GB/T 18932.22-2003 PDF English
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Method for the determination of fructose, glucose, sucrose, maltose contents in honey -- Liquid chromatography refractive index detection method
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GB/T 18932.22-2003: Method for the determination of fructose, glucose, sucrose, maltose contents in honey -- Liquid chromatography refractive index detection method
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GB
ICS 67.180.10
X 31
NATIONAL STANDARD
OF THE PEOPLE’S REPUBLIC OF CHINA
Method for the determination of fructose, glucose,
sucrose, maltose contents in honey - Liquid
chromatography refractive index detection method
Issued on: DECEMBER 26, 2003
Implemented on: JUNE 1, 2004
Issued by. General Administration of Quality Supervision, Inspection
and Quarantine of the People’s Republic of China
Table of Contents
Foreword ... 3
1 Scope ... 4
2 Normative references ... 4
3 Principles ... 4
4 Reagents & materials ... 5
5 Instruments ... 5
6 Sample preparation and storage ... 6
7 Determination steps ... 6
8 Calculation of results ... 7
9 Precision ... 8
Foreword
This Part of GB/T 18932 equivalently adopts the Canadian Standard ACC-017-
V2.0 “Method for the determination of carbohydrate - Liquid chromatography
method”. It only modifies some minor contents.
Appendix A of this Part is informative.
This Part was proposed by Qinhuangdao Entry - Exit Inspection and Quarantine
Bureau of the People's Republic of China.
This Part shall be under the jurisdiction of All China Federation of Supply and
Marketing Cooperatives.
The main drafting organization of this Part. Qinhuangdao Entry - Exit Inspection
and Quarantine Bureau of the People's Republic of China.
The drafting organization of this Part. National Quality Supervision and
Inspection Center for Bee Products.
Main drafters of this Part. Pang Guofang, Cao Yanzhong, Li Zijian, Jia
Guangqun, Fan Chunlin, Zhang Jinjie, Li Xuemin and Shi Yuqiu.
This Part was issued as a national standard for the first time.
Method for the determination of fructose, glucose,
sucrose, maltose contents in honey - Liquid
chromatography refractive index detection method
1 Scope
This Part of GB/T 18932 specifies the method for the determination of fructose,
glucose, sucrose, maltose contents in honey.
This Part is applicable to the determination of fructose, glucose, sucrose,
maltose contents in honey.
The method detection limit of this Part. fructose, glucose, and sucrose are 0.5%;
maltose is 0.2%.
2 Normative references
The following standards contain the provisions which, through reference in this
Part of the GB/T18932, constitute the provisions of this Part. For the dated
references, all subsequent amendments (excluding corrections) or revisions do
not apply to this Part. However, the parties who enter into agreement based on
this Part are encouraged to investigate whether the latest versions of these
standards are applicable. For undated reference documents, the latest versions
apply to this Part.
GB/T 6379 Precision of test methods - Determination of repeatability and
reproducibility for a standard test method by inter-laboratory tests (GB/T
6379-1986, neq ISO 5725.1981)
GB/T 6682 Water for analytical laboratory use - Specification and test
methods (GB/T 6682-1992, neq ISO 3696.1987)
3 Principles
Use water to dissolve the sample. After acetonitrile volume is constant, filter it
with 0.45 μm filterable membrane. Use the liquid chromatography refractive
index detector to determine. And adopt the external standard method to quantify.
5.2 Analytical balance. two balances of which one’s sensitivity is 0.1 mg, and
the other’s sensitivity is 0.01 g.
5.3 Injector. 10 mL.
5.4 Organic phase filtration membrane. 0.45 μm.
5.5 Sample flask. 1.5 mL.
5.6 Volumetric flasks. 10mL, 25 mL, 50 mL, 100 mL.
6 Sample preparation and storage
6.1 Sample preparation
For non-crystalized laboratory sample, stir it evenly. For crystalized sample, in
a closed circumstance, place the crystalized sample in the water bath at no
more than 60°C; warm it; oscillate it till it is totally melted; stir it and cool it to
room temperature. Take 0.5 kg as the sample. Place the prepared sample in
the sample flask, seal and mark it.
6.2 Sample storage
Store the sample at room temperature.
7 Determination steps
7.1 Extraction
Weigh 5 g of sample, accurate to 0.001 g. Place it into a 50 mL beaker; add 30
mL of water. Use a glass rod to stir and make the sample completely dissolved.
Transfer it to a 100 mL flask. Use 10 mL of water to wash the beaker for three
times. Then transfer it to the aforementioned 100 mL flask. Use acetonitrile to
make the constant volume; mix it evenly. Use 0.45 μm filter membrane to filter
the sample solution into the sample flask for liquid chromatography.
7.2 Determination
7.2.1 Liquid chromatography conditions
a) Chromatographic column. carbohydrate analysis column 10μm,
300mm×3.9 mm (i.d);
b) Mobile phase. acetonitrile + water (77+23);
c) Flow rate. 1.0 mL/min;
Where,
X - component content in the determined sample, in grams per 100 grams
(g/100 g);
c - solution concentration of determined components, obtained from the
standard work curve, in grams per 100 milliliters (g/100 mL);
V - constant volume of sample solution, in milliliters (mL);
m - mass of weighed sample, in grams (g).
Note. The blank value shall be deducted from the result.
9 Precision
The precision data in this Part is determined in accordance with the provisions
of GB/T 6379. The repeatability and reproducibility values are calculated in 95%
confidence.
9.1 Repeatability
Under conditions of repeatability, the absolute difference between two
independent test results obtained shall not exceed the repeatability limit (r). The
content range of fructose, glucose, sucrose and maltose in honey and
repeatability equation are shown in Table 2.
Table 2 The Content Range, Repeatability Equation and Reproducibility
Equation
Name Content Range (g/100g) Repeatability Limit (r) Reproducibility Limit (R)
Fructose 21.31~39.62 r=–0.0095 m + 0.5420 lgR=0.4529 lgm – 0.5978
Glucose 20.54~36.15 r=–0.0094 m + 0.4127 lgR=0.0905 lgm – 0.4236
Sucrose 0.22~5.95 r=–0.0115 m + 0.1579 lgR=0.0011 m + 0.2149
Maltose 0.98~6.99 r=–0.0047 m + 0.1623 lgR=0.1474 lgm – 0.8122
If the difference exceeds the repeatability limit (r), then the test result shall be
discarded, and recomplete the determinations of two independent tests.
9.2 Reproducibility
Under conditions of reproducibility, the absolute difference between two
independent test results obtained shall not exceed the reproducibility limit (R).
The content range of fructose, glucose, sucrose and maltose in honey and
reproducibility equation are shown in Table 2.
......
Source: Above contents are excerpted from the full-copy PDF -- translated/reviewed by: www.ChineseStandard.net / Wayne Zheng et al.
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