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SN/T 4675.12-2016 English PDF

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US$229.00 · In stock
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SN/T 4675.12-2016: Determination of lysozyme in wine for export - HPLC method
Status: Valid

Standard ID	USD	BUY PDF	Lead-Days	Standard Title (Description)	Status
SN/T 4675.12-2016	229	Add to Cart	3 days	Determination of lysozyme in wine for export - HPLC method	Valid

Similar standards

GB/T 10343 GB/T 394.1 GB 4927 SN/T 4675.32 SN/T 4675.11 SN/T 4675.10

Basic data

Standard ID: SN/T 4675.12-2016 (SN/T4675.12-2016)
Description (Translated English): Determination of lysozyme in wine for export - HPLC method
Sector / Industry: Commodity Inspection Standard (Recommended)
Classification of Chinese Standard: X62
Word Count Estimation: 10,198
Date of Issue: 2016-12-12
Date of Implementation: 2017-07-01
Regulation (derived from): State-Quality-Inspection-Accredidation (2016) No.590
Issuing agency(ies): General Administration of Customs

SN/T 4675.12-2016: Determination of lysozyme in wine for export - HPLC method

---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Determination of lysozyme in wine for export-HPLC method People's Republic of China Exit Inspection and Quarantine standards Determination of lysozyme in exported wine by liquid chromatography 2016-12-12 released 2017-07-01 implementation People's Republic of China General Administration of Quality Supervision, Inspection and Quarantine released

Foreword

SN/T 4675 "export wine quality and safety analysis method" is divided into 30 parts. --- SN/T 4675.1. Determination of glycerol in export wine enzymatic; --- SN/T 4675.2. Determination of 2,3-butanediol in exported wine by gas chromatography; --- SN/T 4675.3. Determination of ethanol stable carbon isotope ratio of exported wine; --- SN/T 4675.4. Determination of lactic acid in export wine enzymatic; --- SN/T 4675.5. Determination of organic acids in exported wine by ion chromatography; --- SN/T 4675.6. Determination of glucose, fructose and sucrose in exported wine; --- SN/T 4675.7. Determination of acetaldehyde in exported wine by gas chromatography - mass spectrometry; --- SN/T 4675.8. Determination of 5-hydroxymethyl furfural in exported wine by liquid chromatography; --- SN/T 4675.9. Determination of diethylene glycol in exported wine by gas chromatography - mass spectrometry; --- Determination of ochratoxin A in export wines - Liquid chromatography - mass spectrometry/mass spectrometry; --- SN/T 4675.11. Determination of 7 kinds of anthocyanins in exported wine by ultra performance liquid chromatography; --- SN/T 4675.12. Determination of lysozyme in wine exports by liquid chromatography; --- SN/T 4675.13. Determination of 2,4,6-trichloro-phenyl ether residues in exported wine by gas chromatography-mass spectrometry; --- SN/T 4675.14. Determination of natamycin in wine exports by liquid chromatography - mass spectrometry/mass spectrometry; --- SN/T 4675.15. Determination of salicylic acid, dehydroacetic acid and p-chlorobenzoic acid in exported wine by liquid chromatography; --- SN/T 4675.16. Determination of fumaric acid in exported wine by liquid chromatography - mass spectrometry/mass spectrometry; --- SN/T 4675.17. Determination of butyl tin content in exported wine - Gas Chromatography - Mass Spectrometry/Mass Spectrometry; --- SN/T 4675.18; Determination of dithiocarbamate residues in exported wine by headspace gas chromatography; Determination of sodium, magnesium, potassium, calcium, chromium, manganese, iron, copper, zinc, arsenic, selenium, cadmium, lead in exported wine; --- SN/T 4675.20. Determination of rare earth elements in exported wine by inductively coupled plasma mass spectrometry; --- SN/T 4675.21. Determination of soluble inorganic salts in export wine by ion chromatography; --- SN/T 4675.22. Determination of total sulfur dioxide in wine exports by colorimetry; --- SN/T 4675.23. Determination of ammonia nitrogen in export wines and grape juice - Continuous flow analyzer method; --- SN/T 4675.24. Determination of export wine Fulin - schoca index spectrophotometer; --- SN/T 4675.25. Determination of the color of exported wine CIE1976 (L * a * b *) color space method; --- SN/T 4675.26. Determination of turbidity of export wine by scattered light method; --- SN/T 4675.27. Determination of export wine alkaline ash; --- SN/T 4675.28. Exports of wine bacteria, mold and yeast count; --- SN/T 4675.29. Export wine wine yeast test real-time fluorescent PCR; --- SN/T 4675.30. Exported wine Bayer engaged yeast test real-time PCR method. This section is part 12 of SN/T 4675. This section drafted in accordance with GB/T 1.1-2009 given rules. This part of the revision uses the International Grape and Wine Organization (OIV) OIV-MA-AS315-14 "Determination of lysozyme in wine - liquid Phase chromatography. " This section is technically compatible with this method, only the individual content of the OIV method OIV-MA-AS315-14 The editorial changes, the main content of the amendment. --- Optimization of chromatographic conditions. Please note that some of this document may be patentable. The issuing agencies of this document do not bear the responsibility of identifying these patents. This part of the national certification and accreditation supervision and management committee proposed and centralized. This part of the drafting unit. People's Republic of China Guangdong Exit Inspection and Quarantine, People's Republic of China Beijing Exit Inspection and Quarantine Epidemic The main drafters of this section. Pang Shiqi, Liu Qing, Pan Bingzhen, Liang Ruiting, Chen Xiu Ming, Liu Chaoxia, Li Zhiyong, Han Shen, Tian Ling, Hsping. Determination of lysozyme in exported wine by liquid chromatography

