SN/T 4660-2016 English PDF

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SN/T 4660-2016: Identification technology protocol for deer species
Status: Valid

Standard ID	USD	BUY PDF	Lead-Days	Standard Title (Description)	Status
SN/T 4660-2016	229	Add to Cart	3 days	Identification technology protocol for deer species	Valid

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Basic data

Standard ID: SN/T 4660-2016 (SN/T4660-2016)
Description (Translated English): Identification technology protocol for deer species
Sector / Industry: Commodity Inspection Standard (Recommended)
Classification of Chinese Standard: B41
Word Count Estimation: 10,189
Date of Issue: 2016-12-12
Date of Implementation: 2017-07-01
Regulation (derived from): State-Quality-Inspection-Accredidation (2016) No.590
Issuing agency(ies): General Administration of Customs

SN/T 4660-2016: Identification technology protocol for deer species

---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Identification technology protocol for deer species China's entry-exit inspection and quarantine industry standards Deer species identification specification Published on.2016-12-12 2017-07-01 Implementation China The State Administration of Quality Supervision, Inspection and Quarantine issued

Foreword

This standard was drafted in accordance with the rules given in GB/T 1.1-2009. This standard is proposed and managed by the National Certification and Accreditation Regulatory Commission. This standard was drafted by. Liaoning Entry-Exit Inspection and Quarantine Bureau of the People's Republic of China, Institute of Special Products of the Chinese Academy of Agricultural Sciences, Jilin Agriculture University, Jilin Province, China-Korea Animal Science Institute. The main drafters of this standard. Jia Wei, Yan Shen Shen, Sun Mingying, Wang Quankai, Zhang Xue, Song Dahe, Wang Kaiying, Xu Chao, Hu Chuanwei, Yuan Wenze. Deer species identification specification

1 Scope

This standard specifies the PCR identification method for spot deer and red deer species. This standard applies to species identification of spotted deer, red deer, and their products.

2 Normative references

The following documents are indispensable for the application of this document. For dated references, only dated versions apply to this article Pieces. For undated references, the latest version (including all amendments) applies to this document. GB/T 6682 Analysis Laboratory Water Specifications and Test Methods

3 Methodology

According to the published mitochondrial genome sequences of the deer and red deer in the GenBank database, they are conserved within the species and interspecies Select specific regions with specificity, design and synthesize 3 primers. After the sample has been extracted from the total genomic DNA, use special design features. The heterologous primers were used for PCR amplification of the target genes of spotted deer and red deer, and whether the samples contained spotted deer were determined based on the results of PCR amplification. Red deer gene.

4 Morphological features

Deer and red deer morphological characteristics see Appendix A

5 PCR detection

5.1 Major Instruments and Equipment PCR machines, tissue grinders, ultra-clean benches, ice makers, high-speed refrigerated centrifuges, water baths, bench top centrifuges, conventional refrigerators, Vortex oscillator, electrophoresis apparatus, gel imaging system, micro-adjustable pipette (10 μL, 100 μL, 1000 μL) and matching tips. 5.2 Reagents and Materials Unless otherwise specified, the reagents used in this standard are of analytical grade, and the test water should meet the requirements of GB/T 6682. 5.2.1 anhydrous ethanol. 5.2.2 distilled water (should be in line with the specifications of the first-grade water in GB/T 6682). 5.2.3 Protease K (20 mmol/μL). 5.2.4 10% sodium dodecyl sulfate (SDS). 5.2.5 Cetyltriethylammonium bromide (CTAB). 5.2.6 Sodium chloride. 5.2.7 Trichloromethane. 5.2.8 Isopropyl alcohol. ......

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