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SN/T 4675.29-2016 English PDF

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SN/T 4675.29-2016: Determinetion of Brettanomyces bruxellensis in wine for export--Real-time PCR method
Status: Valid

Standard ID	USD	BUY PDF	Lead-Days	Standard Title (Description)	Status
SN/T 4675.29-2016	229	Add to Cart	3 days	Determinetion of Brettanomyces bruxellensis in wine for export--Real-time PCR method	Valid

Similar standards

GB/T 10343 GB/T 394.1 GB 4927 SN/T 4675.32 SN/T 4675.32 SN/T 4675.31

Basic data

Standard ID: SN/T 4675.29-2016 (SN/T4675.29-2016)
Description (Translated English): Determinetion of Brettanomyces bruxellensis in wine for export--Real-time PCR method
Sector / Industry: Commodity Inspection Standard (Recommended)
Classification of Chinese Standard: X62
Word Count Estimation: 10,195
Date of Issue: 2016-12-12
Date of Implementation: 2017-07-01
Regulation (derived from): State-Quality-Inspection-Accredidation (2016) No.590
Issuing agency(ies): General Administration of Customs

SN/T 4675.29-2016: Determinetion of Brettanomyces bruxellensis in wine for export--Real-time PCR method

---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
(A real - time fluorescent PCR method for exporting wine and yeast) People's Republic of China Entry-Exit Inspection and Quarantine Industry Standard Wine yeast test for exporting wine Real-time fluorescent PCR Released on December 12,.2016 2017-07-01 implementation People's Republic The General Administration of Quality Supervision, Inspection and Quarantine issued

Foreword

SN/T 4675 "Methods for Quality and Safety of Exported Wines" is divided into 30 parts. ---SN/T 4675.1. Determination of glycerol in wines; ---SN/T 4675.2. Determination of 2,3-butanediol in exported wines by gas chromatography; ---SN/T 4675.3. Determination of stable carbon isotope ratio of ethanol in exported wines; ---SN/T 4675.4. Determination of lactic acid in exported wines; --- SN/T 4675.5. Determination of organic acids in exported wines by ion chromatography; ---SN/T 4675.6. Determination of glucose, fructose and sucrose in exported wines; ---SN/T 4675.7. Determination of acetaldehyde in exported wines by gas chromatography-mass spectrometry; ---SN/T 4675.8. Determination of 5-hydroxymethylfurfural in exported wines by liquid chromatography; ---SN/T 4675.9. Determination of diethylene glycol in exported wines by gas chromatography-mass spectrometry; ---SN/T 4675.10. Determination of ochratoxin A in exported wines by liquid chromatography-mass spectrometry/mass spectrometry; ---SN/T 4675.11. Determination of seven anthocyanins in exported wines by ultra performance liquid chromatography; ---SN/T 4675.12. Determination of lysozyme in exported wines by liquid chromatography; --- SN/T 4675.13. Determination of 2,4,6-trichloroanisole residues in exported wines by gas chromatography-mass spectrometry; ---SN/T 4675.14. Determination of natamycin in exported wines by liquid chromatography-mass spectrometry/mass spectrometry; ---SN/T 4675.15. Determination of salicylic acid, dehydroacetic acid and p-chlorobenzoic acid in exported wines by liquid chromatography; ---SN/T 4675.16. Determination of fumaric acid in exported wines by liquid chromatography-mass spectrometry/mass spectrometry; --- SN/T 4675.17. Determination of butyl tin content in exported wines by gas chromatography - mass spectrometry/mass spectrometry; --- SN/T 4675.18. Determination of residual amount of dithiocarbamate in exported wines. ---SN/T 4675.19. Determination of sodium, magnesium, potassium, calcium, chromium, manganese, iron, copper, zinc, arsenic, selenium, silver, cadmium and lead in exported wine; ---SN/T 4675.20. Determination of rare earth elements in exported wines by inductively coupled plasma mass spectrometry; --- SN/T 4675.21. Determination of soluble inorganic salts in exported wines by ion chromatography; ---SN/T 4675.22. Determination of total sulfur dioxide in exported wine colorimetric method; ---SN/T 4675.23. Determination of ammonia nitrogen in exported wine and grape juice by continuous flow analyzer method; ---SN/T 4675.24. Determination of the export wine Fulin-Shoka index spectrophotometer method; ---SN/T 4675.25. Determination of color of exported wines CIE1976 (L*a*b*) color space method; ---SN/T 4675.26. Determination of turbidity of exported wines by scattered light method; ---SN/T 4675.27. Determination of alkaline ash in exported wines; ---SN/T 4675.28. Counting bacteria, mold and yeast for exporting wine; ---SN/T 4675.29. Real-time fluorescent PCR method for wine yeast test in exported wine; ---SN/T 4675.30. Real-time fluorescent PCR method for the detection of Bayesian yeast in exported wine. This part is the 29th part of SN/T 4675. This part is drafted in accordance with the rules given in GB/T 1.1-2009. Please note that some of the contents of this document may involve patents. The issuing organization of this document is not responsible for identifying these patents. This part is proposed and managed by the National Certification and Accreditation Administration. This section drafted by. Shanghai Entry-Exit Inspection and Quarantine Bureau of the People's Republic of China, Qinhuangdao Entry and Exit Inspection and Quarantine Bureau, Guangdong Entry-Exit Inspection and Quarantine Bureau of the People's Republic of China. The main drafters of this section. Liu Xia, Li Xiaohong, Wang Chuanxian, Qian Yunkai, Li Zhiyong, Song Qing, Liu Qing, Ling Li, Zhang Wang. Wine yeast test for exporting wine Real-time fluorescent PCR