1 Scope

This part of SN/T 4675 specifies the method for the determination of lysozyme content in wine by high performance liquid chromatography. This section applies to white wine and sparkling wine Determination of lysozyme content.

2 Normative references

The following documents for the application of this document is essential. For dated references, only the dated version applies to this article Pieces. For undated references, the latest edition (including all amendments) applies to this document. GB/T 6682 analytical laboratory water specifications and test methods

3 method summary

This method only detects the content of lysozyme with enzymatic activity in wine, but has nothing to do with the true activity of the enzyme in the protein. Lysozyme in wine after reversed-phase phenyl column separation, high performance liquid chromatography with fluorescence detector or UV detector, retention time Qualitative, external standard method.

4 Reagents and materials

Unless otherwise specified, the reagents used are of analytical grade, water GB/T 6682 provisions of a water. 4.1 Acetonitrile (C2H3N). Chromatographic purity. 4.2 anhydrous ethanol (C2H6O). Hydrochloric acid (HCL). 4.4 tartaric acid (C4H6O6). 4.5 Trifluoroacetic acid (TFA). 4.6 5% hydrochloric acid solution. Take 5mL hydrochloric acid, water to volume 100mL. 4.7 tartaric acid solution Weigh 1.0g tartaric acid (4.4), with 100mL of anhydrous ethanol was dissolved, the volume of water to 1000mL. 4.8 Lysozyme standard material (CAS. 12650-88-3). 4.9 lysozyme standard stock solution accurately weighed amount of lysozyme standard (4.8), add appropriate tartaric acid solution (4.7) ultrasonic oscillation to charge Divide and dilute to volume, prepare a standard stock solution with a concentration of 2μL and store in a refrigerator at 0 ℃ ~ 4 ℃. 4.10 Lysozyme standard working solution, respectively, the exact amount of lysozyme standard stock solution (4.9), diluted with the sample extract to prepare 5.0mg/L, 10.0mg/L, 20.0mg/L, 25.0mg/L, 40.0mg/L, 50.0mg/L, 60.0mg/L standard working solution, is With current use. 4.11 Microporous membrane .0.22μm, water system.

5 instruments and equipment

5.1 High performance liquid chromatography with fluorescence detector or UV detector. ......

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Tips & Frequently Asked Questions:

Question 1: How long will the true-PDF of SN/T 4675.12-2016_English be delivered?

Answer: Upon your order, we will start to translate SN/T 4675.12-2016_English as soon as possible, and keep you informed of the progress. The lead time is typically 1 ~ 3 working days. The lengthier the document the longer the lead time.

Question 2: Can I share the purchased PDF of SN/T 4675.12-2016_English with my colleagues?

Answer: Yes. The purchased PDF of SN/T 4675.12-2016_English will be deemed to be sold to your employer/organization who actually pays for it, including your colleagues and your employer's intranet.

Question 3: Does the price include tax/VAT?

Answer: Yes. Our tax invoice, downloaded/delivered in 9 seconds, includes all tax/VAT and complies with 100+ countries' tax regulations (tax exempted in 100+ countries) -- See Avoidance of Double Taxation Agreements (DTAs): List of DTAs signed between Singapore and 100+ countries

Question 4: Do you accept my currency other than USD?

Answer: Yes. If you need your currency to be printed on the invoice, please write an email to Sales@ChineseStandard.net. In 2 working-hours, we will create a special link for you to pay in any currencies. Otherwise, follow the normal steps: Add to Cart -- Checkout -- Select your currency to pay.