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GB 31604.36-2016 PDF English

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GB 31604.36-2016: National food safety standard - Food contact materials and products - Determination of creosote in cork
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GB 31604.36-2016: National food safety standard - Food contact materials and products - Determination of creosote in cork


---This is an excerpt. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.), auto-downloaded/delivered in 9 seconds, can be purchased online: https://www.ChineseStandard.net/PDF.aspx/GB31604.36-2016
GB NATIONAL STANDARD OF THE PEOPLE’S REPUBLIC OF CHINA National Food Safety Standard - Food contact materials and products - Determination of creosote in cork Issued on: OCTOBER 19, 2016 Implemented on: APRIL 19, 2017 Issued by. National Health and Family Planning Commission of People's Republic of China

Table of Contents

Foreword . 3 1 Scope .. 4 2 Principle.. 4 3 Reagents and materials .. 4 4 Instruments and equipment . 5 5 Analytical steps . 5 6 Expression of the analytic result .. 6 7 Precision. 7 8 Others . 7 Appendix A Names, CAS number, chemical structure formula and molecular formula of 9 kinds of creosote compounds .. 8 Appendix B Examples for determination of 9 kinds of creosote compounds .. 10 Foreword This standard replaces SN/T 2828-2011 "Food contact materials for export. Wood materials. Determination of cresol in cork. Gas chromatography-mass spectrometry method". Compared with SN/T 2828-2011, the main differences of this standard are as follows. - Changed the name of the standard to "National Food Safety Standard - Food contact materials and products -Determination of creosote in cork"; - Replaced single point method to standard curve method for quantitative method. National Food Safety Standard - Food contact materials and products - Determination of creosote in cork

1 Scope

This standard specifies the gas chromatography-mass spectrometry method for the determination of creosote in cork for food contact materials. This standard is applicable to the determination of creosote in cork for food contact materials. 2 Principle Extract the creosote in the sample by Soxhlet extractor with absolute ethyl alcohol, concentrate the extracted solution and the meter volume. Filter the extracted solution by 0.45μm organic microporous membrane, then determine by gas chromatography-mass spectrometry (GC-MS). Use retention time for qualitative determination and external standard method for quantitative determination.

3 Reagents and materials

Unless otherwise stated, the reagents used in this method all shall be analytical pure. The water shall be the primary water specified in GB/T 6682. 3.1 Reagents Absolute ethyl alcohol (CH3CH2OH). 3.2 Standard substance Standard creosote. purity no less than 98%, see appendix A. 3.3 Preparation of standard solutions 3.3.1 Preparation of standard reserve solution Prepare the standard reserve solution 100 μg/mL with absolute ethyl alcohol. 3.3.2 Preparation of standard working solution Dilute the standard reserve solution into 0.01μg/mL, 0.02μg/mL, 0.05μg/mL, 0.1μg/mL and 0.2μg/mL of standard working solution with absolute ethyl alcohol. Or make standard working solution with suitable concentration as required. Note. The valid period for standard solution stored in 0~4°C refrigerator is 6 months.

4 Instruments and equipment

4.1 Gas chromatography-mass spectrometry. 4.2 Soxhlet extractor. 150mL. 4.3 Rotary evaporator. 4.4 Cutting grinder. 4.5 Organic microporous membrane. pore diameter 0.45μm.

5 Analytical steps

5.1 Sample preparation Take appropriate amount of cork sample, cut and ground into powder with diameter less than 1mm by cutting and grinding machine, fully mix and store in a closed container avoiding light. 5.2 Sample extraction Weigh accurately 2g of sample (accuracy to 1mg), put in the cylinder of 150mL Soxhlet extractor and add 80mL of absolute ethyl alcohol into round-bottom flask. Exact at 80°C and 95°C for 4 hours with the reflux no less than 4 times in 1 hour. After cooling, evaporate with rotating evaporator at 50°C until about 5 mL is left. Then transferred and meter to 10 mL with absolute ethyl alcohol. Filter by organic filter membrane and wait for determination by gas chromatography-mass spectrometry (GC-MS). 5.3 Determination 5.3.1 Instrument reference condition 5.3.1.1 Gas chromatography-mass spectrometry reference condition The following parameters are available for reference, see Appendix B for example. ......
Source: Above contents are excerpted from the full-copy PDF -- translated/reviewed by: www.ChineseStandard.net / Wayne Zheng et al.


      

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