SN/T 4739-2016 English PDF

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SN/T 4739-2016: Quarantine protocol for pathogenic Aeromonas hydrophila
Status: Valid

Standard ID	USD	BUY PDF	Lead-Days	Standard Title (Description)	Status
SN/T 4739-2016	329	Add to Cart	3 days	Quarantine protocol for pathogenic Aeromonas hydrophila	Valid

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Basic data

Standard ID: SN/T 4739-2016 (SN/T4739-2016)
Description (Translated English): Quarantine protocol for pathogenic Aeromonas hydrophila
Sector / Industry: Commodity Inspection Standard (Recommended)
Classification of Chinese Standard: B41
Word Count Estimation: 14,180
Date of Issue: 2016-12-12
Date of Implementation: 2017-07-01
Regulation (derived from): State-Quality-Inspection-Accredidation (2016) No.590
Issuing agency(ies): General Administration of Customs

SN/T 4739-2016: Quarantine protocol for pathogenic Aeromonas hydrophila

---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Quarantine protocol for pathogenic Aeromonas hydrophila People's Republic of China Exit Inspection and Quarantine standards Pathogenic Aeromonas hydrophila quarantine technical specifications 2016-12-12 released 2017-07-01 implementation People's Republic of China General Administration of Quality Supervision, Inspection and Quarantine released

Foreword

This standard was drafted in accordance with the rules given in GB/T 1.1-2009. This standard is proposed and managed by the National Certification and Accreditation Administration Committee. This standard was drafted by Jiangsu Entry-Exit Inspection and Quarantine Bureau of People's Republic of China, China Pharmaceutical University. The main drafters of this standard. Jiang Yan, Tong Ming Long, Zhao Xiaoyan, Wang Kai-min, Zhang Yang, Chu Weihua. Pathogenic Aeromonas hydrophila quarantine technical specifications

1 Scope

This standard specifies the isolation and culture of pathogenic Aeromonas hydrophila and polymerase chain reaction detection method. This standard applies to diseased fish and water samples of pathogenic Aeromonas hydrophila separation and identification.

2 Normative references

The following documents for the application of this document is essential. For dated references, only the dated version applies to this article Pieces. For undated references, the latest edition (including all amendments) applies to this document. GB/T 6682 analytical laboratory water specifications and test methods

3 Abbreviations

The following abbreviations apply to this document. PCR. Polymerase Chain Reaction (Polymerase Chain Reaction) Aero. Aeromonas Aerosol SDS. Sodium DodecylSulfate Isolation and identification of pathogenic Aeromonas hydrophila 4.1 Aeromonas hydrophila separation 4.1.1 Medium Preparation 4.1.1.1 Preparation of various media see Appendix A. Test water should meet the GB/T 6682 requirements. 4.1.1.2 Preparation of various reagents see Appendix B. 4.1.2 sample to be tested 4.1.2.1 Uncontaminated materials Aseptic animals take the kidney, liver, spleen and other tissues. 4.1.2.2 contaminated materials. Take contaminated materials such as feces or diseased skin. 4.1.2.3 Water samples Take the sick fish breeding water samples 500mL. 4.1.2.4 Strain The strain is inoculated into ordinary broth (see Appendix A), incubated at 28 ° C for 24 h, and then streaked onto an ordinary agar plate (see Appendix A) to form a single colony for identification purposes. Separation and identification of the flow diagram see Appendix C. 4.1.3 separate culture 4.1.3.1 Unpolluted material separation 4.1.3.1.1 vaccination ring (platinum ear) flame sterilization, cooling. 4.1.3.1.2 dipped with vaccination ring sick material. 4.1.3.1.3 Scribed in ordinary agar plate. ......

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