SN/T 4615-2016 English PDFUS$229.00 · In stock
Delivery: <= 3 days. True-PDF full-copy in English will be manually translated and delivered via email. SN/T 4615-2016: Detection method for human infected avian influenza A (H7N9) virus by real time RT-PCR at frontier ports Status: Valid
Basic dataStandard ID: SN/T 4615-2016 (SN/T4615-2016)Description (Translated English): Detection method for human infected avian influenza A (H7N9) virus by real time RT-PCR at frontier ports Sector / Industry: Commodity Inspection Standard (Recommended) Classification of Chinese Standard: C62 Word Count Estimation: 10,112 Date of Issue: 2016-08-23 Date of Implementation: 2017-03-01 Regulation (derived from): State-Quality-Inspection-Certification (2016)438 Issuing agency(ies): General Administration of Customs SN/T 4615-2016: Detection method for human infected avian influenza A (H7N9) virus by real time RT-PCR at frontier ports---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order. Detection method for human infected avian influenza A (H7N9) virus by real time RT-PCR at frontier ports China's entry-exit inspection and quarantine industry standards People at border ports infected with H7N9 bird flu virus in real time Fluorescent RT-PCR detection method Published on.2016-08-23 2017-03-01 Implementation China The State Administration of Quality Supervision, Inspection and Quarantine issued ForewordThis standard was drafted in accordance with the rules given in GB/T 1.1-2009. This standard is proposed and managed by the National Certification and Accreditation Regulatory Commission. This standard grass unit. China Academy of Inspection and Quarantine. The main drafters of this standard are. Hu Kongxin, Ma Xuezheng, Zhang Liping, Sun Xiaohong, and Liu Jian. People at border ports infected with H7N9 bird flu virus in real time Fluorescent RT-PCR detection method1 ScopeThis standard specifies the biosafety requirements for the detection of H7N9 bird flu virus infections at the frontier ports, collection, transportation and preservation of specimens. Specimen processing, human infection H7N9 bird flu real-time fluorescence RT-PCR detection method. This standard is applicable to the detection of nucleic acid in the H7N9 bird flu virus infected by persons entering or exiting the border port.2 Normative referencesThe following documents are indispensable for the application of this document. For dated references, only dated versions apply to this article Pieces. For undated references, the latest version (including all amendments) applies to this document. GB 19489 General Requirements for Laboratory Biosafety WS233 General guidelines for biosafety in microbiological and biomedical laboratories Regulations for the Transportation of High Pathogenic Pathogenic Microorganisms (Poison) Species or Samples that Can Infect Humans (Order of the Ministry of Health of the People's Republic of China) No. 45))3 Terms and DefinitionsThe following terms and definitions apply to this document. 3.1 Human infection of the H7N9 bird flu virus Humaninfectedavianinfluenza A (H7N9) A subtype of the avian influenza virus to which the orthomyxoviridae belong, which is a novel reassortant virus. Due to bird flu virus and human flu disease There are receptor-specific differences in the virus, although occasionally there are reports of certain H7 viruses (H7N2, H7N3, H7N7) infecting humans, which are generally considered Bird flu is not easy to infect, but after the genetic reorganization of influenza viruses in recent years, some highly pathogenic avian flu that have spread among poultry. Subtypes and newly formed subtypes of bird flu have been able to break through germline infections in humans. 3.2 Real-time fluorescence RT-PCR realtime fluorescence RT-PCR On the basis of conventional RT-PCR, a specific fluorescent probe was added. The probe is a piece of oligonucleotide labeled at both ends One reporter group and one quenched fluorophore. When the probe is intact, the fluorescence signal emitted by the reporter group is absorbed by the quencher group; At the time of growth, the probe was digested and degraded by the 5′-3′ exonuclease activity of the Taq enzyme, so that the fluorescent group and the quenching group were separated from each other. The measurement system can receive fluorescent signals. The number of cycles that the fluorescence signal in each reaction tube reaches the set threshold, using the Ct value Indicated.4 AbbreviationsThe following abbreviations apply to this document. 4.1 RT-PCR. reverse transcription-polymerase chain reaction ......Tips & Frequently Asked Questions:Question 1: How long will the true-PDF of SN/T 4615-2016_English be delivered?Answer: Upon your order, we will start to translate SN/T 4615-2016_English as soon as possible, and keep you informed of the progress. The lead time is typically 1 ~ 3 working days. 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