SN/T 4631-2016 English PDF

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SN/T 4631-2016: Detection and identification of Narcissus common latent virus
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Standard ID	USD	BUY PDF	Lead-Days	Standard Title (Description)	Status
SN/T 4631-2016	329	Add to Cart	3 days	Detection and identification of Narcissus common latent virus	Valid

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Basic data

Standard ID: SN/T 4631-2016 (SN/T4631-2016)
Description (Translated English): Detection and identification of Narcissus common latent virus
Sector / Industry: Commodity Inspection Standard (Recommended)
Classification of Chinese Standard: B16
Word Count Estimation: 14,177
Date of Issue: 2016-08-23
Date of Implementation: 2017-03-01
Regulation (derived from): State-Quality-Inspection-Certification (2016)438
Issuing agency(ies): General Administration of Customs

SN/T 4631-2016: Detection and identification of Narcissus common latent virus

---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Detection and identification of Narcissus common latent virus People's Republic of China Exit Inspection and Quarantine standards Narcissus common latent virus quarantine and identification methods 2016-08-23 released 2017-03-01 Implementation People's Republic of China General Administration of Quality Supervision, Inspection and Quarantine released

Foreword

This standard was drafted in accordance with the rules given in GB/T 1.1-2009. This standard is proposed and managed by the National Certification and Accreditation Administration Committee. This standard was drafted by Fujian Entry-Exit Inspection and Quarantine of People's Republic of China, Xiamen Entry-Exit Inspection and Quarantine of the People's Republic of China Bureau, Institute of Biotechnology, Chinese Academy of Agricultural Sciences, China Academy of Inspection and Quarantine, Zhejiang Academy of Inspection and Quarantine Science and Technology, Fujian Agriculture and Forestry University. The main drafters of this standard. Shen Jianguo, Liao Fu Rong, Li Min, Li Weimin, Zhang Yongjiang, Shao Biying, Liu Zhengcai, Wu Ying, Gao Fangluan, Wu Zujian. Narcissus common latent virus quarantine and identification methods

1 Scope

This standard specifies the Narcissus common latent virus quarantine methods. This standard applies to immigration Narcissus scales corm, plants Narcissus common latent virus detection.

2 Normative references

The following documents for the application of this document is essential. For dated references, only the dated version applies to this article Pieces. For undated references, the latest edition (including all amendments) applies to this document. GB/T 6682 analytical laboratory water specifications and test methods SN/T 2122 Quarantine and sampling methods for entry and exit of plants and plant products SN/T 2964 plant virus detection specification SN/T 3457 Codex specification for plant virus molecular biology

3 principle

The serological and molecular biological properties of Narcissus common latent virus (see Appendix A for background information) are the main To be based. 4 equipment, appliances and reagents 4.1 Equipment High-speed refrigerated centrifuge, electronic balance (1/1000g), PCR instrument, electrophoresis apparatus, horizontal electrophoresis tank, -20 ℃ low temperature refrigerator and -80 ℃ Ultra-low temperature refrigerator, gel imaging analysis system, pH meter, microwave oven, magnetic stirrer, constant temperature water bath, autoclave, clean bench, enzyme Marker and so on. 4.2 appliances Adjustable pipettes of various ranges (1000 μL,.200 μL, 100 μL, 20 μL, 10 μL, 2 μL), PCR tubes, RNase-free Eppendorf centrifuge tube (1.5 mL), mortar, ELISA plate and the like. 4.3 Reagents Unless otherwise specified, all reagents are of analytical grade, the experimental water should be consistent with the relevant provisions of GB/T 6682. ELISA plate capture antigen See Appendix B for plate-trapped antigen ELISA (PTA-ELISA) reagents, see RT-PCR Reagents see Appendix C, real-time fluorescence RT-PCR detection reagents see Appendix D.

5 Sampling and sample preparation

5.1 Sampling Try to extract the virus damage the symptoms of materials, NCLV hazards described in Appendix A; Sampling methods in accordance with SN/T 2122 ......

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