strain
8.1 Strain
Gram-positive bacteria. Staphylococcus aureus (ATCC 6538);
Gram-negative bacteria. Escherichia coli (8099 or one of ATCC 11229, ATCC
8739, ATCC 29522), or Klebsiella pneumoniae (ATCC 4352). Choose one
according to the needs;
8.2 Strain transformation and preservation
After activation of the freeze-dried bacteria, inoculate the inclined tube culture.
Then store in the refrigerator (5°C~10°C) as preservation bacteria. Planting
shall be changed every month. Store in refrigerator (5°C~10°C) after planting.
8.3 Marks
The following information shall be marked for each strain.
9 Preparation of test bacteria
9.1 Cultivation and preparation of bacterial liquid
9.1.1 Two-step pre-culture procedure to prepare bacterial inoculum
suspension
9.1.2 Preparation of bacterial inoculum
Use a pipette to draw 2mL~3mL from the bacterial suspension (9.1.1)
(reference value. This step adjusts the number of live bacteria inoculated, the
lower limit for Escherichia coli, and the upper limit for Staphylococcus aureus).
9.2 Cultivation and preparation of Candida albicans broth
9.2.1 Preparation of Candida albicans inoculum suspension by two-step
pre-culture procedure
Use a pipette to draw 2mL~4mL from the Candida albicans suspension (9.2.1).
Transfer to a test tube containing 9mL of 0.03mol/L PBS buffer (7.4). Perform a
10-fold serial dilution operation. Mix well.
10 Preparation of sample
10.1 Sample washing
If the sample needs to be washed, one of the 10.1.1 or 10.1.2 washing methods
shall be used for operation. Indicate the washing method used in the test report.
10.2 Sample
Cut the antibacterial fabric sample and the control fabric into pieces of about
5mm×5mm in size. Weigh 0.75g±0.05g as a sample. Wrap it in small pieces of
paper.
10.3 Sample sterilization
Put the small paper bag containing the sample into the autoclave. Sterilize at
121°C, 103kPa for 15min for later use.
11 Test operation
11.1 Sample and reagent bottling
Prepare 9 250ml Erlenmeyer flasks. Respectively add 0.75g±0.05g of control
fabric to each of the 3 flasks, and 0.75g±0.05g of antibacterial fabric sample to
each of 3 flasks. The other 3 flasks are blank without sample. Then add
70mL±0.1mL of 0.03mol/L PBS buffer (7.4) to each flask.
11.3 "0" contact time sampling
Use a pipette to draw 1mL ± 0.1mL of solution from each of the 6 flasks
prepared with a contact time of "0". Transfer to a test tube containing 9mL ±
0.1mL of 0.03 mol/L PBS buffer (7.4). Mix well. Use 10 times dilution method to
make another dilution. Mix well. Pipette 1mL±0.1mL into a sterilized petri dish,
pour about 15mL of nutrient agar medium (7.2) or Sabouraud agar medium
(7.3).
11.5 Dilution culture and determination of colony number
After the specified time, draw 1mL±0.1mL of test solution from each flask.
Transfer to a test tube containing 9mL±0.1mL of 0.03mol/L PBS buffer (7.4).
Mix well. Use 10-fold dilution method to serially dilute to the appropriate dilution
multiple.
......
Source: Above contents are excerpted from the full-copy PDF -- translated/reviewed by: www.ChineseStandard.net / Wayne Zheng et al.
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