SN/T 4647-2016 English PDFUS$419.00 · In stock
Delivery: <= 4 days. True-PDF full-copy in English will be manually translated and delivered via email. SN/T 4647-2016: Detection and identification of Phytophthora fragariae Hickman Status: Valid
Basic dataStandard ID: SN/T 4647-2016 (SN/T4647-2016)Description (Translated English): Detection and identification of Phytophthora fragariae Hickman Sector / Industry: Commodity Inspection Standard (Recommended) Classification of Chinese Standard: B16 Word Count Estimation: 18,113 Date of Issue: 2016-08-23 Date of Implementation: 2017-03-01 Regulation (derived from): State-Quality-Inspection-Certification (2016)438 Issuing agency(ies): General Administration of Customs SN/T 4647-2016: Detection and identification of Phytophthora fragariae Hickman---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.(Method for quarantine and identification of Phytophthora capsici) People's Republic of China Entry-Exit Inspection and Quarantine Industry Standard Quarantine and identification method of Phytophthora infestans Published on.2016-08-23 2017-03-01 implementation People's Republic The General Administration of Quality Supervision, Inspection and Quarantine issued ForewordThis standard was drafted in accordance with the rules given in GB/T 1.1-2009. This standard is proposed and managed by the National Certification and Accreditation Administration. This standard was drafted. Hubei Entry-Exit Inspection and Quarantine Bureau of the People's Republic of China, Nansha Entry-Exit Inspection and Quarantine Bureau, Jilin Entry-Exit Inspection and Quarantine Bureau of the People's Republic of China, Jiangsu Entry-Exit Inspection and Quarantine Bureau of the People's Republic of China, Shandong Entry-Exit Inspection and Quarantine Bureau, Shenzhen Entry-Exit Inspection and Quarantine Bureau of the People's Republic of China. The main drafters of this standard. Wang Zhenhua, Ye Wei, Chen Ping, Li Jinxi, Xu Xue, Wei Chunyan, Li Bin, Xu Jiawen, Wang Yingchao, Chen Jianjun, Zeng Xiandong, Chen Xiaoyu, Cheng Yinghui, Zheng Jian. Quarantine and identification method of Phytophthora infestans1 ScopeThis standard specifies the quarantine and identification methods for Phytophthorafragariae Hickman. This standard is applicable to the quarantine and identification of Phytophthora infestans on the roots and fruits of strawberry seedlings in and out of the country.2 Normative referencesThe following documents are indispensable for the application of this document. For dated references, only the dated version applies to this document. Pieces. For undated references, the latest edition (including all amendments) applies to this document. GB/T 6682 Analytical laboratory water specifications and test methods3 Basic information on Phytophthora infestansScientific name. PhytophthorafragariaeHickman Classification status. Phytophthora infestans, Chromista, Oomycota, Peronosporea, Peronosporales, Peronosporaceae, Phytophthora. Phytophthorafragariae has 2 variants. PhytophthorafragariaeHickmanvar.fragariaeWilcox gariaeHickmanvar.rubiWilcox See Appendix A for additional information on Phytophthora infestans.4 Principle of the methodAccording to the symptoms, morphological characteristics and PCR-specific amplified fragments of Phytophthora infestans on the host, the identification basis was used.5 instruments and main reagents5.1 Instrumentation Biological microscope, stereo microscope, plant growth incubator, high speed refrigerated centrifuge, pure water meter, biological safety cabinet, sterilization pot, ice machine, PCR instrument, gel imaging system, electrophoresis instrument, incubator, ultra-low temperature refrigerator, conventional refrigerator, vortex oscillator. 5.2 Main reagents and medium All reagents were of analytical grade unless otherwise stated. The experimental water should meet the requirements of GB/T 6682. 5.2.1 1% sodium hypochlorite. 5.2.2 DNA extraction reagent. DNA extraction kit. Isopropanol, absolute ethanol, proteinase K (20 mg/mL), phenol. chloroform. A solution of isoamyl alcohol (25.24.1), 3 mol/L NaAc (pH 5.2). 5.2.3 PCR reagent. 10×PCR buffer (containing 25mmol/L MgSO4), Taq DNA polymerase, dNTPs (dATP, ......Tips & Frequently Asked Questions:Question 1: How long will the true-PDF of SN/T 4647-2016_English be delivered?Answer: Upon your order, we will start to translate SN/T 4647-2016_English as soon as possible, and keep you informed of the progress. The lead time is typically 2 ~ 4 working days. The lengthier the document the longer the lead time.Question 2: Can I share the purchased PDF of SN/T 4647-2016_English with my colleagues?Answer: Yes. The purchased PDF of SN/T 4647-2016_English will be deemed to be sold to your employer/organization who actually pays for it, including your colleagues and your employer's intranet.Question 3: Does the price include tax/VAT?Answer: Yes. 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