1 Scope

This part of SN/T 4675 specifies the qualitative detection method for the wine yeast (Brettanomyces bruxellensis). This section applies to the detection of wine yeast in various wines.

2 Normative references

The following documents are indispensable for the application of this document. For dated references, only dated versions apply to this article. Pieces. For undated references, the latest edition (including all amendments) applies to this document. GB/T 6682 Analytical laboratory water specifications and test methods GB 19489 General requirements for laboratory biosafety GB/T 27403 Laboratory Quality Control Specification for Food Molecular Biology Testing

3 Terms and definitions

The following terms and definitions apply to this document. 3.1 Brassica cerevisiae Brettanomycesbruxellensis Yeast is a yeast that can survive in wine and can produce phenols such as 4-ethylphenol and 4-ethyl-2-methoxyphenol. A compound that causes the wine to emit a taste similar to horse sweat. If the concentration is too high, it will seriously affect the quality of the wine.

4 Biosecurity measures and pollution prevention measures

4.1 Biosecurity measures In order to protect the safety of laboratory personnel, it should be tested by qualified staff. All cultures and waste should be referred to. The relevant provisions of GB 19489 are implemented. 4.2 Anti-pollution measures Pollution prevention measures shall comply with the provisions of GB/T 27403.

5 Instruments and equipment

5.1 Water bath or heating template. 95 °C ± 5 °C. 5.2 High-speed refrigerated centrifuge. The highest centrifugal force is not less than 12000g. 5.3 Centrifuge tube. 50mL, 2mL. 5.4 Pipette. range 0.5μL~10μL; range 10μL~100μL; range 100μL~1000μL and sterile tip. ......

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Tips & Frequently Asked Questions:

Question 1: How long will the true-PDF of SN/T 4675.29-2016_English be delivered?

Answer: Upon your order, we will start to translate SN/T 4675.29-2016_English as soon as possible, and keep you informed of the progress. The lead time is typically 1 ~ 3 working days. The lengthier the document the longer the lead time.

Question 2: Can I share the purchased PDF of SN/T 4675.29-2016_English with my colleagues?

Answer: Yes. The purchased PDF of SN/T 4675.29-2016_English will be deemed to be sold to your employer/organization who actually pays for it, including your colleagues and your employer's intranet.

Question 3: Does the price include tax/VAT?

Answer: Yes. Our tax invoice, downloaded/delivered in 9 seconds, includes all tax/VAT and complies with 100+ countries' tax regulations (tax exempted in 100+ countries) -- See Avoidance of Double Taxation Agreements (DTAs): List of DTAs signed between Singapore and 100+ countries

Question 4: Do you accept my currency other than USD?

Answer: Yes. If you need your currency to be printed on the invoice, please write an email to Sales@ChineseStandard.net. In 2 working-hours, we will create a special link for you to pay in any currencies. Otherwise, follow the normal steps: Add to Cart -- Checkout -- Select your currency to pay.