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SN/T 4624.8-2016 English PDF

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SN/T 4624.8-2016: Methods for examination of import microbial blends in the environmental protection--Part 8: Shigella
Status: Valid
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SN/T 4624.8-2016509 Add to Cart 4 days Methods for examination of import microbial blends in the environmental protection--Part 8: Shigella Valid

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Basic data

Standard ID: SN/T 4624.8-2016 (SN/T4624.8-2016)
Description (Translated English): Methods for examination of import microbial blends in the environmental protection--Part 8: Shigella
Sector / Industry: Commodity Inspection Standard (Recommended)
Classification of Chinese Standard: Z04
Word Count Estimation: 22,295
Date of Issue: 2016-08-23
Date of Implementation: 2017-03-01
Regulation (derived from): State-Quality-Inspection-Certification (2016)438
Issuing agency(ies): General Administration of Customs

SN/T 4624.8-2016: Methods for examination of import microbial blends in the environmental protection--Part 8: Shigella


---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
(Test method for microbial inoculants for entry into the environment - Part 8. Shigella) People's Republic of China Entry-Exit Inspection and Quarantine Industry Standard Method for detecting microbial agents for environmental protection Part 8. Shigella protection-Part 8. Shigella Published on.2016-08-23 2017-03-01 implementation People's Republic The General Administration of Quality Supervision, Inspection and Quarantine issued

Foreword

SN/T 4624 "Method for the detection of microbial agents for environmental protection in entry" is divided into 17 parts. --- Part 1. Bacillus licheniformis; --- Part 2. Bacillus pumilus; --- Part 3. Bacillus megaterium; --- Part 4. Thiobacillus ferrooxidans; ---Part 5. β-type hemolytic streptococcus; --- Part 6. Staphylococcus aureus; --- Part 7. Salmonella; --- Part 8. Shigella; --- Part 9. Escherichia coli; --- Part 10. Paecilomyces lilacinus; --- Part 11. elegant small gram silver mold; --- Part 12. Trichoderma harzian; --- Part 13. Phanerochaete chrysosporium; --- Part 14. Aspergillus oryzae; ---Part 15. Bacillus amyloliquefaciens; --- Part 16. Pseudomonas aeruginosa; --- Part 17. Pseudomonas putida. This part is the eighth part of SN/T 4624. This part is drafted in accordance with the rules given in GB/T 1.1-2009. This part is proposed and managed by the National Certification and Accreditation Administration. This section drafted by. Liaoning Entry-Exit Inspection and Quarantine Bureau of the People's Republic of China. The main drafters of this standard. Wang Jinling, Yan Shenshun, Zhong Wei, Yu Li, Jin Xuedian, Wang Fang. Method for detecting microbial agents for environmental protection Part 8. Shigella

1 Scope

This part of SN/T 4624 stipulates the morphological identification and biochemical identification of Shigella in the hygiene inspection of microbial agents for environmental protection. Fixed, common PCR, real-time fluorescent PCR detection methods. This section applies to the detection and identification of Shigella in the hygiene inspection of microbial agents for environmental protection.

2 Normative references

The following documents are indispensable for the application of this document. For dated references, only dated versions apply to this article. Pieces. For undated references, the latest edition (including all amendments) applies to this document. GB/T 6682 Analytical laboratory water specifications and test methods

3 main reagents and medium

3.1 Experimental water. It should meet the specifications of the first grade water in GB/T 6682. 3.2 Saline. See Appendix A, A.1. 3.3 Shigella enrichment broth - novobiocin. see A.2. 3.4 MacConkey (MAC) agar. See A.3. 3.5 Xylose Lysine Deoxycholate (XLD) agar. See A.4. 3.6 Shigella chromogenic medium. see A.5. 3.7 Trisaccharide Iron (TSI) Agar. See A.6. 3.8 Nutritional agar slants. see A.7. 3.9 Semi-solid agar. See A.8. 3.10 Ammonium Glucose Medium. See A.9. 3.11 Urea Agar. See A.10. 3.12 β-galactosidase medium. see A.11. 3.13 Amino acid decarboxylase assay medium. see A.12. 3.14 Sugar Fermentation Tube. See A.13. 3.15 Simon's Citrate Medium. See A.14. 3.16 Protein Hydrophobic and Rhodium Matrix Reagents. See A.15. 3.17 Shigella diagnostic serum. 3.18 Biochemical identification kit. 3.19 TE buffer (pH 8.0). see A.16. 3.20 Lysozyme (10 mg/mL). see A.17. 3.21 Proteinase K (20 mg/mL). see A.18. 3.22 10% SDS. See A.19.
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