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SN/T 4624.7-2016 English PDF

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SN/T 4624.7-2016: Methods for examination of import microbial blends in the environmental protection--Part 7: Salmonella
Status: Valid
Standard IDUSDBUY PDFLead-DaysStandard Title (Description)Status
SN/T 4624.7-2016419 Add to Cart 4 days Methods for examination of import microbial blends in the environmental protection--Part 7: Salmonella Valid

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HG/T 5871   GB/T 27611   GB/T 20861   SN/T 4624.18   SN/T 4624.11   SN/T 4624.10   

Basic data

Standard ID: SN/T 4624.7-2016 (SN/T4624.7-2016)
Description (Translated English): Methods for examination of import microbial blends in the environmental protection--Part 7: Salmonella
Sector / Industry: Commodity Inspection Standard (Recommended)
Classification of Chinese Standard: Z04
Word Count Estimation: 18,170
Date of Issue: 2016-08-23
Date of Implementation: 2017-03-01
Regulation (derived from): State-Quality-Inspection-Certification (2016)438
Issuing agency(ies): General Administration of Customs

SN/T 4624.7-2016: Methods for examination of import microbial blends in the environmental protection--Part 7: Salmonella


---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
(Detection of microbial inoculants for entry into the environment - Part 7. Salmonella) People's Republic of China Entry-Exit Inspection and Quarantine Industry Standard Method for detecting microbial agents for environmental protection Part 7. Salmonella protection-Part 7. Salmonella Published on.2016-08-23 2017-03-01 implementation People's Republic The General Administration of Quality Supervision, Inspection and Quarantine issued Entry and exit inspection and quarantine Industry Standard Method for detecting microbial agents for environmental protection Part 7. Salmonella China Standard Press Publishing First edition in December.2017

Foreword

SN/T 4624 "Method for the detection of microbial agents for environmental protection in entry" is divided into 17 parts. --- Part 1. Bacillus licheniformis; --- Part 2. Bacillus pumilus; --- Part 3. Bacillus megaterium; --- Part 4. Thiobacillus ferrooxidans; ---Part 5. β-type hemolytic streptococcus; --- Part 6. Staphylococcus aureus; --- Part 7. Salmonella; --- Part 8. Shigella; --- Part 9. Escherichia coli; --- Part 10. Paecilomyces lilacinus; --- Part 11. elegant small gram silver mold; --- Part 12. Trichoderma harzian; --- Part 13. Phanerochaete chrysosporium; --- Part 14. Aspergillus oryzae; ---Part 15. Bacillus amyloliquefaciens; --- Part 16. Pseudomonas aeruginosa; --- Part 17. Pseudomonas putida. This part is the seventh part of SN/T 4624. This part is drafted in accordance with the rules given in GB/T 1.1-2009. This part is proposed and managed by the National Certification and Accreditation Administration. This section drafted by. Liaoning Entry-Exit Inspection and Quarantine Bureau of the People's Republic of China. The main drafters of this section. Wang Jinling, Yan Shenshun, Liu Yu, Zhong Wei, Liu Ying, Li Wei, Wang Fang. Method for detecting microbial agents for environmental protection Part 7. Salmonella

1 Scope

This part of SN/T 4624 stipulates the morphological identification and biochemical identification of Salmonella in the sanitary inspection of microbial agents for environmental protection. Fixed, common PCR, real-time fluorescent PCR detection methods. This section applies to the detection and identification of Salmonella in the hygiene inspection of microbial agents for environmental protection.

2 Normative references

The following documents are indispensable for the application of this document. For dated references, only dated versions apply to this article. Pieces. For undated references, the latest edition (including all amendments) applies to this document. GB/T 6682 Analytical laboratory water specifications and test methods

3 main reagents and medium

3.1 Experimental water. It should meet the specifications of the first grade water in GB/T 6682. 3.2 Saline. See Appendix A, A.1. 3.3 sodium tetrasulfonate brilliant green (TTB) enrichment solution. see A.2. 3.4 Selenite cystine (SC) enrichment solution. see A.3. 3.5 Barium sulfite (BS) agar. see A.4. 3.6 Xylose Lysine Deoxycholate (XLD) agar. See A.5. 3.7 Salmonella chromogenic medium. 3.8 Trisaccharide Iron (TSI) Agar. See A.6. 3.9 Peptone water, sputum matrix reagent. see A.7. 3.10 Urea agar (pH 7.2). See A.8. 3.11 Potassium cyanide (KCN) medium. see A.9. 3.12 Lysine decarboxylase assay medium. see A.10. 3.13 o-nitrophenol β-D galactoside (ONPG) medium. see A.11. 3.14 Semi-solid agar. See A.12. 3.15 sodium malonate medium. see A.13. 3.16 Salmonella O and H diagnostic serum. 3.17 Biochemical Identification Kit. 3.18 TE buffer (pH 8.0). see A.14. 3.19 Lysozyme (10 mg/mL). see A.15. 3.20 Proteinase K (20 mg/mL). see A.16. 3.21 10% SDS. See A.17. 3.22 3 mol/L sodium acetate. see A.18.
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