SN/T 4750-2017 English PDF

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SN/T 4750-2017: Quarantine protocol for swine torque teno sus virus
Status: Valid

Standard ID	USD	BUY PDF	Lead-Days	Standard Title (Description)	Status
SN/T 4750-2017	279	Add to Cart	3 days	Quarantine protocol for swine torque teno sus virus	Valid

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Basic data

Standard ID: SN/T 4750-2017 (SN/T4750-2017)
Description (Translated English): Quarantine protocol for swine torque teno sus virus
Sector / Industry: Commodity Inspection Standard (Recommended)
Classification of Chinese Standard: B41
Word Count Estimation: 12,192
Date of Issue: 2017-05-12
Date of Implementation: 2017-12-01
Regulation (derived from): State-Quality-Inspection-Accreditation (2017) 228
Issuing agency(ies): General Administration of Customs

SN/T 4750-2017: Quarantine protocol for swine torque teno sus virus

---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
(Technical specification for porcine fine loop virus quarantine) People's Republic of China entry and exit inspection and quarantine industry standards Swine fine ring virus quarantine technical specification Released on.2017-05-12 2017-12-01 implementation People's Republic The General Administration of Quality Supervision, Inspection and Quarantine issued

Foreword

This standard was drafted in accordance with the rules given in GB/T 1.1-2009. This standard is proposed and managed by the National Certification and Accreditation Administration. This standard was drafted. Jiangxi Entry-Exit Inspection and Quarantine Bureau of the People's Republic of China, Shenzhen Institute of Inspection and Quarantine, Jiangxi Province Inspection Institute of Epidemiology, Shenzhen Entry-Exit Inspection and Quarantine Bureau of the People's Republic of China, Shanghai Entry-Exit Inspection and Quarantine Bureau of the People's Republic The main drafters of this standard. Yang Chunhua, Sun Siyang, Zhang Qiang, Chen Qingfu, Zhou Yan, Sun Jie, Zhu Jianxin, Luo Qiuhong, Hua Qunyi. Swine fine ring virus quarantine technical specification

1 Scope

This standard specifies the method for the detection of porcine loop virus and the real-time fluorescent PCR assay. This standard applies to the detection of porcine circovirus nucleic acid in pigs and their products.

2 Normative references

The following documents are indispensable for the application of this document. For dated references, only the dated version applies to this article. Pieces. For undated references, the latest edition (including all amendments) applies to this document. GB/T 6682 Analytical laboratory water specifications and test methods GB 19489 General requirements for laboratory biosafety SN/T 2025 Animal Quarantine Laboratory Biosafety Practices

3 pollution prevention measures

All operations covered by this standard shall comply with the relevant provisions of GB 19489 and SN/T 2025.

4 Abbreviations

The following abbreviations apply to this document. 4.1 DEPC. diethylpyrocarbonate (diethypyrocarbonate) 4.2 DNA. deoxyribonucleic acid 4.3 EDTA. ethylenediaminetetraacetic acid 4.4 PCR. polymerase chain reaction (polymerase chain reaction) 4.5 RNA. ribonucleic acid 4.6 SDS. sodium dodecyl sulfate (sodiumdodecylsulfate) 4.7 TTSuV. SwineTorqueTenoSusVirus (see Appendix A) 5 Equipment, materials and reagents 5.1 Equipment, materials PCR instrument, real-time fluorescence PCR instrument, electrophoresis instrument, gel imaging system, autoclave, refrigerator, micro-adjustable pipette and tip, cold Freeze centrifuge, 1.5 mL centrifuge tube. 5.2 Reagents Unless otherwise stated, the reagents used in this standard are of molecular biology grade, and the test water should meet the requirements of GB/T 6682. sample cracking Buffer (see B.4), 10% SDS, 20 g/L proteinase K, RNase, phenol/chloroform/isoamyl alcohol (25.24.1), isopropanol, 70% Ethanol (prepared with absolute ethanol and DEPC water), DEPC water, PCR reaction premix, PCR primers (see B.6), running buffer, agar ......

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