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SN/T 4818-2017 English PDF

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SN/T 4818-2017: Determination of ractopamine, salbutamol, clenbuterol hydrochloride in edible animal for imported and export. Enzyme linked immunosorbent assay method
Status: Valid
Standard IDUSDBUY PDFLead-DaysStandard Title (Description)Status
SN/T 4818-2017169 Add to Cart 3 days Determination of ractopamine, salbutamol, clenbuterol hydrochloride in edible animal for imported and export. Enzyme linked immunosorbent assay method Valid

Similar standards

GB/T 9959.1   SB/T 10381   SB/T 10294   SN/T 4811   SN/T 4812   SN/T 4810   

Basic data

Standard ID: SN/T 4818-2017 (SN/T4818-2017)
Description (Translated English): Determination of ractopamine, salbutamol, clenbuterol hydrochloride in edible animal for imported and export. Enzyme linked immunosorbent assay method
Sector / Industry: Commodity Inspection Standard (Recommended)
Classification of Chinese Standard: X22
Word Count Estimation: 7,764
Date of Issue: 2017-07-21
Date of Implementation: 2018-03-01
Regulation (derived from): National Quality Inspection (2017) No. 337
Issuing agency(ies): General Administration of Customs

SN/T 4818-2017: Determination of ractopamine, salbutamol, clenbuterol hydrochloride in edible animal for imported and export. Enzyme linked immunosorbent assay method


---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
(Determination of lycopamine, salbutamol and clenbuterol hydrochloride in edible animals by enzyme-linked immunosorbent assay) People's Republic of China entry and exit inspection and quarantine industry standards Import and export of food animals in ractopamine, Determination of salbutamol and clenbuterol hydrochloride Enzyme linked immunosorbent assay Determinationofractopamine, salbutamol, clenbuterolhydrochlorideinedible Released on.2017-07-21 2018-03-01 implementation People's Republic The General Administration of Quality Supervision, Inspection and Quarantine issued

Foreword

This standard was drafted in accordance with the rules given in GB/T 1.1-2009. Please note that some of the contents of this document may involve patents. The issuing organization of this document is not responsible for identifying these patents. This standard is proposed and managed by the National Certification and Accreditation Administration. This standard was drafted. Shandong Entry-Exit Inspection and Quarantine Bureau of the People's Republic of China. The main drafters of this standard. Sun Mingjun, Sun Tao, Liang Guanghui, Liang Chengzhu, Zheng Xiaolong, Yue Zhiqin, Zhu Laihua, Xu Wei. Import and export of food animals in ractopamine, Determination of salbutamol and clenbuterol hydrochloride Enzyme linked immunosorbent assay

1 Scope

This standard specifies the enzymes for the detection of ractopamine, salbutamol and clenbuterol residues in the plasma and urine of imported and exported food animals. Combined immunosorbent assay. This standard applies to the blood and urine of imported and exported poultry, ractopamine, salbutamol and clenbuterol hydrochloride Rapid screening test, suitable for entry and exit inspection and quarantine.

2 Normative references

The following documents are indispensable for the application of this document. For dated references, only the dated version applies to this article. Pieces. For undated references, the latest edition (including all amendments) applies to this document. GB/T 6682 Analytical laboratory water specifications and test methods

3 Principle

Using horseradish peroxidase (HRP) and chemical methods to utilize the specific binding properties of antigen-antibodies and the efficient catalytic action of enzymes Clenbuterol (CL) is coupled to form a horseradish peroxidase-labeled clenbuterol. The coated antibody on the solid phase carrier and the specific anti-gram The Lentrol antibody binds to immobilize the anti-Clenbuter antibody, and then adds the Clenbuterol and horseradish peroxidase-labeled Clenbuterol to be tested. They are competitively bound to the Clenbuterol antibody, washed with a color developing agent, and the amount of clenbuterol to be measured is measured based on the color change of the color developing agent. If When the Clenbuterol is to be tested, the enzyme labeled with Clenbuter is less, the color of the developer is light, and vice versa. Visually or colorimetrically The Clenbuterol content in the product, the optimal wavelength for colorimetry is 450 nm. The principle of salbutamol and ractopamine is the same as that of Clenbuterol.

4 reagents and materials

4.1 1mol/L sodium hydroxide. See Appendix A for preparation. The test water should meet the requirements of GB/T 6682. 4.2 Ethyl acetate. analytically pure. 4.3 β-glucuronidase. β-glucuronidase 111000 U/mL. 4.4 Potassium phosphate buffer. 4.5 Borate buffer. 4.6 coating buffer. 4.7 Wash buffer. 4.8 Blocking buffer. 4.9 Dilution buffer. 4.10 Horseradish peroxidase labeled conjugate dilution.
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