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SN/T 4647-2016 English PDF

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SN/T 4647-2016: Detection and identification of Phytophthora fragariae Hickman
Status: Valid
Standard IDUSDBUY PDFLead-DaysStandard Title (Description)Status
SN/T 4647-2016419 Add to Cart 4 days Detection and identification of Phytophthora fragariae Hickman Valid

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Basic data

Standard ID: SN/T 4647-2016 (SN/T4647-2016)
Description (Translated English): Detection and identification of Phytophthora fragariae Hickman
Sector / Industry: Commodity Inspection Standard (Recommended)
Classification of Chinese Standard: B16
Word Count Estimation: 18,113
Date of Issue: 2016-08-23
Date of Implementation: 2017-03-01
Regulation (derived from): State-Quality-Inspection-Certification (2016)438
Issuing agency(ies): General Administration of Customs

SN/T 4647-2016: Detection and identification of Phytophthora fragariae Hickman

---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
(Method for quarantine and identification of Phytophthora capsici) People's Republic of China Entry-Exit Inspection and Quarantine Industry Standard Quarantine and identification method of Phytophthora infestans Published on.2016-08-23 2017-03-01 implementation People's Republic The General Administration of Quality Supervision, Inspection and Quarantine issued

Foreword

This standard was drafted in accordance with the rules given in GB/T 1.1-2009. This standard is proposed and managed by the National Certification and Accreditation Administration. This standard was drafted. Hubei Entry-Exit Inspection and Quarantine Bureau of the People's Republic of China, Nansha Entry-Exit Inspection and Quarantine Bureau, Jilin Entry-Exit Inspection and Quarantine Bureau of the People's Republic of China, Jiangsu Entry-Exit Inspection and Quarantine Bureau of the People's Republic of China, Shandong Entry-Exit Inspection and Quarantine Bureau, Shenzhen Entry-Exit Inspection and Quarantine Bureau of the People's Republic of China. The main drafters of this standard. Wang Zhenhua, Ye Wei, Chen Ping, Li Jinxi, Xu Xue, Wei Chunyan, Li Bin, Xu Jiawen, Wang Yingchao, Chen Jianjun, Zeng Xiandong, Chen Xiaoyu, Cheng Yinghui, Zheng Jian. Quarantine and identification method of Phytophthora infestans

1 Scope

This standard specifies the quarantine and identification methods for Phytophthorafragariae Hickman. This standard is applicable to the quarantine and identification of Phytophthora infestans on the roots and fruits of strawberry seedlings in and out of the country.

2 Normative references

The following documents are indispensable for the application of this document. For dated references, only the dated version applies to this document. Pieces. For undated references, the latest edition (including all amendments) applies to this document. GB/T 6682 Analytical laboratory water specifications and test methods

3 Basic information on Phytophthora infestans

Scientific name. PhytophthorafragariaeHickman Classification status. Phytophthora infestans, Chromista, Oomycota, Peronosporea, Peronosporales, Peronosporaceae, Phytophthora. Phytophthorafragariae has 2 variants. PhytophthorafragariaeHickmanvar.fragariaeWilcox gariaeHickmanvar.rubiWilcox See Appendix A for additional information on Phytophthora infestans.

4 Principle of the method

According to the symptoms, morphological characteristics and PCR-specific amplified fragments of Phytophthora infestans on the host, the identification basis was used.

5 instruments and main reagents

5.1 Instrumentation Biological microscope, stereo microscope, plant growth incubator, high speed refrigerated centrifuge, pure water meter, biological safety cabinet, sterilization pot, ice machine, PCR instrument, gel imaging system, electrophoresis instrument, incubator, ultra-low temperature refrigerator, conventional refrigerator, vortex oscillator. 5.2 Main reagents and medium All reagents were of analytical grade unless otherwise stated. The experimental water should meet the requirements of GB/T 6682. 5.2.1 1% sodium hypochlorite. 5.2.2 DNA extraction reagent. DNA extraction kit. Isopropanol, absolute ethanol, proteinase K (20 mg/mL), phenol. chloroform. A solution of isoamyl alcohol (25.24.1), 3 mol/L NaAc (pH 5.2). 5.2.3 PCR reagent. 10×PCR buffer (containing 25mmol/L MgSO4), Taq DNA polymerase, dNTPs (dATP,
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