SN/T 4613-2016 English PDF

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SN/T 4613-2016: Real-time RT-PCR method for Kunjin virus detection at frontier ports
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Standard ID	USD	BUY PDF	Lead-Days	Standard Title (Description)	Status
SN/T 4613-2016	139	Add to Cart	3 days	Real-time RT-PCR method for Kunjin virus detection at frontier ports	Valid

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Basic data

Standard ID: SN/T 4613-2016 (SN/T4613-2016)
Description (Translated English): Real-time RT-PCR method for Kunjin virus detection at frontier ports
Sector / Industry: Commodity Inspection Standard (Recommended)
Classification of Chinese Standard: C62
Word Count Estimation: 6,614
Date of Issue: 2016-08-23
Date of Implementation: 2017-03-01
Regulation (derived from): State-Quality-Inspection-Certification (2016)438
Issuing agency(ies): General Administration of Customs

SN/T 4613-2016: Real-time RT-PCR method for Kunjin virus detection at frontier ports

---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Real-time RT-PCR method for Kunjin virus detection at frontier ports China's entry-exit inspection and quarantine industry standards Quantitative RT-PCR detection method for Kunjin virus at border ports Published on.2016-08-23 2017-03-01 Implementation China The State Administration of Quality Supervision, Inspection and Quarantine issued Inspection and Quarantine of the People's Republic of China Industry Standard Quantitative RT-PCR detection method for Kunjin virus at border ports China Standard Press Publishing First edition of January.2018

Foreword

This standard was drafted in accordance with the rules given in GB/T 1.1-2009. This standard is proposed and managed by the National Certification and Accreditation Regulatory Commission. This standard was drafted by. China Academy of Inspection and Quarantine, Hebei International Travel Health Care Center. The main drafters of this standard. Sun Xiaohong, Liu Jian, Yan Yihuan, Xu Baoliang, Ma Xuezheng, Zhang Liping, Bai Hongyan. Quantitative RT-PCR detection method for Kunjin virus at border ports

1 Scope

This standard specifies the biosafety requirements for the detection of Kunming virus, the collection, transportation, and storage of specimens, the processing of specimens, and fluorescence RT- PCR detection. This standard applies to the fluorescence RT-PCR detection of Kunjin virus.

2 Normative references

The following documents are indispensable for the application of this document. For dated references, only dated versions apply to this article Pieces. For undated references, the latest version (including all amendments) applies to this document. GB 19489 General Requirements for Laboratory Biosafety List of Pathogens of Human Infection (Ministry of Health, Health Science, Education and Education [2006] No. 15)

3 Terms and Definitions

The following terms and definitions apply to this document. 3.1 Kunjin virus Kunjinvirus; KUNV It can also be translated as “Kutchin Virus”, a member of the Flaviviridae Flavivirus genus, and in Culexan in Australia in 1960. First isolated in nulirostris). In addition to Culex pipiens pallens, Culex seudovishnui, Culex quinquefasciatus (Culex) Quinquefasciatus), Culex australis and Culex suamosus can also spread the disease poison. The main hosts of the virus are birds, poultry, pigs, cattle, and horses. Waterfowls can spread the virus to distant places, while pigs, cows and horses can enlarge The role of the host. Kunjin virus disease is a zoonotic disease, mainly in Australia. After human infection, it mainly manifests as fever, headache, Muscle pain, severe cases can develop encephalitis.

4 Abbreviations

The following abbreviations apply to this document. Ct value The number of cycles experienced when the fluorescence signal within each reaction tube reaches the set threshold; FAM FAM fluorescent dye, a fluorescent reporter group; RNA RNA RT-PCR reverse transcription-polymerase chain reaction.

5 Biosafety requirements

The List of Pathogens Infected by Humans stipulates that the operation of uninfected infectious substances and inactivated materials of Kunjin virus should be different. Level biosafety laboratories, as follows. ......

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