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SN/T 4624.12-2016 English PDF

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SN/T 4624.12-2016: Methods for examination of import microbial blends in the environmental protection--Part 12: Trichoderma harzianum
Status: Valid
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SN/T 4624.12-2016229 Add to Cart 3 days Methods for examination of import microbial blends in the environmental protection--Part 12: Trichoderma harzianum Valid

Similar standards

HG/T 5871   GB/T 27611   GB/T 20861   SN/T 4624.20   SN/T 4624.18   SN/T 4624.10   

Basic data

Standard ID: SN/T 4624.12-2016 (SN/T4624.12-2016)
Description (Translated English): Methods for examination of import microbial blends in the environmental protection--Part 12: Trichoderma harzianum
Sector / Industry: Commodity Inspection Standard (Recommended)
Classification of Chinese Standard: Z04
Word Count Estimation: 10,115
Date of Issue: 2016-08-23
Date of Implementation: 2017-03-01
Regulation (derived from): State-Quality-Inspection-Certification (2016)438
Issuing agency(ies): General Administration of Customs

SN/T 4624.12-2016: Methods for examination of import microbial blends in the environmental protection--Part 12: Trichoderma harzianum


---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Methods for examination of import microbial blends in the environmental protection--Part 12. Trichoderma harzianum China's entry-exit inspection and quarantine industry standards Environment-friendly microbial agents for detection Part 12. Trichoderma harzianum protection-Part 12. Trichodermaharzianum Published on.2016-08-23 2017-03-01 Implementation China The State Administration of Quality Supervision, Inspection and Quarantine issued Inspection and Quarantine of the People's Republic of China Industry Standard Environment-friendly microbial agents for detection Part 12. Trichoderma harzianum China Standard Press Publishing The first edition of November.2017

Foreword

SN/T 4624 "Environmental Use of Microbial Agents for Environmental Protection" is divided into 17 parts. --- Part 1. Bacillus licheniformis; --- Part 2. Bacillus pumilus; --- Part 3. Bacillus megaterium; --- Part 4. Thiobacillus ferrooxidans; --- Part 5. Beta hemolytic streptococci; --- Part 6. Staphylococcus aureus; --- Part 7. Salmonella; --- Part 8. Shigella; --- Part 9. Escherichia coli; --- Part 10. Paecilomyces lilacinus; --- Part 11. Elegant Chickwort; --- Part 12. Trichoderma harzianum --- Part 13. Phanerochaete chrysosporium; --- Part 14. Aspergillus coli; --- Part 15. Bacillus amyloliquefaciens; --- Part 16. Pseudomonas alkaloids; --- Part 17. Pseudomonas putida. This section is Part 12 of SN/T 4624. This section was drafted in accordance with the rules given in GB/T 1.1-2009. This part is proposed and managed by the National Certification and Accreditation Regulatory Commission. This section was drafted by. Liaoning Entry-Exit Inspection and Quarantine Bureau of the People's Republic of China and Jilin Entry-Exit Inspection and Quarantine Bureau. The main drafters of this section. Zhang Ying, Liu Yang, Yan Chengcheng, Zhang Zhenyu, Wu Jieyuan, Yang Manhui, and Wang Fang. Environment-friendly microbial agents for detection Part 12. Trichoderma harzianum

1 Scope

This part of SN/T 4624 stipulates the morphological identification and real-time fluorescence of Trichoderma harzianum for the purpose of confirming the conformity of environmental microorganisms used for entry into the country. PCR detection method. This section applies to the entry of environmentally friendly microbial agents for the detection of Harmful Hartz test and identification.

2 Normative references

The following documents are indispensable for the application of this document. For dated references, only dated versions apply to this article In the case of undated references, the latest version (including all amendments) applies to this document. GB/T 6682 Analysis Laboratory Water Specifications and Test Methods

3 Main reagents and culture media

3.1 The test water should meet the specifications of the first-grade water in GB/T 6682. 3.2 Potato-dextrose agar (PDA). See Appendix A, A.1. 3.3 Potato-dextrose broth. See A.2. 3.4 CTAB buffer. see A.3. 3.5 Proteinase K. 3.6 Tris saturated phenol. 3.7 Chloroform. 3.8 Isopentanol. 3.9 Isopropyl alcohol. 3.10 TE buffer (pH 8.0). See A.4. 3.11 70% ethanol. 3.12 Ethidium bromide. 3.13 DNA molecular weight markers. 1000 bp DNA ladder. 3.14 Agarose. 3.15 50 x TAE buffer (pH 8.5). see A.5. 3.16 RealMasterMix (2.5x with ROX internal reference dye). 3.17 20×ProbeEnhancerSolution. 3.18 liquid nitrogen.

4 Major equipment and materials

4.1 Electronic balance (sensibility 0.01g). 4.2 Incubator. 25 °C ± 1 °C.
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