SN/T 4748-2017 English PDF

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SN/T 4748-2017: Quarantine protocol for lymphocytic choriomeningitis
Status: Valid

Standard ID	USD	BUY PDF	Lead-Days	Standard Title (Description)	Status
SN/T 4748-2017	259	Add to Cart	3 days	Quarantine protocol for lymphocytic choriomeningitis	Valid

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Basic data

Standard ID: SN/T 4748-2017 (SN/T4748-2017)
Description (Translated English): Quarantine protocol for lymphocytic choriomeningitis
Sector / Industry: Commodity Inspection Standard (Recommended)
Classification of Chinese Standard: C62
Word Count Estimation: 11,144
Date of Issue: 2017-05-12
Date of Implementation: 2017-12-01
Regulation (derived from): State-Quality-Inspection-Accreditation (2017) 228
Issuing agency(ies): General Administration of Customs

SN/T 4748-2017: Quarantine protocol for lymphocytic choriomeningitis

---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
(Technical specification for quarantine of lymphocytic choriomeningitis) People's Republic of China entry and exit inspection and quarantine industry standards Lymphocytic choriomeningitis Quarantine technical specification Released on.2017-05-12 2017-12-01 implementation People's Republic The General Administration of Quality Supervision, Inspection and Quarantine issued

Foreword

This standard was drafted in accordance with the rules given in GB/T 1.1-2009. This standard is proposed and managed by the National Certification and Accreditation Administration. This standard was drafted. Shanghai Entry-Exit Inspection and Quarantine Bureau of the People's Republic of China, Jiangxi Entry-Exit Inspection and Quarantine Bureau, Guangdong Entry-Exit Inspection and Quarantine Bureau of the People's Republic of China. The main drafters of this standard. Zhang Qiang, Wang Yan, Yang Chunhua, Xiong Wei, Lin Yingxi, Li Shuqing, Wang Qiaoquan, Zhu Shikang, Li Jian. Lymphocytic choriomeningitis Quarantine technical specification

1 Scope

This standard specifies the technique of fluorescent RT-PCR and serum antibody enzyme-linked immunosorbent assay for lymphocytic choriomeningitis virus specification. This standard applies to the diagnosis and epidemiological investigation of lymphocytic choriomeningitis in rats, mice, guinea pigs and hamsters.

2 Normative references

The following documents are indispensable for the application of this document. For dated references, only the dated version applies to this article. Pieces. For undated references, the latest edition (including all amendments) applies to this document. GB 19489 General requirements for laboratory biosafety

3 Abbreviations

The following abbreviations apply to this document. BSA. Serum albumin (Bovineserumalbumin) ELISA. Enzyme-linked immunosorbent assay (Enzyme-linked immunosorbent assay) HRP. Horseradish peroxidase (Horseradish peroxidase) LCMV. Lymphocytic choriomeningitis virus

4 preliminary diagnosis

A preliminary diagnosis can be made by referring to the clinical symptoms and epidemiological data in Appendix A of the disease summary. Further samples should be collected for laboratory use. Detection. The detection of pathogens should be carried out in a secondary biosafety laboratory. Sample storage and waste disposal should be in accordance with GB 19489. get on.

5 Laboratory diagnosis

5.1 Fluorescence quantitative RT-PCR test 5.1.1 Reagents 5.1.1.1 TRIZOL. 5.1.1.2 Trichloromethane. 5.1.1.3 Isopropanol. pre-cooled at -20 °C. 5.1.1.4 0.1 PC water. 5.1.1.5 75% ethanol. 5.1.1.6 RNase inhibitor (40 U/μL). ......

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