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SN/T 4649-2016 English PDF

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SN/T 4649-2016: Detection and identification of Didymella bryoniae (Auersw. ) Rehm
Status: Valid
Standard IDUSDBUY PDFLead-DaysStandard Title (Description)Status
SN/T 4649-2016329 Add to Cart 3 days Detection and identification of Didymella bryoniae (Auersw. ) Rehm Valid

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Basic data

Standard ID: SN/T 4649-2016 (SN/T4649-2016)
Description (Translated English): Detection and identification of Didymella bryoniae (Auersw. ) Rehm
Sector / Industry: Commodity Inspection Standard (Recommended)
Classification of Chinese Standard: B16
Word Count Estimation: 14,113
Date of Issue: 2016-08-23
Date of Implementation: 2017-03-01
Regulation (derived from): State-Quality-Inspection-Certification (2016)438
Issuing agency(ies): General Administration of Customs

SN/T 4649-2016: Detection and identification of Didymella bryoniae (Auersw. ) Rehm

---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
(Methods of quarantine and identification of) People's Republic of China Entry-Exit Inspection and Quarantine Industry Standard Quarantine and identification method for melon blight Published on.2016-08-23 2017-03-01 implementation People's Republic The General Administration of Quality Supervision, Inspection and Quarantine issued

Foreword

This standard was drafted in accordance with the rules given in GB/T 1.1-2009. This standard is proposed and managed by the National Certification and Accreditation Administration. This standard is drafted by. Xinjiang Entry-Exit Inspection and Quarantine Bureau of the People's Republic of China, Shihezi University. The main drafters of this standard. Wang Wei, Zhang Xiaoju, Sun Yanfei, Zhang Xianglin, Zhang Wei, Li Yawei, Zhang Yu. Quarantine and identification method for melon blight

1 Scope

This standard specifies the quarantine and identification methods for the genus Didymellabryoniae (Auersw.) Rehm. This standard is applicable to the quarantine and identification of melon fungus bacteria in seeds, plants and fruits of import and export melon plants.

2 Normative references

The following documents are indispensable for the application of this document. For dated references, only dated versions apply to this article. Pieces. For undated references, the latest edition (including all amendments) applies to this document. SN/T 1809 In and out plant seed quarantine regulations

3 Basic information on the fungus

Scientific name. Didymellabryoniae (Auersw.) Rehm Asexual generation scientific name. Ascochytacitrullina (Chester) Smith Sexual Generation. Didymellabryoniae (Auersw.) Rehm Classification status. Ascomycota, Pleosporales, and Didy- Mosphaeriaceae), Didymella; asexuality Sphaeropsidales, Sphaeropsi- Caceae), Ascochyta.

4 Principle of the method

Symptoms, culture traits, morphological characteristics, pathogenicity and PCR-specific amplification test results of Phytophthora infestans Basis for quarantine identification of bacteria.

5 instruments and main reagents

5.1 Instrumentation Biological microscope, stereoscopic mirror, high-speed refrigerated centrifuge, pure water meter, ultra-clean workbench, autoclave, ice machine, PCR instrument, condensation Glue imaging system, electrophoresis instrument, incubator, ultra-low temperature refrigerator, conventional refrigerator, vortex oscillator. 5.2 main reagent All reagents are either analytical or biochemical reagents unless otherwise stated. 1% sodium hypochlorite, DNA extraction related reagents (box), PCR related reagents, electrophoresis reagents. Potato dextrose agar medium (PDA) (see Appendix A for preparation methods).
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